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InvisiblemushboyMDiscord
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Re: Let's All Grow Mushrooms 2017 [Re: Corona11]
    #24034437 - 01/23/17 08:13 PM (7 years, 7 days ago)

looks fine i guess.

take transfers from the most uniform looking growth. dont worry if you see no rhizo. clean strong growth is way more important.


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InvisibleComebackKid
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Re: Let's All Grow Mushrooms 2017 [Re: mushboy] * 1
    #24034480 - 01/23/17 08:30 PM (7 years, 7 days ago)

I like the growth in the middle


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleTookitooki
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Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
    #24034513 - 01/23/17 08:47 PM (7 years, 7 days ago)

Quote:

Intelligentxfruit said:
thats what they were debating lol



 

I went back and edited one of my posts with a quote from bod, he was really laying the knowledge on this subject in pic of the day thread.  So I put the proper info in my post. 

For the record, bod is the man!!

bodhisatta said:
Evaporation certainty helps it's not the reason it works better though. 70 works at a fundamental level differently than 90+%
College professors have been known to be dumbasses...

Higher alcohol concentrations, like 91%-99%, coagulates protein on contact, creating a "shell" around the entire bacterial organism. After that shell is made, the alcohol CANNOT go through that shell to further coagulate the rest of the bacterial organism and its organelles. This will render the bacteria, not dead, but inactive. If given favorable conditions, it can start to function once more. Now if you use 70% alcohol (which has the highest osmotic pressure of alcohol/water mixtures), it will coagulate the protein but it will do so at a much slower rate, giving it the opportunity to penetrate fully. Since it does this slowly, nothing can coagulate in time to stop it which ends up leaving the bacterial organism dead.


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InvisibleIntelligentxfruit
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Re: Let's All Grow Mushrooms 2017 [Re: Tookitooki]
    #24034549 - 01/23/17 09:01 PM (7 years, 7 days ago)

Yeah corona, the middle growth looks good but holy shit dude wrap the plates from now on or put em in a ziplock lmao

That is a good post tool, definitely educated me:thumbup:

-blah-


Edited by Intelligentxfruit (01/23/17 11:22 PM)


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OfflineCrispykoot
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Re: Let's All Grow Mushrooms 2017 [Re: bodhisatta]
    #24034616 - 01/23/17 09:33 PM (7 years, 6 days ago)

Quote:

Tookitooki said:
Quote:

Intelligentxfruit said:
thats what they were debating lol



 

I went back and edited one of my posts with a quote from bod, he was really laying the knowledge on this subject in pic of the day thread.  So I put the proper info in my post. 

For the record, bod is the man!!

bodhisatta said:
Evaporation certainty helps it's not the reason it works better though. 70 works at a fundamental level differently than 90 %
College professors have been known to be dumbasses...

Higher alcohol concentrations, like 91%-99%, coagulates protein on contact, creating a "shell" around the entire bacterial organism. After that shell is made, the alcohol CANNOT go through that shell to further coagulate the rest of the bacterial organism and its organelles. This will render the bacteria, not dead, but inactive. If given favorable conditions, it can start to function once more. Now if you use 70% alcohol (which has the highest osmotic pressure of alcohol/water mixtures), it will coagulate the protein but it will do so at a much slower rate, giving it the opportunity to penetrate fully. Since it does this slowly, nothing can coagulate in time to stop it which ends up leaving the bacterial organism dead.





Doesn't ethyl alcohol have the highest pressure? Also, Iso can be used in the 70-90% range and be effective as far as I have read.
Quote:

bodhisatta said:
Evaporation has nothing to do with the effectiveness. 70 kills better than 91. Evaporation is a secondary benefit.

91 denatures cell wall protein and will disable but not necessarily kill a bacterium

70 will be more allowed through the cell wall and then denatures internal proteins and kills cells.





Hey Bod. Just to try to get clear on this...Isn't Ethyl alcohol most effective in the 70% range? Also isn't the Ethyl alcohol the one with the highest pressure? From what I read on CDC's site they say 70-90% Isopropyl is effective against bacteria.

https://www.cdc.gov/hicpac/disinfection_sterilization/6_0disinfection.html


--------------------






:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:


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OfflineCorona11
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Re: Let's All Grow Mushrooms 2017 [Re: Intelligentxfruit]
    #24035150 - 01/24/17 05:02 AM (7 years, 6 days ago)

Quote:

Intelligentxfruit said:
Yeah corona, the middle growth looks good but holy shit dude wrap the plates from now on or put em in a ziplock lmao

That is a good post tool, definitely educated me:thumbup:

-blah-





I thought you only needed to wrap them ehen it was petri dishes.


