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Offlineblthirteenre
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Registered: 06/10/12
Posts: 430
Loc: New York
Last seen: 9 months, 17 days
BACTERIA? (PICS)
    #23987432 - 01/06/17 06:45 PM (7 years, 24 days ago)

Hello everyone,

I hope everyone has been making the most of 2017.

I want to follow up with previous posts I have made about contamination issues I've experienced. I've been doing this for about 5 years now, and frequently encounter "hurdles", this being one of them. I am experiencing what I assume to be bacteria in my jars. Here are some of them:





These are two different "strains" (indian and blue meanie (not pan cyan). You can see the difference in the myc. my guess is this is classic bacteria.

So, the plot thickens. Out of desperation, I bought an All american PC. It is very nice. After using the new PC without changing any other variables, I have noticed an obvious difference:



There does not seem to be any bacteria, although there may be some. This is G2G.

I have always PC'd 75-90 mins. I previously used a presto. The issue is that I am still suspicious that there is some bacteria. Actually, I made my first non-contaminated 18-gallon monotub successfully since early 2016.

I have always done G2G with perfect success. Master is made from MS syringe. Perhaps the atmosphere on the earth has changed and my jars are now vulnerable.

Does anyone have comments on the fact that the AA PC killed more bacteria? Aside from agar, which yes i know is the perfect solution, any ideas how the bacteria occurs and how to prevent it?


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InvisibleIntelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
Re: BACTERIA? (PICS) [Re: blthirteenre]
    #23987467 - 01/06/17 06:55 PM (7 years, 24 days ago)

From the information you just gave, its either a pc issue i.e not venting your PC before building pressure, or its " Master is made from MS syringe"

I'd bet the latter with confidence


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OfflineThedillestpickle
cultured
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Registered: 02/02/16
Posts: 1,170
Loc: Canada Flag
Last seen: 3 years, 7 months
Re: BACTERIA? (PICS) [Re: blthirteenre]
    #23987488 - 01/06/17 07:01 PM (7 years, 24 days ago)

Well every time you use a new syringe you are changing the variable of how clean the syringe is.  Also how old/viable the spores in the syringe are and how many spores will change germination time and points of germination thus changing colonization time which determines how much time bacteria have a chance to grow.

Venting is really important with steam sterilization.  Assuming that you are venting the same amount with both pressure cookers then they should do the same job at the same pressure.

Perhaps the gauges aren't right and the AA is actually reaching a higher pressure. 

The two pressure cookers are made of different materials and of different thicknesses.  Faster cooling leads to increased water condensation on the inside of the jar.  More condensation means more standing water which is a breeding ground for bacteria because they are more mobile in liquid.  So it's possible that the AA has a slower cool down which is helping prevent bacteria. 

The variables I could identify are: 
-syringe
-venting
-gauge/pressure
-cool-down time


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Offlineblthirteenre
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Registered: 06/10/12
Posts: 430
Loc: New York
Last seen: 9 months, 17 days
Re: BACTERIA? (PICS) [Re: Thedillestpickle]
    #23988922 - 01/07/17 09:32 AM (7 years, 23 days ago)

Quote:

Intelligentxfruit said:
From the information you just gave, its either a pc issue i.e not venting your PC before building pressure, or its " Master is made from MS syringe"

I'd bet the latter with confidence




Thanks, Intelligentxfruit

Quote:

Thedillestpickle said:
Well every time you use a new syringe you are changing the variable of how clean the syringe is.  Also how old/viable the spores in the syringe are and how many spores will change germination time and points of germination thus changing colonization time which determines how much time bacteria have a chance to grow.

Venting is really important with steam sterilization.  Assuming that you are venting the same amount with both pressure cookers then they should do the same job at the same pressure.

Perhaps the gauges aren't right and the AA is actually reaching a higher pressure. 

The two pressure cookers are made of different materials and of different thicknesses.  Faster cooling leads to increased water condensation on the inside of the jar.  More condensation means more standing water which is a breeding ground for bacteria because they are more mobile in liquid.  So it's possible that the AA has a slower cool down which is helping prevent bacteria. 

The variables I could identify are: 
-syringe
-venting
-gauge/pressure
-cool-down time




These variables I have not considered, so thank you very much. I will absolutely look into this.

I am making grain as we speak and I am wondering if it something to do with the way I prepare spawn, including these possibilities

Soaking the spawn for 24 hours (it went about 28 hours today)
Typically re-using the water used to soak (it smells bad but I have seen that people typically reuse it, but I am going to clean the grain and use new water)
Possibly simmering for too long (a few times the grain appeared to be over-cooked but seemed to dry out and look perfect after left in a collander)

If anything seems suspicious, please let me know.


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