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puppetmastah
Stranger
Registered: 01/07/17
Posts: 51
Last seen: 3 years, 8 months
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Failed Attempts at Inoculation
#23991113 - 01/07/17 10:02 PM (7 years, 23 days ago) |
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There are two times that I tried inoculating, once putting the jars on the oven and using the heat to try and sterilize everything, and second just in my room.
What is the cleanest place in the house to inoculate? Or what can I do to make sure that another batch is not infected? I'm afraid because I have dogs and they have pooped in the house over the past years and worried that I can never ever grow mushrooms because of this issue. I heard it wasn't a good idea to do it outside either.
How can I make sure that the syringe itself is clean as well? I would really like to experience these things.
If anyone could help me out, I would be greatly appreciative! Happy New Year!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Failed Attempts at Inoculation [Re: puppetmastah] 1
#23991116 - 01/07/17 10:03 PM (7 years, 23 days ago) |
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 both yur answers, a sab and agar
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Failed Attempts at Inoculation [Re: cronicr]
#23991143 - 01/07/17 10:11 PM (7 years, 23 days ago) |
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puppetmastah
Stranger
Registered: 01/07/17
Posts: 51
Last seen: 3 years, 8 months
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Re: Failed Attempts at Inoculation [Re: bodhisatta]
#23991200 - 01/07/17 10:29 PM (7 years, 23 days ago) |
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Thank you so much you two! But even with this technique, it should work right?
There is no way that I can contaminate and fail simply by bringing the jars into my house?
If I set it down on the table, what if the air that I sat them in carried anything harmful to the growth of the mycelium? or am I worrying too much?
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WeavieWonder
Time Travel Sucks



Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 1 month, 4 days
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Re: Failed Attempts at Inoculation [Re: puppetmastah]
#23991222 - 01/07/17 10:40 PM (7 years, 23 days ago) |
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You've got worry warts. There is a lot of info on sterile technique in this forum. Read up.
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puppetmastah
Stranger
Registered: 01/07/17
Posts: 51
Last seen: 3 years, 8 months
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Re: Failed Attempts at Inoculation [Re: WeavieWonder]
#23991240 - 01/07/17 10:50 PM (7 years, 23 days ago) |
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Thank you Weavie.
Would a Portable UV-C Light Sterilizer suffice for safe inoculation?
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Failed Attempts at Inoculation [Re: puppetmastah]
#23991274 - 01/07/17 11:11 PM (7 years, 23 days ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 01:09 PM)
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BigPhoot
Grand Pooba



Registered: 07/20/16
Posts: 101
Last seen: 3 years, 11 months
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Re: Failed Attempts at Inoculation [Re: puppetmastah]
#23991276 - 01/07/17 11:13 PM (7 years, 23 days ago) |
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The best room is the smallest room because it's the easiest to sterilize. Mix bleach 10:1 and spray that in the air after wiping all surfaces with rubbing alcohol. Same goes with sterilizing the needle, rub with alcohol and then flame sterilize. Other than that the still air box is definitely the way to go. They're cheap and easy to make and are totally worth it.
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 The Aliens Breathe Nitrogen - Rep That O8 Brotha
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fungusfun
Changeling



Registered: 10/15/16
Posts: 29
Loc: Virgo Supercluster
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Re: Failed Attempts at Inoculation [Re: amidogen]
#23991292 - 01/07/17 11:22 PM (7 years, 23 days ago) |
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Quote:
amidogen said: And your dog's shit doesn't matter. Everyone's air, with or without dogs, is filthy. The SAB helps you keep contams from landing on your media 
Yeah, there is fecal coliform bacteria literally everywhere in your house. Check the mythbusters:
SAB solved all my problems. My first two attempts went wrong, big time. Then I got RR's DVD's and followed directions. 24/24 last 2 tries.
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puppetmastah
Stranger
Registered: 01/07/17
Posts: 51
Last seen: 3 years, 8 months
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Re: Failed Attempts at Inoculation [Re: BigPhoot]
#23991295 - 01/07/17 11:23 PM (7 years, 23 days ago) |
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Thank you very much you guys!! I appreciate it!
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Failed Attempts at Inoculation [Re: BigPhoot]
#23991300 - 01/07/17 11:26 PM (7 years, 23 days ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 01:09 PM)
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WeavieWonder
Time Travel Sucks



Registered: 08/18/16
Posts: 1,455
Loc: USA
Last seen: 1 month, 4 days
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Re: Failed Attempts at Inoculation [Re: BigPhoot]
#23991304 - 01/07/17 11:27 PM (7 years, 23 days ago) |
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Quote:
BigPhoot said: Mix bleach 10:1 and spray that in the air
Just no.
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puppetmastah
Stranger
Registered: 01/07/17
Posts: 51
Last seen: 3 years, 8 months
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Re: Failed Attempts at Inoculation [Re: WeavieWonder]
#23993867 - 01/08/17 09:31 PM (7 years, 22 days ago) |
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If the smallest place in the house is the bathroom should I still be against inoculating in there?
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Psilosoulful
Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Failed Attempts at Inoculation [Re: puppetmastah]
#23993882 - 01/08/17 09:36 PM (7 years, 22 days ago) |
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You're much better off just making a SAB and following sterile procedures
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Failed Attempts at Inoculation [Re: Psilosoulful]
#23994011 - 01/08/17 10:43 PM (7 years, 22 days ago) |
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x9000
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