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Offlinetryptkaloids
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Re: Let's All Grow Mushrooms 2017 [Re: Corona11]
    #24035342 - 01/24/17 07:57 AM (7 years, 6 days ago)

Quote:

Corona11 said:
Quote:

Intelligentxfruit said:
Yeah corona, the middle growth looks good but holy shit dude wrap the plates from now on or put em in a ziplock lmao

That is a good post tool, definitely educated me:thumbup:

-blah-





I thought you only needed to wrap them ehen it was petri dishes.



it kinda looks like a petri.  take a transfer from the middle colony


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineCorona11
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Re: Let's All Grow Mushrooms 2017 [Re: tryptkaloids]
    #24035443 - 01/24/17 09:05 AM (7 years, 6 days ago)

I plan on taking a few transfers tonight if I get all my homework done hahah. Screw college, naw im JK.
Lol no, not petris.  Those are widemouth half pints, rtv and polyfill on the lids.


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Invisiblewolfedawwg
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Re: Let's All Grow Mushrooms 2017 [Re: Corona11]
    #24035613 - 01/24/17 10:07 AM (7 years, 6 days ago)

I'll be doing my 2nd and hopefully final transfers tonight on both clone and spores.

The candidates...

1st spore transfers


1st clone transfers


--------------------
:jah:Easy as Fuck Isopropyl Hash Oil:jah:


Psilocybe quebecensis



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InvisiblemushboyMDiscord
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Re: Let's All Grow Mushrooms 2017 [Re: wolfedawwg]
    #24035616 - 01/24/17 10:08 AM (7 years, 6 days ago)

nice.

im doing transfers soon myself :wink:

also gotta spawn some aa+ and mexican.. busy busy busy.


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OfflineKenetic
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Re: Let's All Grow Mushrooms 2017 [Re: mushboy]
    #24035619 - 01/24/17 10:11 AM (7 years, 6 days ago)

Awesome.  Im probably a week away from spawning


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleAneres
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Re: Let's All Grow Mushrooms 2017 [Re: Kenetic]
    #24035632 - 01/24/17 10:15 AM (7 years, 6 days ago)

Quote:

kenetic said:
Awesome.  Im probably a week away from spawning




Too awesome!


I'm on my first transfer, lookit 'em go!


--------------------
Have fun on harvest day!


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OfflineKenetic
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Re: Let's All Grow Mushrooms 2017 [Re: Aneres]
    #24035635 - 01/24/17 10:16 AM (7 years, 6 days ago)

Nice!


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisiblemushboyMDiscord
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Re: Let's All Grow Mushrooms 2017 [Re: Kenetic]
    #24035652 - 01/24/17 10:22 AM (7 years, 6 days ago)

:eek:

awesome


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Invisiblewolfedawwg
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Re: Let's All Grow Mushrooms 2017 [Re: mushboy]
    #24035665 - 01/24/17 10:26 AM (7 years, 6 days ago)

I might go for a walk today and collect enough rabbit and deer shit to do a single bottle grow, just for shits and giggles.
There are tonnes of rabbits up behind my place and these 3 have been coming out to feed in my back yard regularly...


--------------------
:jah:Easy as Fuck Isopropyl Hash Oil:jah:


Psilocybe quebecensis



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OfflineTiamo
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Re: Let's All Grow Mushrooms 2017 [Re: wolfedawwg]
    #24035683 - 01/24/17 10:33 AM (7 years, 6 days ago)

Things are looking up with my pasty plates. We have germination and no sign of contamination on any of them. :rockon:


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisibleComebackKid
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Re: Let's All Grow Mushrooms 2017 [Re: wolfedawwg]
    #24035684 - 01/24/17 10:33 AM (7 years, 6 days ago)

Damn you all! I still waiting on plates to germinate!! :lol:
Tbh I've been crazy busy lately working late and early shifts.. I would have nocked a few more up by now but sleeeeeep..
All my cultures that I have on the go are grown out and sucking the food colouring out of my agar. I may get around to transfering tomorrow evening. Hopefully


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineTiamo
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Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
    #24035685 - 01/24/17 10:33 AM (7 years, 6 days ago)

Whoa we just posted on the exact same second


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisibleComebackKid
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Re: Let's All Grow Mushrooms 2017 [Re: Tiamo]
    #24035699 - 01/24/17 10:38 AM (7 years, 6 days ago)

Weird it didn't even warn me that someone else posted.


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisiblemushboyMDiscord
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Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
    #24035713 - 01/24/17 10:48 AM (7 years, 6 days ago)

who needs a job. just grow shrooms all day.
Quote:

ComebackKid said:
Damn you all! I still waiting on plates to germinate!! :lol:
Tbh I've been crazy busy lately working late and early shifts.. I would have nocked a few more up by now but sleeeeeep..
All my cultures that I have on the go are grown out and sucking the food colouring out of my agar. I may get around to transfering tomorrow evening. Hopefully





i got a certain aa+ recovering from shake before spawning. they are growing in your honor.


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