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Offlinedrteknikal
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Registered: 01/08/17
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Last seen: 7 years, 18 days
colonized rye berry jars don't produce sclerotia
    #23993179 - 01/08/17 05:54 PM (7 years, 22 days ago)

have several jars of prepared rye berries (out-grow.com source) fully colonized with several strains. no signs of any contamination. was trying to produce truffles, but after 3, 6, or 12 months, nothing.

first question is what went wrong? kept in cabinet at 68-72f. some looked like they were just short of pinning, most are just white fuzzy/thready mass.

second question is how to make something from nothing. I don't have a glove box handy, nor a temp/humidity controlled fruiting chamber. I assume the next step would be casing and fruiting, but not sure how to do a sterile transfer or fruiting with what I have.


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InvisiblemushboyMDiscord
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Re: colonized rye berry jars don't produce sclerotia [Re: drteknikal]
    #23993222 - 01/08/17 06:12 PM (7 years, 22 days ago)

Quote:

drteknikal said:
have several jars of prepared rye berries (out-grow.com source) fully colonized with several strains.




which strains?


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Offlinedrteknikal
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Re: colonized rye berry jars don't produce sclerotia [Re: mushboy]
    #23993478 - 01/08/17 07:28 PM (7 years, 22 days ago)

tampa, atl7, jalisco, and mex a


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InvisiblebodhisattaMDiscordReddit
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Re: colonized rye berry jars don't produce sclerotia [Re: drteknikal]
    #23993546 - 01/08/17 07:47 PM (7 years, 22 days ago)

Once jars are colonized you don't have to be sterile or clean anymore.
You don't need a temp controlled fruiting chamber.
And humidity is automatically handled by most passive fruiting chambers
You want a still air box not a glove box.


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Offlinedrteknikal
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Re: colonized rye berry jars don't produce sclerotia [Re: bodhisatta]
    #23993588 - 01/08/17 07:59 PM (7 years, 22 days ago)

nice. I was thinking about mixing with a substrate in bags. what's proper ratio for colonized grain to substrate and what's best substrate to use. I have a box I can use if I have to (gloves, but no seal, but can clean and let stand)...


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OfflineKillerclowns87
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Re: colonized rye berry jars don't produce sclerotia [Re: drteknikal]
    #23993783 - 01/08/17 08:57 PM (7 years, 22 days ago)

Quote:

drteknikal said:
what's best substrate to use.





In my research CVG (coir verm gysum) is one of the best for beginners in that its more cantam resistant than other substrates
and its easily obtainable Goodluck!:thumbup:


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