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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Let's All Grow Mushrooms 2017 [Re: mushboy]
#24035886 - 01/24/17 12:07 PM (7 years, 6 days ago) |
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That's the dream! Hahaha but I do love my job (most of the time)
I'm stoked on those!! Dude did that debris jar end up doing anything?
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
#24036022 - 01/24/17 01:17 PM (7 years, 6 days ago) |
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i might birth it pf style. or 'dawwgy' bag it. or just leave it alone..


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mushboy
modboy



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Posts: 32,281
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Re: Let's All Grow Mushrooms 2017 [Re: mushboy]
#24036044 - 01/24/17 01:27 PM (7 years, 6 days ago) |
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also...
took 2 transfers from each master MS plate.
Edited by mushboy (01/24/17 01:35 PM)
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FunGuyZ
Psilly Rabbit


Registered: 12/13/16
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Re: Let's All Grow Mushrooms 2017 [Re: mushboy]
#24036075 - 01/24/17 01:47 PM (7 years, 6 days ago) |
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NIce job mushboy- you are a machine!
Personally I am still struggling with contamination in my pastyplates. I am wondering if the condensation that is built up on the inside of the plates from PCing is attracting contaminates when I open them in the SAB and then those droplets fall to the agar and cause problems. Or these droplets create too much humidity and a breeding ground. Because the culture will start to grow and as the sides start to clear is when I get problems.
Any thoughts?
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mushboy
modboy



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Re: Let's All Grow Mushrooms 2017 [Re: FunGuyZ]
#24036088 - 01/24/17 01:52 PM (7 years, 6 days ago) |
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how long are you PCin the plates?(40min 15psi) i run into few issues with my plates. every once and while ill get something but its rare. and im so sloppy with it tho.
make sure hands never go over a open plate, i drench my gloves in iso, uhh flame the blade like crazy??
i flick the glads really hard with my finger. condensation goes away after a day or so. the myc will consume the water. it shouldnt pool or anything.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Let's All Grow Mushrooms 2017 [Re: FunGuyZ]
#24036092 - 01/24/17 01:53 PM (7 years, 6 days ago) |
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Wheres the contamination coming from? If it's coming from your transfered you just have more cleaning to do. If they're at the edges of your plates than you need to tighten up sterile tech
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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FunGuyZ
Psilly Rabbit


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Re: Let's All Grow Mushrooms 2017 [Re: mushboy]
#24036102 - 01/24/17 01:57 PM (7 years, 6 days ago) |
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45m at 15psi.
My hands did go over the plates so next time I will make sure not to do that. I do flame like crazy- I wait until I feel heat from the part I am holding. I was getting some pooling water but it has since been eaten.
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FunGuyZ
Psilly Rabbit


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Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
#24036111 - 01/24/17 01:59 PM (7 years, 6 days ago) |
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Well the ones I am trying to clean up I am not worried about- I am expecting that to take some time. Mostly concerned about my newer plates from the most recent clones that I took that I have yet to transfer. 1st set of clones I have already transferred. Going to make some more today and transfer both sets.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
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Re: Let's All Grow Mushrooms 2017 [Re: FunGuyZ]
#24036123 - 01/24/17 02:03 PM (7 years, 6 days ago) |
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also never touch the rims or the bottom of the lids.
my right hand holds the knife and a lid. the left holds the plate and i rotate the plate around the blade to make my cuts.
i like to do the work in the upper part of the box close to the plastic between the arm holes
i never do cuts from a plate thats sitting on the floor of the box.
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FunGuyZ
Psilly Rabbit


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Re: Let's All Grow Mushrooms 2017 [Re: mushboy]
#24036136 - 01/24/17 02:06 PM (7 years, 6 days ago) |
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Also, do you guys put down towels or no? And where do you place your plates in the SAB?
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mushboy
modboy



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Re: Let's All Grow Mushrooms 2017 [Re: FunGuyZ]
#24036142 - 01/24/17 02:09 PM (7 years, 6 days ago) |
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yah i use a towel.
i just put stuff inside?
i like to do 3-4 small quick batches in the SAB instead of 1-2 big half hour project.
quicker you work the better.
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FunGuyZ
Psilly Rabbit


