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OfflineBlueFire_ita
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Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo]
    #23987481 - 01/06/17 06:59 PM (7 years, 24 days ago)

Hello everyone. I'm here asking for quite specific advice for a cultivation, I tried to be as clear as possible in the title, if not I'll edit it.
I'll begin with premises, out you don't usually like them and want to go straight to the point read only after the photographs :smile:

For those who like the history behind things: I'm an Italian guy who, this year, is finishing high school studies (specialized in chemistry). We usually take, for the final exam, a theoretical or practical work, which, in my case, will be  psychoactive substances-based. You can't imagine the frustration of creating everything legally-doable (you can't, sadly work with psilocybin analytical standards, so in analytical chemistry I'll choose the maybe only legal option of datura's alkaloids), but, lucky me, of you check the controlled substances tables, you'll see that, even though they contains psilocybin, Panaeolus are perfectly growable.

SEMI-USEFUL PART: I'll try, then, to grow Panaeolus Goliat (the only strain whose spores are available for shipping from EU) and clone a single mushroom's mycelium. I would do everything at school, where there is a "thermal chamber" (used as incubator for Petri dishes) that I can use, since the part of program involving bacteria is usually finished until February and my exam is in June. I have made some picture of it (if you need higher higher resolution just ask):


(That's to give an idea about dimensions. I'm ~1.70-80m)




So, my problem is: my teacher said that the temperature can go from room to ~50°C, and that's fine, but how could I regulate humidity and light? Is it possible? Is there anything I should know about a professional incubator?
Do you think there's anything to say I didn't mentioned?

Thank you very much,
Peace, love and psilocybin :heart:


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OfflinePinPornProducer
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: BlueFire_ita] * 1
    #23987504 - 01/06/17 07:07 PM (7 years, 24 days ago)

Imo getting that thing to growing conditions would be way more work than its worth.


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Invisibleberlinmyc
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: PinPornProducer]
    #23988193 - 01/06/17 11:37 PM (7 years, 23 days ago)

Are you going to fruit in the incubator or just colonize agar and spawn?


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Invisibleblindingleaf
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: berlinmyc]
    #23988576 - 01/07/17 05:32 AM (7 years, 23 days ago)

hey OP, all that stuff is really cool, but beware of maybe accidentally growing mold in that incubator and it infecting other projects unrelated (if any are in there)

the hood will be nice to have for transfers, but the incubator might do more harm then good.

don't forget that besides light, humidity, and temperature, co2 will play a role in performance.  those incubators look like there will be enough light penetration without adding more inside.

measuring the exact PPM isn't necessary to succeed, but would be interesting if u could.  the important part is recognizing that air should be moved around and replaced either by active or passive means during fruiting.


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MICROBIAL HUSBANDRY!!!!

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OfflineBlueFire_ita
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: berlinmyc]
    #23988582 - 01/07/17 05:38 AM (7 years, 23 days ago)

Quote:

PinPornProducer said:
Imo getting that thing to growing conditions would be way more work than its worth.



I agree, but since it's a school work and, mostly, since I have to pay everything, I thought that it should look professional and be as cheap as possible...
Anyway: I can easily isolate the 2 half puff the incubator.

Quote:

berlinmyc said:
Are you going to fruit in the incubator or just colonize agar and spawn?



I have read that having mushrooms directly from agar is quite advanced, and since its my first attempt to grow something (thank you law, we all love you...) my idea was fruiting un the incubator and then use agar to make some clones of a random lucky shroom. (Which I'll make (or not) fruit depending on how much time I'll have before the exams.


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Invisibleblindingleaf
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: BlueFire_ita]
    #23988589 - 01/07/17 05:44 AM (7 years, 23 days ago)

I've never seen pans pin on agar personally, and I have tried.

I know Muda has a pic of it happening, and maybe stonesun.  so its not that it won't happen, its just that u might be waiting a long time if ur going that route.

if thats the case, u won't need to worry about co2 or humidity.  just light and temp


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineBlueFire_ita
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: blindingleaf]
    #23988597 - 01/07/17 05:50 AM (7 years, 23 days ago)

Quote:

blindingleaf said:
hey OP, all that stuff is really cool, but beware of maybe accidentally growing mold in that incubator and it infecting other projects unrelated (if any are in there)



As I said: it will be unused from February, so it's fine

Quote:

blindingleaf said:
the hood will be nice to have for transfers, but the incubator might do more harm then good.



