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PlantManBee
undifferentiated



Registered: 11/17/16
Posts: 360
Last seen: 1 year, 6 months
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Bacterial syringe: how common and how big of a deal?
#23990749 - 01/07/17 08:05 PM (7 years, 23 days ago) |
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Hey y'all,
I'm quite new at growing Fungi and i've only ordered two spore syringes so far. My first attempt with an old spore print and the pf TEK was a complete bust as far as getting any shrooms. I learned many things NOT to do .
The next go round (now) was with a syringe of Golden Teachers. I did two small batches, the first one 6 jars. Each ended up with an average of about 0.8cc . The next batch of 5 I only put a drop into each of the four injection sites, after reading that the fewer number of strains in each cake the better. The first six all reached full colonization with little obvious bacteria, two of the last five colonized as fast as the first batch, two seem to have a bit of bacterial action going on.
I also made an SAB, got some agar, poured some plates and started playing with that on Xmas day. . I've done a few microbiology labs and was quite good at isolating microbes on agar. So it surprised me, first, how different putting water from a syringe on bouncy agar. I literally plopped a drop on the agar and it BOUNCED and rolled before the surface tension broke and it pooled on the Petri wall. . I put three drops on two plates that night (including the bouncing ball), and put them in a drawer and waited. about three days later it was obvious that they were pretty ugly. Yellowish bacteria. I figured I'd screwed up, was too stoned and not meticulous enough when I inoculated the plates. So, i decided I'd use an inoculation loop, made one, used it by simply flaming it, touching it to a drop of spore solution, press the loop slightly into the surface of the agar, and seal the dish.
The results were incubated and the results were yellowish bacteria only on the inoculation site. Still, I figured it was my technique.
So I decided to try two more plates using the needle but injected into the agar. This lead to the first plate being labeled "splat!" as I'm not so great at getting just a drop out lol. So did two more.
Well, I'm really glad that I didn't throw "splat!" out, because everywhere the spore water touched had bacterial colonies starting.
How common is this? Is BRF some sort of antibacterial miracle?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Bacterial syringe: how common and how big of a deal? [Re: PlantManBee]
#23990756 - 01/07/17 08:07 PM (7 years, 23 days ago) |
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cakes are not a miracle but it's harder for bacteria to travel around so usually you get some mycelium growth and they do fine
if you dilute your spore syringe or take a single drop onto an inoculation loop then "Swab" a dish you get much better results.
I've not had many bacterial syringes from vendors before but I would say at least 10% of them gave some problems. I've never ran into one that I couldn't get healthy growth from. it's harder to battle mold than bacteria in my experience. a syringe contaminated with rogue mold spores is a bitch.
I just put some PEU GT Treasure Coast and pan cyan spores to agar. and only a few of the treasure coast plates were bacteria where I put the inoculation loop I made 9 plates of each variety and only a couple of the TC ones put up a fight but there were plates that germinated and either over ran bacteria or didn't have any so it was no big deal
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PlantManBee
undifferentiated



Registered: 11/17/16
Posts: 360
Last seen: 1 year, 6 months
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Re: Bacterial syringe: how common and how big of a deal? [Re: bodhisatta]
#23991872 - 01/08/17 09:11 AM (7 years, 22 days ago) |
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Cool. Thanks for the reply. The next in line is a P. galindoi spore print
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