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Re: Let's All Grow Mushrooms 2017 [Re: mushboy]
#24036153 - 01/24/17 02:12 PM (7 years, 6 days ago) |
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Well I guess what I meant is if you place your towel down and you spray it with water/soap and it's designed to catch contams like the rest of the tubs walls then where do you place your plates inside? Because won't the bottoms of the plates be dirty as you're working with them?
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
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Re: Let's All Grow Mushrooms 2017 [Re: FunGuyZ]
#24036159 - 01/24/17 02:14 PM (7 years, 6 days ago) |
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Everything outside the plate is dirty. That's why we only allow flame sterilized tools to hover over our open media.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
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Re: Let's All Grow Mushrooms 2017 [Re: Dactylium]
#24036208 - 01/24/17 02:29 PM (7 years, 6 days ago) |
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I just use an upside down tote. The lid is the bottom of my SAB. I don't spray the bottom. I like it dry or shit starts slipping and sliding around like Richard Simmons WHOOOAAHHH!
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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FunGuyZ
Psilly Rabbit


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Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
#24036212 - 01/24/17 02:30 PM (7 years, 6 days ago) |
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Thanks for the feedback fellas
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Dactylium
Don't touch me I'm sterile


Registered: 08/12/16
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Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
#24036215 - 01/24/17 02:31 PM (7 years, 6 days ago) |
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Okay okay we get it you have the hands of a neurosurgeon
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Kenetic
Nam Sayin



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Re: Let's All Grow Mushrooms 2017 [Re: Dactylium]
#24036382 - 01/24/17 03:30 PM (7 years, 6 days ago) |
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I just use the lid as the bottom and wipe down with alcohol. I put all plates near the back and organize them in such a way that I dont reach over them. Everything I put in the sab I wipe thoroughly with iso, and wipe down again before I open. I make sure not to breath near the arm holes or on my arms or the scalpel. Other than that it just takes some practice.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Intelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
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Quote:
tryptkaloids said:
Quote:
Corona11 said:
Quote:
Intelligentxfruit said: Yeah corona, the middle growth looks good but holy shit dude wrap the plates from now on or put em in a ziplock lmao
That is a good post tool, definitely educated me
-blah-
I thought you only needed to wrap them ehen it was petri dishes.
it kinda looks like a petri. take a transfer from the middle colony
My bad dude they looked like petris at a glance but now that you say their jars the glass on the bottom is obvious. you shouldn't have to wrap them but those are definitely contamination around the edges. Are you tightening with the rubber side down? and do you wipe the top of you jar lids with an iso towel before screwing on and pcing?
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Corona11
The New Guy



Registered: 12/09/16
Posts: 151
Last seen: 6 years, 2 days
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My bad dude they looked like petris at a glance but now that you say their jars the glass on the bottom is obvious. you shouldn't have to wrap them but those are definitely contamination around the edges. Are you tightening with the rubber side down? and do you wipe the top of you jar lids with an iso towel before screwing on and pcing?
Its all good, I am using the moded lids, white lids with polyfil and RTV. I just started wiping my jars before PC, wasnt doing it before. But like always when I reread TEKs and I see things I am not doing and have to address it to make it a habit.
Man I wanted to do some stinking transfers tonight but its late now, had an assignment do for school and it took WAY longer than I thought it would have. Maybe tomorrow I'll get some fresh agar poured. Seeing mush's new plates makes me jelly. hah
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SloppyJoseph
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Re: Let's All Grow Mushrooms 2017 [Re: Corona11]
#24037141 - 01/24/17 08:09 PM (7 years, 6 days ago) |
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I don't use a towel and I use the table I'm working on as the bottom of my SAB. I don't like how the lids have all the random ridges and protrusions. I clean it with a ton of iso and then lay the SAB on it still wet. So by the time I get in there it's dry and also clean
-------------------- AMU Q&A
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