Luckily we have a hood and I hadn't thought about it when I was planning! Thanks

Quote:

blindingleaf said:
don't forget that besides light, humidity, and temperature, co2 will play a role in performance.  those incubators look like there will be enough light penetration without adding more inside.

measuring the exact PPM isn't necessary to succeed, but would be interesting if u could.  the important part is recognizing that air should be moved around and replaced either by active or passive means during fruiting.



I think there's sort of a valve (the little thing on left wall), so if I find a way to move air it won't be a problem. I'm also sure that inside it's completely dark: if light goes inside it can be easily gradually avoided until I reach the desired illumination, but it looks like I have to add it.
Sadly I think I have no way to measure CO2 PPM neither control it (until reading your post I didn't even think it was so important). It's such a huge problem or I can solve it?

Thanks everyone, it means a lot for me :smile:


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OfflineBlueFire_ita
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: blindingleaf]
    #23988599 - 01/07/17 05:53 AM (7 years, 23 days ago)

Quote:

blindingleaf said:
I've never seen pans pin on agar personally, and I have tried.

I know Muda has a pic of it happening, and maybe stonesun.  so its not that it won't happen, its just that u might be waiting a long time if ur going that route.

if thats the case, u won't need to worry about co2 or humidity.  just light and temp



That's exactly what I was thinking about: I'll try to avoid too complicated things.
I could at least pot few spores in agar just turned see what happens letting them near the rest of the cultivation


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Invisibleblindingleaf
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: BlueFire_ita]
    #23988634 - 01/07/17 06:26 AM (7 years, 23 days ago)

the user "macmerdin" has a TEK for small scale pan grows basically using the ideas from PF TEK with some modifications-check that out, lots of success

maybe a clever fellow like yourself could talk the teacher into allowing that kind of approach rather than just plates.  similar in size and scale, but for sure more productive.

"pastywhyte" also has an easy agar TEK with PP5 containers that are even smaller than 1/2 pints.  an unassuming teacher wouldn't know the difference between dark colored deep agar or bulk substrates in those containers.

maybe worth a try....you know....for uhhh...s...s...ss..cience ?

cause as far as legality goes (and im no lawyer) I don't really think a judge will discriminate if there were fruits grown on an agar plate or there were fruits grown in a jar....if it fruits, its still a mushroom right?


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Invisibleberlinmyc
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: blindingleaf]
    #23989257 - 01/07/17 11:26 AM (7 years, 23 days ago)

You may need to figure out how to get some fresh air if you fruit it in there. I think it's awesome that your highschool will have hallucinogenic mushrooms growing in it. Fine work.


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Invisibleblindingleaf
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: berlinmyc]
    #23989424 - 01/07/17 12:24 PM (7 years, 23 days ago)

Oh Fuck!

I thought he said FINISHED high school :facepalm:

I'm an idiot.

Don't grow drugs in High school, just smoke them.  Growing is for adults


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlineblackout
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: BlueFire_ita]
    #23989910 - 01/07/17 03:47 PM (7 years, 23 days ago)

Quote:

BlueFire_ita said:
lucky me, of you check the controlled substances tables, you'll see that, even though they contains psilocybin, Panaeolus are perfectly growable.



can anybody else confirm if this is true? I never heard much about Italy. Wiki would suggest it is illegal but decriminalised.

Some countries had lists of species, but other laws could come into effect too.


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OfflinePinPornProducer
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: blackout]
    #23989939 - 01/07/17 03:55 PM (7 years, 23 days ago)

Pretty sure any psylocybin/psylocin containing fungi are illegal in the U.S


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Offlineenlightenment
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: PinPornProducer]
    #23989950 - 01/07/17 03:58 PM (7 years, 23 days ago)

Quote:

PinPornProducer said:
Pretty sure any psylocybin/psylocin containing fungi are illegal in the U.S




The same here in Germany. But he lives in Italy.
It might be different there. Maybe they ban the species and not the substances.


