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InvisibleAlchemycologist
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
    #26150731 - 08/24/19 11:00 AM (4 years, 5 months ago)

Right, but the SFDs I'm talking about will rip and flake off material if you try to wipe them while wet. They're fine if you don't fuck with them but I've had them get wrecked while wiping them with alcohol.

Again, my SFDs are crap and I wouldn't buy them again but someone else might have the same problem.


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Offlinejbgtaa
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Alchemycologist]
    #26151584 - 08/24/19 10:47 PM (4 years, 5 months ago)

I’m sorry to add to this off-topic conversation, but I’ve heard that you should try to keep polyfil lids as dry as possible because if they’re wet they can grow contaminated. Is there any truth to this? After the pc cycle I mean, obviously they’re saturated with steam and sterilized in the pc


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: jbgtaa]
    #26151653 - 08/24/19 11:50 PM (4 years, 5 months ago)

They'll dry out when you remove them from the cooker


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Offlinetryptkaloids
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
    #26151660 - 08/25/19 12:04 AM (4 years, 5 months ago)

I've fucked up sfds by leaving them in the cooker for a month. Just don't do that and you'll be good


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Offlinejbgtaa
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: tryptkaloids]
    #26151735 - 08/25/19 01:50 AM (4 years, 5 months ago)

Usually i take my jars out about 3 minutes after the top drops on the pc w a jar handler, tighten the lid, crunch the foil a bit around the band, and return the the cardboard jar box. shake thoroughly right before a2g/g2g.  i assumed they steamed off, im just having some problems with like 1/6 jars going green and im trying to convince myself it's anything but my grow area... (wet basement, huge sporeload i'd imagine).


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Offlinetryptkaloids
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: jbgtaa]
    #26151750 - 08/25/19 02:11 AM (4 years, 5 months ago)

Don't leave the foil on and make your poly linds tighter, that's the problem with poly, its inconsistent. Also check all your other factors like technique, grain prep and anything on your jar rings or threads that could wick contams into


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
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Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineGwindor
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
    #26151769 - 08/25/19 02:30 AM (4 years, 5 months ago)

Thank you!
And what about petri dishes. If I want to inoculate agar in petri dishes using inoculation loop. Is it ok, if petri dishes will be wet and soapy? I mean soapy water can be leaked inside petri dishes...


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InvisibleBlazer420
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Gwindor]
    #26151794 - 08/25/19 03:09 AM (4 years, 5 months ago)

Is it true a dry SAB can be more contam free than a wet soapy SAB?

Trying to get the best out of my SAB, even went out of the way to get a metal screen for the bottom of my SAB to keep objects inside the SAB off of the ground.

Should I just spray the bottom with some soapy water? Or is a dry SAB best?
There can be some rather conflicting information on here haha.


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Edited by Blazer420 (08/25/19 03:17 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Gwindor]
    #26151984 - 08/25/19 07:45 AM (4 years, 5 months ago)

Quote:

Gwindor said:
Thank you!
And what about petri dishes. If I want to inoculate agar in petri dishes using inoculation loop. Is it ok, if petri dishes will be wet and soapy? I mean soapy water can be leaked inside petri dishes...



Put the dishes in after you spray then lol.
Besides were talking a quick light mist not a drenching


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OfflineGwindor
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta] * 1
    #26161878 - 08/31/19 05:17 AM (4 years, 4 months ago)

As I understand the point of SAB is that inside surfaces of SAB become wet and soapy, and contaminants from air stick to all wet and soapy surfaces.

If I preload all working stuff in SAB before spraying soapy water inside, that means all working stuff will have soapy surfaces sticking contaminants.

For example, if I put jars and spore syringe inside the SAB and then spray inside. My syringe and jars surfaces will become soapy and will get contaminants from the air sticked to them.
And will I need to touch jars and syringe carefully and wipe my hands with alcohol after touching them each time, because their surfaces are "dirty" and full of contaminants?

I mean, I'm trying to imagine process, and it seems to me, that jars and syringe will become kind of "dirty" if I spray soap water when they're inside the SAB.

Do I understand the mechanics correctly?


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Gwindor] * 1
    #26162036 - 08/31/19 09:15 AM (4 years, 4 months ago)

I don't even bother spraying my SAB at all anymore.


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InvisibleBlazer420
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
    #26165496 - 09/02/19 03:07 PM (4 years, 4 months ago)

Yeah I hear dry is the way to go these days, and also having a metal wire rack at the bottom 2 help lift things off from the "ground"


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OfflineKindOne
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Blazer420]
    #26170675 - 09/05/19 01:24 PM (4 years, 4 months ago)

Thank you so much! That coffee can hole cutter is the only way to go.


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OfflineEdmunter
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: bodhisatta]
    #26171651 - 09/05/19 11:43 PM (4 years, 4 months ago)

Quote:

bodhisatta said:
I don't even bother spraying my SAB at all anymore.




Really?  What is your procedure then if u dont mind explaining?


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OfflineEdmunter
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Gwindor]
    #26171656 - 09/05/19 11:48 PM (4 years, 4 months ago)

Quote:

Gwindor said:
As I understand the point of SAB is that inside surfaces of SAB become wet and soapy, and contaminants from air stick to all wet and soapy surfaces.

