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OfflineRealityCzech
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Indoor Cultivation of Puffballs
    #23983593 - 01/05/17 10:48 AM (7 years, 25 days ago)

Alright, so this is going to sound a bit odd.  I have a lightly wooded depression near my house that I gather puffballs from every year.  I was thinking that I might be able to biopsy and clone a puffball this year (I've got a very long time before they'll be popping up), but I've been unable to find anything at all regarding cultivation, temperature range, etc.

Has anybody tried this?  Is there no info because they simply don't grow well in a controlled environ?  If you have tried this, do you have notes regarding incubation temperatures, fruiting conditions, etc.?  I did find one old thread where someone was talking about having cultured mycelium from a wild puffball on agar, but nothing beyond the fact that he had done so.

If nobody knows, I might rewrite the code on my old Teensy based thermostat as a data logger and set it up where they grow to record humidity, temperature, etc. so I can get a good idea of the general growing and fruiting conditions in the wild to work from.  From there, I'll try to clone out one of the young puffballs I gather to eat this year rather than wait for the gleba to form and gather spore.

If anybody has any information on the domesticated cultivation of puffballs I'd really appreciate the assist.


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OfflinepoofterFroth
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Re: Indoor Cultivation of Puffballs [Re: RealityCzech]
    #23983688 - 01/05/17 11:37 AM (7 years, 25 days ago)

Very interested in this but on an outdoor level. I found and attempted cloning a giant puffball this past season and another smaller one. The smaller of the two took to agar very easily and was clean. Now it's chillin' in the fridge on a plate waiting for spring.

My plan is to run a large batch of spawn and liquid inoculates to spread around my fields and woods. The puffballs that I found and cloned were growing from the ground with a pretty substantial root, I imagine that wood-growing puffballs would take to indoor cultivation with less effort. Either wood based substrate blocks or logs like Woodears are grown.

And the damn things continued to grow after they were picked, the big one matured and started going to spore before rotting. The second one was put directly in the fridge which slowed or even stopped growth, and actually had a great shelf life.


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OfflineMrcloudy
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Re: Indoor Cultivation of Puffballs [Re: poofterFroth]
    #23983720 - 01/05/17 11:48 AM (7 years, 25 days ago)

I have cloned them but never bothered to try to grow them because the mycelium was just too slow.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflineRealityCzech
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Re: Indoor Cultivation of Puffballs [Re: poofterFroth]
    #23983726 - 01/05/17 11:50 AM (7 years, 25 days ago)

A few years back I picked my puffballs, set two on a shelf until they got that dark depression that tells you they're full of spores, and then burst them in a wooded area of my back yard.  I was hoping to have them growing close to home but since then, nothing at all.  Oh well, it was a crap shoot anyway.

From where the puffballs are growing wild, I'm pretty certain they're decomposing leaf litter and other woody stuff.  It's not too far off a walking path in the middle of a city with only rabbits, squirrels, and mice living around there.  The only other source of feces I can think of would be people's dogs off of their leashes. 

At any rate, I'm feeling confident that these are not coprophilic and I should be able to find an acceptable substrate.  It's the temperatures and humidity I'm concerned about.  If I get a good master culture started, I guess I can try running it with a whole range of options to see what works.  I was just hoping for a starting point.  It doesn't really matter yet, as I've got many months of planning time before I'll be able to forage anything anyway.


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OfflineRealityCzech
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Re: Indoor Cultivation of Puffballs [Re: Mrcloudy]
    #23983735 - 01/05/17 11:52 AM (7 years, 25 days ago)

Quote:

Mrcloudy said:
I have cloned them but never bothered to try to grow them because the mycelium was just too slow.




Any info on incubation temperature, etc.?  Anything is better than the vacuum I'll be working in now.


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OfflineMrcloudy
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Re: Indoor Cultivation of Puffballs [Re: RealityCzech]
    #23983767 - 01/05/17 12:05 PM (7 years, 25 days ago)

Temperature didn't seem to matter. It grew so slowly regardless. Sat at room temperature for months and never got any bigger than a half dollar before I decided to put it in the fridge to keep it from dying.

I have read that the addition of sugars to the agar might rectify the slowness. Perhaps wood tea agar.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflineM8M
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Re: Indoor Cultivation of Puffballs [Re: RealityCzech]
    #23985962 - 01/06/17 08:21 AM (7 years, 24 days ago)

I grew one outdoors just for fun to see if possible about 5 years ago.  See post #15196881

M8M


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