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OfflineBlueFire_ita
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: enlightenment]
    #23991688 - 01/08/17 06:57 AM (7 years, 22 days ago)

Quote:

blindingleaf said:
the user "macmerdin" has a TEK for small scale pan grows basically using the ideas from PF TEK with some modifications-check that out, lots of success

maybe a clever fellow like yourself could talk the teacher into allowing that kind of approach rather than just plates.  similar in size and scale, but for sure more productive.

"pastywhyte" also has an easy agar TEK with PP5 containers that are even smaller than 1/2 pints.  an unassuming teacher wouldn't know the difference between dark colored deep agar or bulk substrates in those containers.

maybe worth a try....you know....for uhhh...s...s...ss..cience ?

cause as far as legality goes (and im no lawyer) I don't really think a judge will discriminate if there were fruits grown on an agar plate or there were fruits grown in a jar....if it fruits, its still a mushroom right?



Are you suggesting to grow them in different ways and see the results? Maybe, if it looks possible. Are their teks in archive?


About Ialian law: yes Panaeolus are perfectly legal, then I can grow them in school (it's pretty exciting doing that, it's definitely unusual and a thing thath fits my interest).

Italian governmen website, page about illegal substances in general:
http://www.salute.gov.it/portale/temi/p2_6.jsp?id=3729&area=sostanzeStupefacenti&menu=vuoto
strictly illicit substsances:
http://www.salute.gov.it/imgs/C_17_pagineAree_3729_listaFile_itemName_0_file.pdf
"Funghi del genere Strofaria, Conocybe, Psilocybe", "Psilocibina" and "Psilocina". Nothing about Panaeolus :smile:


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Invisibleblindingleaf
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: BlueFire_ita]
    #23991698 - 01/08/17 07:10 AM (7 years, 22 days ago)

it don't change the fact ur in high school bud.

seriously, go get laid a lot and smoke some drugs.  thats what high school is for.  later on, when ur staring at a few overdrawn bank accounts and ur baby momma is hustling you, thats when u make the next move in a country where its legal to do so


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: BlueFire_ita]
    #23991705 - 01/08/17 07:24 AM (7 years, 22 days ago)

My understanding of the italian language may be lacking, but I think "Psilocibina" and "Psilocina" translate to the active chemicals psilocybin and psilocin. (which are found in the pans you want to grow.)

In the pdf you linked they list the chemical names
"Psilocibina-diidrogeno fosfato del 3-[2-(dimetilamino)etil]indol-4-olo",
"Psilocina-  3-[2-(dimetilamino)etil]indol-4-olo"

Pretty sure that means
Psilocybin -[3-(2-Dimethylaminoethyl)-1H-indol-4-yl] dihydrogen phosphate
Psilocin-4-hydroxy-N,N-dimethyltryptamine

You may want to consult a lawyer before starting this project.


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Offlineblackout
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: BlueFire_ita]
    #23991930 - 01/08/17 09:38 AM (7 years, 22 days ago)

Quote:

BlueFire_ita said:
About Ialian law: yes Panaeolus are perfectly legal,



If you think they are "perfectly legal" I would wonder what you think is a grey area.

If they really are "perfectly legal" I would expect to see them openly on sale in many of the head shops in Italy, you could take clones of them and not even bother with spores, or possibly get spores from the fresh or dry ones on sale.


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Invisibleberlinmyc
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: blindingleaf]
    #23992825 - 01/08/17 03:52 PM (7 years, 22 days ago)

Quote:

blindingleaf said:
it don't change the fact ur in high school bud.

seriously, go get laid a lot and smoke some drugs.  thats what high school is for.  later on, when ur staring at a few overdrawn bank accounts and ur baby momma is hustling you, thats when u make the next move in a country where its legal to do so



I think it's good to grow at that age. I believe Paul Stamets did and it was illegal. any involvement in illegal drugs is an active protest against unjust laws. Bluefire does your teacher know that it is psychoactive?


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OfflinePanarchist
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Re: Scholastic cultivation of Panaeolus in big "Petri dish incubator" [photo] [Re: blackout]
    #23993749 - 01/08/17 08:45 PM (7 years, 22 days ago)

i highly doubt that Italy would have laws against psilocybin/psilocin mushrooms yet somehow exempt panaeolus species from that law. it would be pretty awesome if somehow it was the case though. due to the fact that i have italian ancestry supposedly i can get fast-tracked for citizenship.


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