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OfflineKulkulcan
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Registered: 05/04/16
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keep PF spawn strong until G2G?
    #23983610 - 01/05/17 11:02 AM (7 years, 25 days ago)

Suppose you had some PF jars going that were only about 50% colonized, but when they DID get to be 100% colonized, you wanted your next step to be G2G, but you didn't think you could get to that right away.

Would it be better to keep the PF jars at around 15 deg C (59 F) to slow them down, or to keep going at around 24 deg C (75 F) until they were 100% colonized, and then put them in the fridge for just a week or two?

BTW, let's say that contams hadn't been a problem, and PF spawn looked rhyzo & strong.


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Offlinepblcenmy1
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan]
    #23983676 - 01/05/17 11:30 AM (7 years, 25 days ago)

Wait for full colonization. That way when things warm up again theres no suprises after transfer. Good Luck!


--------------------
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Invisibler.lutece
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Re: keep PF spawn strong until G2G? [Re: pblcenmy1]
    #23983696 - 01/05/17 11:39 AM (7 years, 25 days ago)

Definitely 100% colonized before refrigeration. :thumbup:


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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OfflineKulkulcan
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Re: keep PF spawn strong until G2G? [Re: r.lutece]
    #23983747 - 01/05/17 11:57 AM (7 years, 25 days ago)

Thanks!


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OfflinecronicrFacebook
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan]
    #23983964 - 01/05/17 01:36 PM (7 years, 25 days ago)

cakes themselves may be clean but your dry layer may not be they are always a risk, if you are confident they are clan you could get myc water from them:smile:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

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Offlineunlimitedmaster
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Re: keep PF spawn strong until G2G? [Re: cronicr]
    #23983986 - 01/05/17 01:45 PM (7 years, 25 days ago)

You can remove the dry layer :shrug:


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OfflineKenetic
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Re: keep PF spawn strong until G2G? [Re: unlimitedmaster]
    #23984342 - 01/05/17 04:09 PM (7 years, 25 days ago)

And in the process contaminate the "spawn".....


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



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OfflineKulkulcan
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Re: keep PF spawn strong until G2G? [Re: Kenetic]
    #23985049 - 01/05/17 08:12 PM (7 years, 25 days ago)

If there's no sign of contams in either the dry layer or in the fully colonized spawn, why not just leave the jars sealed until you're ready for G2G?


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OfflineKenetic
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan]
    #23985054 - 01/05/17 08:14 PM (7 years, 25 days ago)

How do you know if the verm layer has contams or not?  Until you spawn at least.

Maybe it's possible to "g2g" pf tech but it's certainly not practical


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Invisibler.lutece
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Re: keep PF spawn strong until G2G? [Re: Kenetic]
    #23985208 - 01/05/17 09:16 PM (7 years, 25 days ago)

Quote:

kenetic said:
How do you know if the verm layer has contams or not?  Until you spawn at least.




:whathesaid:

The great thing about a dry verm layer is that the contams can't germinate in it, but once you give them some other materials to jump to, they can pick right up. Remember that the verm is your filter material, so you want to remember to not only knock off that verm, but also rinse the remaining verm off the cake. And you'll still probably see some contamination even after those precautions are taken. Which is part of the danger of spawning with cakes.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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OfflineKulkulcan
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Re: keep PF spawn strong until G2G? [Re: Kenetic]
    #23986302 - 01/06/17 11:15 AM (7 years, 24 days ago)

Quote:

kenetic said:
Maybe it's possible to "g2g" pf tech but it's certainly not practical



You can't just shred a PF cake and G2G it into large jars of loose grain (in a SAB or FH, of course)? Looking at "6T WBS Tek (Wild Bird Seed) Bulk Spawn Preparation Method", there doesn't appear to be an obstacle.


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Invisibleamidogen
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan]
    #23986354 - 01/06/17 11:40 AM (7 years, 24 days ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 01:07 PM)


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InvisibleAdden
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Re: keep PF spawn strong until G2G? [Re: amidogen]
    #23986361 - 01/06/17 11:44 AM (7 years, 24 days ago)

Quote:

amidogen said:
If you can prep grains, you can prep agar.




"If you can dodge a wrench, you can dodge a ball." :lol:

Almost every PF jar set is bacterial in one way or another you see it all the time here just do grains.


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OfflineKulkulcan
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Re: keep PF spawn strong until G2G? [Re: Adden]
    #23986809 - 01/06/17 03:17 PM (7 years, 24 days ago)

OK, so if you already have some fully colonized PF cakes, then maybe the best way to go from there would be to bulk substrate...?


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Invisibleamidogen
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan]
    #23986839 - 01/06/17 03:26 PM (7 years, 24 days ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 01:07 PM)


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OfflineKulkulcan
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Re: keep PF spawn strong until G2G? [Re: amidogen]
    #23987135 - 01/06/17 05:09 PM (7 years, 24 days ago)

How risky is it? The 3 or 4 videos I came across made it look easy enough. But maybe it IS too risky, I don't know, that's why I'm asking. But anyway, if you had several cakes and didn't care about getting very many fruits, you could try spawning a couple of the cakes to a bulk substrate, just to pursue the bobby, you know? But maybe if it's quite risky, then you wouldn't want to try it. So based on all that, what do you think?


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Invisibleamidogen
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan]
    #23987356 - 01/06/17 06:19 PM (7 years, 24 days ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 01:07 PM)


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Invisibler.lutece
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Re: keep PF spawn strong until G2G? [Re: amidogen]
    #23987438 - 01/06/17 06:47 PM (7 years, 24 days ago)

As someone currently messing around with cakes (only living in this dump for four more months, don't feel like unpacking all my stuff), they are SO much more work compared to a mono. God I miss just forgetting I was even growing mushrooms for like a week, and then checking to see they're doing just fine without me.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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OfflineKulkulcan
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Re: keep PF spawn strong until G2G? [Re: r.lutece]
    #23989002 - 01/07/17 10:03 AM (7 years, 23 days ago)

Thanks to everybody for their input.