If I preload all working stuff in SAB before spraying soapy water inside, that means all working stuff will have soapy surfaces sticking contaminants.

For example, if I put jars and spore syringe inside the SAB and then spray inside. My syringe and jars surfaces will become soapy and will get contaminants from the air sticked to them.
And will I need to touch jars and syringe carefully and wipe my hands with alcohol after touching them each time, because their surfaces are "dirty" and full of contaminants?

I mean, I'm trying to imagine process, and it seems to me, that jars and syringe will become kind of "dirty" if I spray soap water when they're inside the SAB.

Do I understand the mechanics correctly?




here are a couple of ways that I have done it.  First is to put stuff in the sab, cover with iso soaked paper towels and spray then work

Or spray, add jars/dishes str8 from the pc then work

Regarding syringes a liberal wipes down is ok but im mainly concerned with the needle that should be flame sterilised anyway


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OfflineKindOne
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Edmunter]
    #26171948 - 09/06/19 06:56 AM (4 years, 4 months ago)

Okay, what’s SFD? (Yes, I looked for it in the glossary.) Thanks!


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OfflineCthulhu23
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: KindOne]
    #26171964 - 09/06/19 07:12 AM (4 years, 4 months ago)

Synthetic filter disk.  It’s used as a way to ensure gas exchange without allowing contaminants into your jars.  Used instead of polyfill.


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OfflineKindOne
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Cthulhu23]
    #26172002 - 09/06/19 07:42 AM (4 years, 4 months ago)

Thank you.


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Offlinejbgtaa
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: Edmunter]
    #26173526 - 09/07/19 02:08 AM (4 years, 4 months ago)

Quote:

Edmunter said:
Quote:

Gwindor said:
As I understand the point of SAB is that inside surfaces of SAB become wet and soapy, and contaminants from air stick to all wet and soapy surfaces.

If I preload all working stuff in SAB before spraying soapy water inside, that means all working stuff will have soapy surfaces sticking contaminants.

For example, if I put jars and spore syringe inside the SAB and then spray inside. My syringe and jars surfaces will become soapy and will get contaminants from the air sticked to them.
And will I need to touch jars and syringe carefully and wipe my hands with alcohol after touching them each time, because their surfaces are "dirty" and full of contaminants?

I mean, I'm trying to imagine process, and it seems to me, that jars and syringe will become kind of "dirty" if I spray soap water when they're inside the SAB.

Do I understand the mechanics correctly?




here are a couple of ways that I have done it.  First is to put stuff in the sab, cover with iso soaked paper towels and spray then work

Or spray, add jars/dishes str8 from the pc then work

Regarding syringes a liberal wipes down is ok but im mainly concerned with the needle that should be flame sterilised anyway




In theory, as long as everything in your sab is *moderately* clean, and you dont move too swiftly and knock shit around, all that is overkill. Bod probably doesnt spray his sab because, i would assume, he works in a moderately clean area. I, however, work in an area with a very high mold sporeload, therefore I spray my sab to ensure that whatever enters it gets stuck to the walls. And NOTHING clean ever touches the walls. I use disposable scalpel tips and sterilized syringes, so i dont use any flame whatsoever.


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OfflineEdmunter
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Re: BOD's SIMPLE AS FUCK SAB TEK [Re: jbgtaa]
    #26173533 - 09/07/19 02:18 AM (4 years, 4 months ago)

Quote:

jbgtaa said:
Quote:

Edmunter said:
Quote:

Gwindor said:
As I understand the point of SAB is that inside surfaces of SAB become wet and soapy, and contaminants from air stick to all wet and soapy surfaces.

If I preload all working stuff in SAB before spraying soapy water inside, that means all working stuff will have soapy surfaces sticking contaminants.

For example, if I put jars and spore syringe inside the SAB and then spray inside. My syringe and jars surfaces will become soapy and will get contaminants from the air sticked to them.
And will I need to touch jars and syringe carefully and wipe my hands with alcohol after touching them each time, because their surfaces are "dirty" and full of contaminants?

I mean, I'm trying to imagine process, and it seems to me, that jars and syringe will become kind of "dirty" if I spray soap water when they're inside the SAB.

Do I understand the mechanics correctly?




here are a couple of ways that I have done it.  First is to put stuff in the sab, cover with iso soaked paper towels and spray then work

Or spray, add jars/dishes str8 from the pc then work

Regarding syringes a liberal wipes down is ok but im mainly concerned with the needle that should be flame sterilised anyway




In theory, as long as everything in your sab is *moderately* clean, and you dont move too swiftly and knock shit around, all that is overkill. Bod probably doesnt spray his sab because, i would assume, he works in a moderately clean area. I, however, work in an area with a very high mold sporeload, therefore I spray my sab to ensure that whatever enters it gets stuck to the walls. And NOTHING clean ever touches the walls. I use disposable scalpel tips and sterilized syringes, so i dont use any flame whatsoever.




Lol, overkill.  Spray SAB add jars and work. 

Joking aside, I feel the first answer was for the fella who didnt now how to spray without worrying about it sticking to the lids and adding another vector.  Like you my work station is in a moldy old room. 

One other thing id like to mention is the speed and steadiness you move your hands at.  I move slowly into the SAB and make as few jerk movements as possible


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