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Loveshroomz777


Registered: 11/19/16
Posts: 616
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First grow
#23985301 - 01/05/17 09:53 PM (7 years, 25 days ago) |
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So I'm going to be doing a pf tek then breaking up the cakes into a monotub I will be doing coir and vermiculite as my substrate does this method usually give weak fruits? I read somewhere that pf tek usually gives weak fruits is this true? Thanks guys
-------------------- PEACE, LOVE AND PSYCHEDELICS
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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You're asking a lot of questions you can search for.
If you're not doing grains then just let cakes be cakes. Learn agar and do grains while you wait on your cakes to finish. It will never hurt to have a SGFC around to test genetics. In 2 weeks make new cakes so you'll have a couple irons in the fire if something fucks. I got a syringe from a member lost 12 cakes got pretty pissed, but still had 18 more and 12 in the chamber.
Breaking up colonized cakes or shredding them is just asking for a tub to fail. It harms the network so much and adds contam vectors. The end result isn't always fantastic either.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First grow [Re: Adden]
#23985332 - 01/05/17 10:04 PM (7 years, 25 days ago) |
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Quote:
Adden said: Breaking up colonized cakes or shredding them is just asking for a tub to fail. It harms the network so much and adds contam vectors. The end result isn't always fantastic either.
i dont want to argue the about potential damage to the network as that makes sense i guess but i used to have better then cake results with cakes in simple trays mixed with cv.
to say its asking for a fail is a stretch. HOWEVER, its not nearly good as agar>grains>bulk. but its a start.
also OP, ive had extremely potent cubes grown off brf. also had the weakest from hpoo once. i guess its all in the jeans.
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Adden

Registered: 06/04/03
Posts: 39,201
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Re: First grow [Re: mushboy] 1
#23985337 - 01/05/17 10:09 PM (7 years, 25 days ago) |
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If you're making cakes just fruit the cakes, and do agar/grains/bulk. Practice clean technique in a still air box. Learn how to clean up a print. Otherwise you'll be making MS cakes and trays forever and know nothing.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First grow [Re: Adden]
#23985346 - 01/05/17 10:12 PM (7 years, 25 days ago) |
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Quote:
Adden said: Otherwise you'll be making MS cakes and trays forever and know nothing.
that might be someones goal ya know. making cakes and trays is fine. not everyone needs to go from 1st grow to agar in one fluid leap.
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ultramanjay
Jahman



Registered: 09/19/16
Posts: 60
Loc: Pearl of the Orient Seas
Last seen: 3 years, 1 month
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Re: First grow [Re: mushboy]
#23985489 - 01/05/17 11:26 PM (7 years, 25 days ago) |
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How do you clean a print? And how does MS printing happen when your only getting it from a single cap?
-------------------- “In biology, nothing is clear, everything is too complicated, everything is a mess, and just when you think you understand something, you peel off a layer and find deeper complications beneath. Nature is anything but simple.” ― Richard Preston
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
Posts: 1,811
Loc: England
Last seen: 5 years, 11 months
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Quote:
ultramanjay said: How do you clean a print? And how does MS printing happen when your only getting it from a single cap?
By working with agar! Type pastywhyte into the search engine to find out an easy method.
As for the spore question, I honestly don't know. I'll let someone more knowledgeable answer so I don't misinform you.
-------------------- Ask and ye shall receive; Seek and ye shall find.
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ultramanjay
Jahman



Registered: 09/19/16
Posts: 60
Loc: Pearl of the Orient Seas
Last seen: 3 years, 1 month
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Oh agar. I havent really tried that before. Im on my first time doing the PF tek. I know that batch is ruined. The print that i used is from a shroom that i found in the wild. Sigh. But im beginning to be addicted to cultivating.
-------------------- “In biology, nothing is clear, everything is too complicated, everything is a mess, and just when you think you understand something, you peel off a layer and find deeper complications beneath. Nature is anything but simple.” ― Richard Preston
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
Posts: 1,811
Loc: England
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It's good to try new things, and it's a lot more productive and rewarding to work with agar than something like the PF Tek (you can do PF a few times just to get a feel for it of course)
It also means if your current PFs give you fruit, you can clone that fruit on agar
-------------------- Ask and ye shall receive; Seek and ye shall find.
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ultramanjay
Jahman