One last question: Am I right that no one thinks that just CASING fully colonized PF spawn would be risky?


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InvisibleAdden
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan]
    #23989069 - 01/07/17 10:28 AM (7 years, 23 days ago)

You actually do case a PF jar when you roll it.. it helps keep a 1-2mm microclimate in little pockets in the network where pins will grow.. which is why it's important to wait a day or two after rolling before you begin misting. But anyway, I've seen people do trays and use them as spawn and it's always lackluster, and what's a gram or two more.. and time could be better spent on other things.. I mean, I still play with PF because it's designed to be so simple and easy and all-in-one.


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OfflineKenetic
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan]
    #23989101 - 01/07/17 10:40 AM (7 years, 23 days ago)

Might work.  Do whatever you want to do, but breaking up and casing a pf jar will just set you back 4 or 5 days for similar results.  Youre just hurting the network by breaking it up


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleAdden
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Re: keep PF spawn strong until G2G? [Re: Kenetic]
    #23989118 - 01/07/17 10:45 AM (7 years, 23 days ago)

Well, that and why put a tray in a SGFC for maybe a few grams when you could have six cakes using that space.


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OfflineLobi
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Re: keep PF spawn strong until G2G? [Re: Adden]
    #23989403 - 01/07/17 12:13 PM (7 years, 23 days ago)

here is half a pf cake to oats after 3 days. I have split it up to one cake to 4 qt jars of grain and it work out decently. I dump the dry verm layer in sab into an empty jar and dump the cake into a big ziploc bag and crush it up. then i pour it from the bag into the grain jar. you can also use Lazy Mofo Bag Tek and then crush that up and split it into 10 jars of grain and that should colonize in 4 or 5 days as well. i have had great success with this just to see if it works. I would recommend just doing regular g2g with agar masters tho..



--------------------
The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship.
The fellowship of atoms,
of star dust in its endless flight, of suns and worlds,  of gods and men.
The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit.

:mushroom2:My High Quality Lo-Fi Beats  -:mushroom2:MushroomCultivation Compendium  -:mushroom2:- Doing Bulk w/ No PC -:mushroom2:

:chillpill:more about my music :chillpill:


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OfflineKulkulcan
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Re: keep PF spawn strong until G2G? [Re: Lobi]
    #23990280 - 01/07/17 05:42 PM (7 years, 23 days ago)

Quote:

Lobi said:
here is half a pf cake to oats after 3 days. I have split it up to one cake to 4 qt jars of grain and it work out decently. I dump the dry verm layer in sab into an empty jar and dump the cake into a big ziploc bag and crush it up. then i pour it from the bag into the grain jar. you can also use Lazy Mofo Bag Tek and then crush that up and split it into 10 jars of grain and that should colonize in 4 or 5 days as well. i have had great success with this just to see if it works. I would recommend just doing regular g2g with agar masters tho..



Wow, I guess the speed of colonization is what makes this work. That, and the bag tek seems like a smart low-tech way to reduce the introduction of contams. If you had a few cakes, this seems like it would be worth trying with one of them. Thanks for the info.


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OfflineKulkulcan
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Re: keep PF spawn strong until G2G? [Re: Adden]
    #23990360 - 01/07/17 06:09 PM (7 years, 23 days ago)

Quote:

Adden said:
Almost every PF jar set is bacterial in one way or another you see it all the time here just do grains.



Just out of curiosity, why is bacterial contamination so much more common in PF than in loose grain?


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OfflineLobi
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan]
    #23990407 - 01/07/17 06:26 PM (7 years, 23 days ago)

Quote:

Kulkulcan said:
Quote:

Lobi said:
here is half a pf cake to oats after 3 days. I have split it up to one cake to 4 qt jars of grain and it work out decently. I dump the dry verm layer in sab into an empty jar and dump the cake into a big ziploc bag and crush it up. then i pour it from the bag into the grain jar. you can also use Lazy Mofo Bag Tek and then crush that up and split it into 10 jars of grain and that should colonize in 4 or 5 days as well. i have had great success with this just to see if it works. I would recommend just doing regular g2g with agar masters tho..



Wow, I guess the speed of colonization is what makes this work. That, and the bag tek seems like a smart low-tech way to reduce the introduction of contams. If you had a few cakes, this seems like it would be worth trying with one of them. Thanks for the info.



I made 5 bags to test out an old Tek I did using the bags as spawn, ended up with 10 so I used half of them to g2g 1:10 into oats. Spawned them all into 8 66qt tubs. PE and just regular cubes. I'm doing a log on it rn and will make a thread when it's pinning.


--------------------
The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship.
The fellowship of atoms,
of star dust in its endless flight, of suns and worlds,  of gods and men.
The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit.

:mushroom2:My High Quality Lo-Fi Beats  -:mushroom2:MushroomCultivation Compendium  -:mushroom2:- Doing Bulk w/ No PC -:mushroom2:

:chillpill:more about my music :chillpill:


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InvisiblebodhisattaMDiscordReddit
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Re: keep PF spawn strong until G2G? [Re: Kulkulcan] * 2
    #23990451 - 01/07/17 06:41 PM (7 years, 23 days ago)

Quote:

Kulkulcan said:
Quote:

Adden said:
Almost every PF jar set is bacterial in one way or another you see it all the time here just do grains.



Just out of curiosity, why is bacterial contamination so much more common in PF than in loose grain?



spore syringe inoculation, and not being pressure cooked.


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