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AldoTheApache
Stranger
Registered: 04/29/20
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Thanks for the tek! Building mine tomorrow!
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Phytrix
Stranger
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
#26653943 - 05/06/20 09:47 PM (3 years, 8 months ago) |
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Are you still of the impression that, when inoculating jars, it's best to remove the syringe and flame sterilise after each jar? Apologies in advance if I have your impression mixed up with someone elses. Every time you do this, some air would have to come in to replace where your arms were, inviting in unsettled bacteria and creating some form of light draft. Say I had a dozen jars in a SAB, what would you say to doing lots of 4 at a time before re-sterilising your needle?
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Ababyphoenix
You



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Posts: 1,015
Loc: Human Body
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
#26663884 - 05/11/20 06:28 AM (3 years, 8 months ago) |
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Quote:
bodhisatta said:
I believe my work speaks for the efficacy of not needing to over complicate things.
now get to work!
--------------------
   Pho's MushiAventures:
My '21 GLOG LAGM 2022
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shroom2020
Stranger
Registered: 05/10/20
Posts: 39
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Ababyphoenix]
#26666445 - 05/12/20 12:23 PM (3 years, 8 months ago) |
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deleted
Edited by shroom2020 (05/24/20 01:00 PM)
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Poolofsouls
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: shroom2020]
#26668096 - 05/13/20 06:59 AM (3 years, 8 months ago) |
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So it's ok to torch your spore syringe outside the sab before inoculating?
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poisoned
untitled



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Loc: Yurop
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Poolofsouls] 1
#26668109 - 05/13/20 07:11 AM (3 years, 8 months ago) |
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Quote:
Poolofsouls said: So it's ok to torch your spore syringe outside the sab before inoculating?
That's the correct way. You shouldn't have any fires inside a SAB.
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shroom2020
Stranger
Registered: 05/10/20
Posts: 39
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: poisoned]
#26668485 - 05/13/20 11:32 AM (3 years, 8 months ago) |
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deleted
Edited by shroom2020 (05/24/20 01:00 PM)
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poisoned
untitled



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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: shroom2020]
#26668524 - 05/13/20 12:03 PM (3 years, 8 months ago) |
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Well, a burner running inside your SAB makes it not-so-still-AB.
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Domno
Magician



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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: poisoned]
#26668619 - 05/13/20 01:16 PM (3 years, 8 months ago) |
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Quote:
poisoned said: Well, a burner running inside your SAB makes it not-so-still-AB.
Hadn't thought about the turbulence it would create. I just do it outside because I spray all my stuff down with alcohol before putting in the box and i dont want to blow myself up with the fumes.
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Ababyphoenix
You



Registered: 03/21/19
Posts: 1,015
Loc: Human Body
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Domno]
#26668792 - 05/13/20 03:26 PM (3 years, 8 months ago) |
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Quote:
Domno said:
Quote:
poisoned said: Well, a burner running inside your SAB makes it not-so-still-AB.
Hadn't thought about the turbulence it would create. I just do it outside because I spray all my stuff down with alcohol before putting in the box and i dont want to blow myself up with the fumes.
Ur wasting Iso and potential health. Just use very diluted soapy water like the tech calls for. There's absolutely no reason to use Iso on a SAB other than bad practice and potential misunderstanding.
Quote:
bodhisatta said: You CANNOT sterilize the inside of a SAB Sanitizers are not Sterilizers and Sanitary is not sufficiently contamination free to do STERILE work. thus you have to work with physics rather than your chemicals
--------------------
   Pho's MushiAventures:
My '21 GLOG LAGM 2022
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VernsMB
RRV Redneck

Registered: 05/13/20
Posts: 10
Loc: Manitoba CA
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Ababyphoenix]
#26669305 - 05/13/20 07:49 PM (3 years, 8 months ago) |
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What is the minimum dimensions of the Tub for a SAB so you do your pour without to much restriction?
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Ababyphoenix
You



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Posts: 1,015
Loc: Human Body
Last seen: 24 days, 3 hours
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: VernsMB] 1
#26669326 - 05/13/20 07:55 PM (3 years, 8 months ago) |
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Quote:
VernsMB said: What is the minimum dimensions of the Tub for a SAB so you do your pour without to much restriction?
Learning to use the Search Function will bring u so far friend. I refer you to the general rules post rule #1
I bought the biggest fucking SAB i could i think like 70+ qts. I jsut bought the biggest clear tote in the store and i dont regret it.
--------------------
   Pho's MushiAventures:
My '21 GLOG LAGM 2022
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agentm00se
Stranger
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
#26680448 - 05/19/20 06:17 AM (3 years, 8 months ago) |
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awesome, thank you man <3
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Crazydiamond311
Stranger
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
#26775444 - 06/22/20 09:05 PM (3 years, 7 months ago) |
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I followed this Tek recently and have had great success. Very little contamination when working with agar or grain. Some small instances of contamination on my plates but I am new and still getting my movements down to reduce air disturbance. It can be intimidating to pour plates the first few times but I am already feeling more comfortable and getting better. Decided to jump straight into the deep end with Agar and Bod's unmodified tub Tek and was surprised at my success. Thanks for all the great info.
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spandoos
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Registered: 05/26/20
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Thanks for all the great info
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polko
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
#26818423 - 07/11/20 11:00 PM (3 years, 6 months ago) |
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Great guide for a nwebie. Thanks from Argentina!
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retowen
Forest Dweller
Registered: 08/05/16
Posts: 51
Loc: Canada
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: polko]
#26818439 - 07/11/20 11:22 PM (3 years, 6 months ago) |
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A wonderful Tek indeed! Thank you!
-------------------- Trade List
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MrTinAZ
If only I knew



Registered: 07/01/20
Posts: 138
Loc: slightly north of Mexico
Last seen: 3 years, 5 months
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
#26883376 - 08/16/20 12:13 PM (3 years, 5 months ago) |
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Thank you so much for this TEK! There are tons of great threads I want to thank people for, but they are generally many years old and I don't want to necro bump and old thread. I know this thread is old, but since people posted on it just last month I figure it is not bad ettiquette to thank you for the TEK!
I could not find a 5" coffee can, I bought a small coffee can but it was only 4" so I had to go back and get a bigger one. The small 4" can was an 11.3" can and I would recommend avoiding these unless you have small hands/arms. I am a pretty big guy so I got the 6" can of Hills Brothers (because it was on sale for 5.99 instead of $9+ like others). The 6" can is 23 oz (1 lb 7 oz).
I'd recommend getting one bigger than the 4" so either look at the oz size based on what I am saying or just bring your tape measurer to the store to be sure you don't make my mistake of buying 1 too small. If you can find a can with a 5" size that could work great but I could not so I went with the 6". Hopefully, it is not TOO big because that could let extra air in I don't want, but I think it will work fine since I am 6'3" 220lbs so I may need a bigger opening than some people.
TLDR make sure to check the size of the can you buy so that you don't make the mistake I did and buy a can that is too small! And THANK YOU bodhisatta for this method! 
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vencx
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
#26952000 - 09/23/20 10:54 PM (3 years, 4 months ago) |
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Thank you Bod!! I love everything that is Simple as fuck!
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lord_nikon6983
Seeker


Registered: 08/10/20
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
#26953115 - 09/24/20 05:11 PM (3 years, 4 months ago) |
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Thank you very much! I really like the use of the can to create the arm holes. I was balking at the price of the proper sized hole saw bit.
-------------------- Not all those who wander are lost
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