Registered: 09/19/16
Posts: 60
Loc: Pearl of the Orient Seas
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Doing it on agar looks pretty scientisty! I think thats awesome! Im building my mushroom lab right now.
-------------------- “In biology, nothing is clear, everything is too complicated, everything is a mess, and just when you think you understand something, you peel off a layer and find deeper complications beneath. Nature is anything but simple.” ― Richard Preston
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
Posts: 1,811
Loc: England
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As scientisty as mixing up powders with water, putting em in polypropylene containers, then pressure cooking the containers. You then have functioning agar dishes with no conspicuous waste that comes with using disposable petris.
-------------------- Ask and ye shall receive; Seek and ye shall find.
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ultramanjay
Jahman



Registered: 09/19/16
Posts: 60
Loc: Pearl of the Orient Seas
Last seen: 3 years, 1 month
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Is polypropylene the one walt told jesse to buy for dissolving emilio's corpse?
-------------------- “In biology, nothing is clear, everything is too complicated, everything is a mess, and just when you think you understand something, you peel off a layer and find deeper complications beneath. Nature is anything but simple.” ― Richard Preston
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Loveshroomz777


Registered: 11/19/16
Posts: 616
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How is breaking up Cakes and mixing them into a mono tub asking for contamination I thought people do this all the time I forgot what the process is called
-------------------- PEACE, LOVE AND PSYCHEDELICS
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,030
Loc: Temple of Time
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pfcakes aren't 100% clean. although i've made some decent trays with them. XD
-------------------- Song Of Healing
Updated Pf Tek Guide Ziran's Teks AMU Q&A Thread The Chinese word for nature is zìrán and it means that of which is of itself.

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Loveshroomz777


Registered: 11/19/16
Posts: 616
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Re: First grow [Re: Ziran]
#23985641 - 01/06/17 01:24 AM (7 years, 24 days ago) |
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So do you recommend just fruiting the cakes instead of breaking them up and mixing it with coir and vermiculite in a monotub I want to increase my yield
-------------------- PEACE, LOVE AND PSYCHEDELICS
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,030
Loc: Temple of Time
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cakes are guaranteed. you win some you lose some with trays. that's how it goes with pf tek spawned trays. if you do spawn them don't dick with them. that's my only advice. other than that i've never done a monotub spawned with PFtek cakes so I can't give you advice there.
-------------------- Song Of Healing
Updated Pf Tek Guide Ziran's Teks AMU Q&A Thread The Chinese word for nature is zìrán and it means that of which is of itself.

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RealityCzech
Entropy enthusiast


Registered: 12/29/16
Posts: 90
Last seen: 6 years, 3 months
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Quote:
ultramanjay said: How do you clean a print? And how does MS printing happen when your only getting it from a single cap?
As far as the MS question goes...
Think of the mushroom as a sex organ. The entire mushroom is made up of identical dikaryotic cells. Spores are monokaryotes, so when they are produced the genetics from the dikaryotic tissue gets shuffled. A fair analogy is that the spores are all siblings... same parent genetics, just recombined in a bunch of different ways.
I know that I'm really oversimplifying this, but hopefully you have an idea of how this goes.
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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You're really oversimplifying it because you're wrong. Even a clone isn't a perfect set of the same cells. Latent genetics sneak in through horizontal gene transfer. It's why if you run the same isolate it can shit the bed after so many runs, or why you get a random weird looking mushroom in your clone set and people write it off as the substrate or some other factor. They are constantly changing their genetics no matter what you do.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 01:08 PM)
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RealityCzech
Entropy enthusiast


Registered: 12/29/16
Posts: 90
Last seen: 6 years, 3 months
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Re: First grow [Re: Adden]
#23993861 - 01/08/17 09:29 PM (7 years, 22 days ago) |
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Quote:
Adden said: You're really oversimplifying it because you're wrong. Even a clone isn't a perfect set of the same cells. Latent genetics sneak in through horizontal gene transfer. It's why if you run the same isolate it can shit the bed after so many runs, or why you get a random weird looking mushroom in your clone set and people write it off as the substrate or some other factor. They are constantly changing their genetics no matter what you do.
You're absolutely right, of course. My point is that there is quite a bit of genetic variation between spores from a single fruiting body. I've said it before and I'll say it again... Thank you, sexual reproduction!
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ultramanjay
Jahman



