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Offlinewtfcrazymofo
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Re: I've hit a fucking wall... [Re: Josex]
    #23993294 - 01/08/17 06:32 PM (7 years, 22 days ago)

Bust the fucking wall down. Temp swings? Scalpel scorch? shwag-gy dry agar Media?  Low RH?

Premix MEA FTW.


Edited by wtfcrazymofo (01/08/17 06:43 PM)


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InvisibleJosex
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Re: I've hit a fucking wall... [Re: wtfcrazymofo]
    #23993380 - 01/08/17 06:54 PM (7 years, 22 days ago)

What do you mean by scalpel scorch?


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Offlinewtfcrazymofo
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Re: I've hit a fucking wall... [Re: Josex]
    #23993433 - 01/08/17 07:11 PM (7 years, 22 days ago)

A hot scalpel into fresh mycelium.  Probably not the problem.  Anti- fungal potato flakes maybe, but just guessing.


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Bag sealers are to bulky (my hood isn't that big)
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InvisibleComebackKid
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Re: I've hit a fucking wall... [Re: Josex]
    #23993471 - 01/08/17 07:24 PM (7 years, 22 days ago)

Damn dude that really fuckin blows. :sad:
I always use my mini electric PC for no pour but I got a 23 qt pressure canner recently and I was suprised to find that my grain jars were still hot in the morning.

You may be onto something with the overcooking... I hope you figure it out soon man


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InvisibleJosex
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Re: I've hit a fucking wall... [Re: ComebackKid]
    #23993631 - 01/08/17 08:09 PM (7 years, 22 days ago)

Thanks cbk, really appreciate the encouraging words. It's been a nightmare but I'm gonna bust the wall down if it's the last thing I do :banghead:
Bright side is that now I've got a lot of practice under my belt, nuttin wrong with that.

I'm pretty sure is something to do with  leaving the plates in the pc, it maintains the heat inside for a very long time, something tells me that can't be good.

Yeah mofo, I do tend to overdo it when I heat the scalpel but I cool it well on the receiving plate, even then I hear a sizzling sound when I cut the wedge but there's a lot of myc still intact on the wedge when I transfer it.


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Offlinelovelaughlibs
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Re: I've hit a fucking wall... [Re: Josex]
    #23994048 - 01/08/17 11:06 PM (7 years, 21 days ago)

I can relate. Been trying to get my agar to work since October by now. Every batch just grew nothing at all, not even contams. Current batch has finally shown some tiny white dots after 10 days of waiting but if they turn out to be contams after all this wait I'm fuckin done.


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OfflineUnknown error
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Re: I've hit a fucking wall... [Re: Josex]
    #23995288 - 01/09/17 01:40 PM (7 years, 21 days ago)

Quote:

Josex said:
I know the feel man, it's puzzling and frustrating as hell. I hope you find a way to make it work. Your wedges are even lazier than mine and that's saying something.

If you have the time I'd love to read all the steps you do to whip up a batch of agar and maybe we can find something that looks sketchy.

I don't think it's your agar though, looks like the real deal.
This is the one I use:


In the ingredients you can read E406, but I looked it up and that is just the additive number for agar agar, so there can't be anything wrong with my agar either.




Yeah really frustrating when it's seems your doing things the right way and it's still not working.
The mea recipe I used was 9g agar, 10g lme and and 500ml of water.
The pda was 9 g agar, 5g potato flakes, 10 ml honey and 500ml water.
Both pc'ed for 30 mins @ 15 psi. I done pour and no pour, the pour was taken out soon after the pressure dropped to cool. The no pour left in overnight.
I don't think there's anything there that should really stop it from being ok, but there must be something up with it.
Iv made two more batches over the weekend, some mea with half the amount of agar. That still seemed to set ok and some premixed pda that I had left over, just add water. I know that works Iv Used it before.
So il give them a try. Hope you have better luck with your new batch


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OfflineTiamo
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Re: I've hit a fucking wall... [Re: Josex]
    #24006129 - 01/13/17 02:47 AM (7 years, 17 days ago)

I've been having the exact same issue and it's driving me absolutely crazy.

