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gigi1618
Stranger
Registered: 04/12/15
Posts: 16
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Brf spawning wbs?
#23992080 - 01/08/17 10:43 AM (7 years, 22 days ago) |
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If I prep some wbs and use a crumble from a colonized brf cake, will it spawn to the wbs? Then I could monotub that, right? Should I still poke a hole and fill it with poly fill in the wbs jar if this is a reasonable idea? I'm afraid of contamination from the Verm layer on the brf. If I do the transfer in an SAB then can i knock off the Verm in the SAB and call it sterile procedure?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Brf spawning wbs? [Re: gigi1618]
#23992116 - 01/08/17 10:55 AM (7 years, 22 days ago) |
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Look up pf slurry. Better then just crumbled cake bits inside grains. Remember! Grains are more prone to bacterial issues then cakes.
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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: Brf spawning wbs? [Re: mushboy]
#23992137 - 01/08/17 11:08 AM (7 years, 22 days ago) |
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Quote:
Lobi said: here is half a pf cake to oats after 3 days. I have split it up to one cake to 4 qt jars of grain and it work out decently. I dump the dry verm layer in sab into an empty jar and dump the cake into a big ziploc bag and crush it up. then i pour it from the bag into the grain jar. you can also use Lazy Mofo Bag Tek and then crush that up and split it into 10 jars of grain and that should colonize in 4 or 5 days as well. i have had great success with this just to see if it works. I would recommend just doing regular g2g with agar masters tho..

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gigi1618
Stranger
Registered: 04/12/15
Posts: 16
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Re: Brf spawning wbs? [Re: Lobi]
#23992143 - 01/08/17 11:11 AM (7 years, 22 days ago) |
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Awesome thanks
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Salamander Dan
Stranger

Registered: 01/08/17
Posts: 1
Last seen: 6 years, 10 months
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Re: Brf spawning wbs? [Re: gigi1618]
#23992157 - 01/08/17 11:17 AM (7 years, 22 days ago) |
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How do I make my own post?
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Quote:
Salamander Dan said: How do I make my own post?
You just did? Unless you mean start a thread, you do that by going on the main page of cult near the top right of the screen theres a icon that says pot click that and it will start a thread
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