Registered: 09/19/16
Posts: 60
Loc: Pearl of the Orient Seas
Last seen: 3 years, 1 month
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Quote:
RealityCzech said:
Quote:
Adden said: You're really oversimplifying it because you're wrong. Even a clone isn't a perfect set of the same cells. Latent genetics sneak in through horizontal gene transfer. It's why if you run the same isolate it can shit the bed after so many runs, or why you get a random weird looking mushroom in your clone set and people write it off as the substrate or some other factor. They are constantly changing their genetics no matter what you do.
You're absolutely right, of course. My point is that there is quite a bit of genetic variation between spores from a single fruiting body. I've said it before and I'll say it again... Thank you, sexual reproduction!
Thanks guys, at least it gave me an idea of how it looks like.
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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What what looks like? A nice isolate? Tissue on agar? A cell?
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ultramanjay
Jahman



Registered: 09/19/16
Posts: 60
Loc: Pearl of the Orient Seas
Last seen: 3 years, 1 month
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Re: First grow [Re: Adden]
#23994233 - 01/09/17 01:03 AM (7 years, 21 days ago) |
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Quote:
Adden said: What what looks like? A nice isolate? Tissue on agar? A cell?
A concept map of multi spore?
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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I guess think of it as us being 8 billion asexual creatures, able to regenerate and create more organisms like starfish or flatworms, or create copies of ourselves, and able to mate with other people with genetic and recessive genes.. if I lopped off my arm and it turned into another me, it wouldn't be an exact genetic copy after a few times doing that. Sort of.
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: First grow [Re: Adden]
#23994239 - 01/09/17 01:10 AM (7 years, 21 days ago) |
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Here.. read this.. first 5 chapters are free online but the book is worth buying.
http://www.mycolog.com/fifthtoc.html
The Fifth Kingdom ... Bryce Kendrick
https://www.amazon.com/Fifth-Kingdom-Bryce-Kendrick/dp/1585100226
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ultramanjay
Jahman



Registered: 09/19/16
Posts: 60
Loc: Pearl of the Orient Seas
Last seen: 3 years, 1 month
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Re: First grow [Re: Adden]
#23994246 - 01/09/17 01:22 AM (7 years, 21 days ago) |
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Quote:
Adden said: Here.. read this.. first 5 chapters are free online but the book is worth buying.
http://www.mycolog.com/fifthtoc.html
The Fifth Kingdom ... Bryce Kendrick
https://www.amazon.com/Fifth-Kingdom-Bryce-Kendrick/dp/1585100226
right on man
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Yeah man sorry I can't give a proper analogy.. they're an organism all their own.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,363
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Quote:
amidogen said:
Quote:
ultramanjay said: Is polypropylene the one walt told jesse to buy for dissolving emilio's corpse?

No. Polypropylene is plastic. Like the plastic that makes up the Glad mini rounds lots of folks on here love to make no-pour agar in, and that you should be able to find at your local grocer.
For reference, Walt told Jesse to use hydrofluoric acid, which in reality is an awful idea and wouldn't work too well. Ol' Walt should have gone for fluoroantimonic acid - it's a superacid so strong they have to store it in containers made of Teflon.
I think ultramanjay is getting confused with the container that Walt said to use, as HF Acid doesnt burn thru some plastics, like polypylene perhaps.
Didnt he tell Jesse to check for plastic containers for the right plastic?
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
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ultramanjay
Jahman



Registered: 09/19/16
Posts: 60
Loc: Pearl of the Orient Seas
Last seen: 3 years, 1 month
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Quote:
LogicaL Chaos said:
Quote:
amidogen said:
Quote:
ultramanjay said: Is polypropylene the one walt told jesse to buy for dissolving emilio's corpse?

No. Polypropylene is plastic. Like the plastic that makes up the Glad mini rounds lots of folks on here love to make no-pour agar in, and that you should be able to find at your local grocer.
For reference, Walt told Jesse to use hydrofluoric acid, which in reality is an awful idea and wouldn't work too well. Ol' Walt should have gone for fluoroantimonic acid - it's a superacid so strong they have to store it in containers made of Teflon.
I think ultramanjay is getting confused with the container that Walt said to use, as HF Acid doesnt burn thru some plastics, like polypylene perhaps.
Didnt he tell Jesse to check for plastic containers for the right plastic?
Sorry for leading this thread off topic guys haha nevermind about that
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