Cakes take 7-8 weeks, petri dish takes 3-4 weeks to get to the size of a 10 cent coin, LCs don't seem to grow at all, rye jars take 7 days to reach 1% colonisation and seem to stall.

The weird thing is all my growth seems to happen in spurs. Everything will grow really fast for a few days, then completely stall for a week. I don't know what to do anymore. :mad2:

Edit: Could it be my water? That's the only common thing in all of these.


--------------------


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Edited by Tiamo (01/13/17 03:35 AM)


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InvisibleJosex
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Re: I've hit a fucking wall... [Re: Tiamo]
    #24006207 - 01/13/17 04:31 AM (7 years, 17 days ago)

You're using distilled water right? You have to be careful there, I did a bit of research about it and apparently there are some types of distilled water that contains shit that's not good for you and if you can't drink it, neither can the mycelium.

Type the brand of your distilled water on google and if you see that people use it for their fish tanks, then it's good to go, otherwise buy another brand.

As for me, my problem was almost certainly the pc. I'm not 100â„… sure yet but I've made new batches that were taken out of the pc when the pressure reached zero and the consistency of my agar feels entirely different now and I'm already seeing growth fron spores at a much faster speed. Touching wood here though.


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OfflineTiamo
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Re: I've hit a fucking wall... [Re: Josex]
    #24006227 - 01/13/17 04:53 AM (7 years, 17 days ago)

No, I used to but I ran out. Now I am using tap. It should be absolutely fine, especially for cakes and rye. :justdontknow:

This is a weeks worth of growth on rye. I inoculated with an agar wedge the size of 1 euro coin, shook it and the mycelium and agar disappeared. Then growth happened. Now nothing is happening.



--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisibleJosex
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Re: I've hit a fucking wall... [Re: Tiamo]
    #24006445 - 01/13/17 07:40 AM (7 years, 17 days ago)

Wow that sucks man, hate to see you go through this. It's weird that it happens with everything you seem to be doing, not only agar.
But cheer up for fuck's sake man, you're being very hard on yourself with that title.

You have a lot of potential, it's true, not trying to flatter you here and I'm sure it must be some silly thing that's nothing to do with your skills and you're gonna figure it out soon :super:


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OfflineTiamo
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Re: I've hit a fucking wall... [Re: Josex]
    #24006459 - 01/13/17 07:48 AM (7 years, 17 days ago)

Yea, I hope so. I've been feeling a little down about it, honestly. All I want to be happy is a jar full of clean rye spawn and a few colonised agar plates. I'll change that title. :thumbup:


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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OfflineCrispykoot
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Re: I've hit a fucking wall... [Re: Josex]
    #24006499 - 01/13/17 08:11 AM (7 years, 17 days ago)

Quote:

Josex said:
Thanks cbk, really appreciate the encouraging words. It's been a nightmare but I'm gonna bust the wall down if it's the last thing I do :banghead:
Bright side is that now I've got a lot of practice under my belt, nuttin wrong with that.

I'm pretty sure is something to do with  leaving the plates in the pc, it maintains the heat inside for a very long time, something tells me that can't be good.

Yeah mofo, I do tend to overdo it when I heat the scalpel but I cool it well on the receiving plate, even then I hear a sizzling sound when I cut the wedge but there's a lot of myc still intact on the wedge when I transfer it.




Hey Josex for what its worth, I just experienced this with agar for the first time. The only real difference in batches is my PC seal fucked up due to ice, and I never got to pressure on this agar PC cycle. I noticed it, dropped the pressure, fixed the seal and re PC'd.

The agar was in a LOT longer and shit from that batch is not growing well. It's MEA and its clear so no caramelizing, but slow growth.Purely anecdotal, but might be onto something.


