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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
    #24025762 - 01/20/17 02:13 PM (7 years, 10 days ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 01:29 PM)


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OfflineColonel
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Registered: 11/04/16
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Re: Let's All Grow Mushrooms 2017 [Re: amidogen]
    #24025871 - 01/20/17 02:58 PM (7 years, 10 days ago)

Have something strange happening in a couple of my plates, check it out.


--------------------

P. Ovoideocystidiata                          P. Cyanescens                                P. Northwest
Colonel's Roatan/Honduras LC
Thai Lipa Yai Monotub Grow


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InvisibleComebackKid
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Registered: 05/27/16
Posts: 3,951
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Re: Let's All Grow Mushrooms 2017 [Re: Colonel]
    #24025935 - 01/20/17 03:23 PM (7 years, 10 days ago)

That is strange
Bacteria?


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineKenetic
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Registered: 08/24/14
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Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
    #24025964 - 01/20/17 03:33 PM (7 years, 10 days ago)

wierd


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineColonel
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Re: Let's All Grow Mushrooms 2017 [Re: Kenetic]
    #24026017 - 01/20/17 03:49 PM (7 years, 10 days ago)

It looks even more odd in person, the strands are quite thick and very white reaching over a thin cloudy layer.
The mycelium seems to be sinking into the agar as well seems most definitely like bacterial growth to me. :shocked: Maybe the myc is trying to fight it.


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Offlineaxis_tech
Rollin' Stoned


Registered: 02/27/05
Posts: 294
Last seen: 4 years, 9 months
Re: Let's All Grow Mushrooms 2017 [Re: Colonel]
    #24026071 - 01/20/17 04:01 PM (7 years, 10 days ago)

I had something like that happen to me with some Texan monocoltures I was getting ready. When I was done with transfers I opened it up and there was no smell to it at all and it had the exact same texture/ firmness of soft agar. When I was watching it it didn't seem like the mycelium had much trouble growing over it.


--------------------
takes a token' and keeps on smoken'


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OfflineDactylium
Don't touch me I'm sterile


Registered: 08/12/16
Posts: 696
Last seen: 10 months, 13 days
Re: Let's All Grow Mushrooms 2017 [Re: Colonel]
    #24026176 - 01/20/17 04:34 PM (7 years, 10 days ago)

Looks like it etched some runes into your agar


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InvisibleTookitooki
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Registered: 07/28/16
Posts: 1,157
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Re: Let's All Grow Mushrooms 2017 [Re: Dactylium]
    #24026240 - 01/20/17 04:59 PM (7 years, 10 days ago)

It would be neat to see from above.


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OfflineColonel
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Re: Let's All Grow Mushrooms 2017 [Re: Tookitooki]
    #24026264 - 01/20/17 05:02 PM (7 years, 10 days ago)

Quote:

Tookitooki said:
It would be neat to see from above.




Wish I could see through my Pasty Plates better in photos, but I'll keep an eye on this. :justdontknow:


--------------------

P. Ovoideocystidiata                          P. Cyanescens                                P. Northwest
Colonel's Roatan/Honduras LC
Thai Lipa Yai Monotub Grow


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OfflinecronicrFacebook
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Re: Let's All Grow Mushrooms 2017 [Re: ComebackKid]
    #24026287 - 01/20/17 05:10 PM (7 years, 10 days ago)

Quote:

ComebackKid said:
That is strange
Bacteria?



I'd guess yes


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineKenetic
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Re: Let's All Grow Mushrooms 2017 [Re: cronicr] * 1
    #24026591 - 01/20/17 07:23 PM (7 years, 10 days ago)

Got a nice update for you fellas.  Was able to clean up a heavily bacterial set of plates.