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InvisibleJosex
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Re: I've hit a fucking wall... [Re: Crispykoot]
    #24006597 - 01/13/17 09:06 AM (7 years, 17 days ago)

Will be watching ya grow those AA+ :cheers:


Edited by Josex (10/04/17 08:58 AM)


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InvisibleJosex
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Re: I've hit a fucking wall... [Re: Josex] * 1
    #24682945 - 10/04/17 09:37 AM (6 years, 3 months ago)

Some people have asked me I had finally figured out my issues with agar, the answer is yes, long ago.

It was just the agar powder I was using that for whatever reason only grew very tomentose myc that grew rather slowly, but the cultures themselves were perfectly ok to use and I did lots of grows using that agar.

It was ridiculous how thoroughly convinced I was about my agar powder being good, maybe it was because of how expensive it was, or the nice bottle, or whatever the hell. Thing is that in my mind the least plausible cause was the agar itsel, and only after having checked all the other possible variables that were causing this (some of them absurd) I finally became suspicious of the agar powder. A beautiful thing experience gives you is the ability to pinpoint problems more quickly and accurately, but that was the first real obstacle I encountered, so I saw ghosts everywhere.

Mrmazda recommended me an agar powder on Ebay and I bought it, and finally the cultures behaved as they were supposed to, growing rhizomorphically when they had to.

So, for anyone reading this that have similar issues; If for whatever reason all your different cultures present a common abnormality, then it is almost safe to say the problem doesn't lie with the cultures themselves. Change the way you're doing things, one variable at a time, in order to determine what the problem is.


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InvisibleMycolorado
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Re: I've hit a fucking wall... [Re: Josex]
    #24682999 - 10/04/17 10:10 AM (6 years, 3 months ago)

Did you happen to be using telephone co?  Ever since I started using it my cultures have been very tomentose...anecdotally, I'm convinced there is enough nutrients in it to steer cultures toward tomentose growth.  Also, it's hard as a rock at 2%...I'm using it at 1.25% and can't wait to be done with it...


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InvisibleJosex
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Re: I've hit a fucking wall... [Re: Josex]
    #24683092 - 10/04/17 11:19 AM (6 years, 3 months ago)

No, I wasn't using that agar. I'd tell you the one I currently use that works excellent but it's from a vendor based in the UK. Aren't you planning on replacing your agar-agar? I would not wait to be done with the one you have right now, shit's frustrating. Mine solidified very hard too.

This is the one that was giving me problems, enlarge the deleted pics to see them:
Quote:

Josex said:


In the ingredients you can read E406, but I looked it up and that is just the additive number for agar agar, so there can't be anything wrong with my agar either.



:hahthatscute:


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InvisibleMycolorado
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Re: I've hit a fucking wall... [Re: Josex]
    #24683108 - 10/04/17 11:28 AM (6 years, 3 months ago)

Right on...yeah, actually, I was shopping online yesterday, but hadn't decided on anything.  I've only had a couple rhizo plates pop up recently as opposed to pretty much every plate before using this shit...

Thanks for the input, Josex!


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Offlineicetech
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Re: I've hit a fucking wall... [Re: Mycolorado]
    #24683179 - 10/04/17 12:00 PM (6 years, 3 months ago)

wow, i didn't know the agar itself would cause issues.. since it is basically the gelling agent (unless i am wrong), mine is some "NOW!" brand from a knock off whole foods.. will see if its ok.


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OfflineJohnnyJohnson
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Re: I've hit a fucking wall... [Re: icetech]
    #24683308 - 10/04/17 01:00 PM (6 years, 3 months ago)

OP i had a similar problem to you. it took  my ass like 6 tries to finally get it right. The first 2 times i had shit spores. I got it right using a 10 gram agar/ 15 gram ELME ratio for every 500 ml of distilled h2o. gentamycin sulphate helps if ur able to get some (use .05 G).


Edited by JohnnyJohnson (10/04/17 08:41 PM)


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