From this:






To this:




See the full story here:

First transfer from contaminates


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineTheDuder
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Re: Let's All Grow Mushrooms 2017 [Re: Kenetic]
    #24026695 - 01/20/17 08:18 PM (7 years, 10 days ago)

Got some Albino A+ germinated in 5 days. excited to get to work! :rockon:


--------------------

|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|


|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|


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OfflineColonel
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Re: Let's All Grow Mushrooms 2017 [Re: TheDuder]
    #24026906 - 01/20/17 09:58 PM (7 years, 10 days ago)

Nice work boys


--------------------

P. Ovoideocystidiata                          P. Cyanescens                                P. Northwest
Colonel's Roatan/Honduras LC
Thai Lipa Yai Monotub Grow


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InvisibleTookitooki
Mycological Fabricator
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Registered: 07/28/16
Posts: 1,157
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Re: Let's All Grow Mushrooms 2017 [Re: Colonel]
    #24027481 - 01/21/17 07:39 AM (7 years, 9 days ago)

I still have a pile of plates that have not geminated.  But in three days my b+ germinated, and my pan cambos are killing it.  But nothing from 5 other syringes. 

I'm 114 pages in to the "Official Copelandia/Panaeolus Thread". And I've found a lot of good info on germinating stubborn spores.  You guys up for a good in-depth discussion on this subject? Afterward I'll sort and compile all the info and put it to its own thread.


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Let's All Grow Mushrooms 2017 [Re: Tookitooki]
    #24027507 - 01/21/17 08:00 AM (7 years, 9 days ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 01:29 PM)


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InvisibleJosex
#cheat_code
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Registered: 11/13/15
Posts: 8,995
Loc: Flag
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Re: Let's All Grow Mushrooms 2017 [Re: Tookitooki]
    #24027514 - 01/21/17 08:04 AM (7 years, 9 days ago)

Quote:

Tookitooki said:
I still have a pile of plates that have not geminated.  But in three days my b+ germinated, and my pan cambos are killing it.  But nothing from 5 other syringes. 

I'm 114 pages in to the "Official Copelandia/Panaeolus Thread". And I've found a lot of good info on germinating stubborn spores.  You guys up for a good in-depth discussion on this subject? Afterward I'll sort and compile all the info and put it to its own thread.




Wouldn't be too bad to know those tricks! I have a pan cyan print that so far has shown to be non-viable, I don't even know how old it is. The other day I nocced some plates with it and poured hot agar over them (not too hot)... two weeks and still no growth.

Can you link to the posts in that thread where they were giving advice about germinating stubborn spores?


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InvisibleTookitooki
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Registered: 07/28/16
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Re: Let's All Grow Mushrooms 2017 [Re: Josex]
    #24027528 - 01/21/17 08:14 AM (7 years, 9 days ago)

Lol your best bet is to take the time and read the whole thing.  Not just for the stubborn spores, but just the amount of knowledge you will gain.  You won't have many questions left to ask once you read it. Once in out of work I'll make a big post on the stubborn spores give me two hours.


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InvisiblemushboyMDiscord
modboy
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Re: Let's All Grow Mushrooms 2017 [Re: Tookitooki]
    #24027532 - 01/21/17 08:18 AM (7 years, 9 days ago)

SHAMELESS SELF PLUGGING!!

check out my journal for pan grow. its part of my LAGM2017 but i did a separate thread.


similar to tookitooki my pan cambo grow is going nicely.

however the pancyan aussies are fucked. need to throw more shrimp on the barbie mate(spores to agar)


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InvisibleJosex
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Re: Let's All Grow Mushrooms 2017 [Re: Tookitooki]
    #24027533 - 01/21/17 08:18 AM (7 years, 9 days ago)

Will do :thumbup: and write the fucking post :hehehe:


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InvisibleTookitooki
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Posts: 1,157
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Re: Let's All Grow Mushrooms 2017 [Re: Tookitooki]
    #24027825 - 01/21/17 10:30 AM (7 years, 9 days ago)

Agar problems: germination and contamination, how to deal with it.

Options for spore germination when they just don't want to:

Liquid culture
Softer agar
Innoculating when agar is not fully set
Hot pouring
Lower nutrient agar

Not sure how to word it, but the differences in the ways we inoculate our plates from SPORES

inoculation loop
  Indentation
  Zig zag

Drop from syringe:
  Roll the drop around
  Let it sit
  Make indentation for drop
  Put drop on q tip and then plate

Contamination clean up

Streaking

Agar sandwich

Anti bacterial agar

Lower nutrient agar


If I missed anything let me know.  Which methods do you use, when and why?


Edited by Tookitooki (01/21/17 10:41 AM)


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