|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Rakin
Stranger


Registered: 11/17/16
Posts: 99
|
Re: Pasty Agar Tek [Re: TheDuder]
#23976059 - 01/02/17 12:43 PM (7 years, 1 month ago) |
|
|
Quick question. I've made a couple of batches already and done some successful transfers. But I have a few plates that my dyed green agar is turning yellow. They are fully colonized plates and look clean. Could it be from penetrating the agar with my instrument when doing the transfers? No contaminates on the surface at all
Edited by Rakin (01/02/17 12:45 PM)
|
AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 6 months
|
Re: Pasty Agar Tek [Re: Rakin]
#23976077 - 01/02/17 12:50 PM (7 years, 1 month ago) |
|
|
That's normal! The mycelium is eating up the agar and the food coloring changes color as it goes throughout it all. Scared me too when mine started yellowing as well.
|
Rakin
Stranger


Registered: 11/17/16
Posts: 99
|
Re: Pasty Agar Tek [Re: AK1000]
#23976328 - 01/02/17 02:14 PM (7 years, 1 month ago) |
|
|
Quote:
AK1000 said: That's normal! The mycelium is eating up the agar and the food coloring changes color as it goes throughout it all. Scared me too when mine started yellowing as well.
That's what I thought. The first one happened after I took the first transfers from it and I threw it away. Then a couple of the second transfers started doing it and I thought maybe it was from penetrating the agar. So I still have those plates.
|
danjaone
Entrepreninja


Registered: 03/19/10
Posts: 12
Loc: 42 miles into your brain
Last seen: 3 years, 4 months
|
|
so i am only able to find agar agar that has sugar in it around my area. is this un-usable or just not a good idea?
-------------------- Even chaos has a melody.... act accordningly
|
Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
Last seen: 3 years, 1 month
|
Re: Pasty Agar Tek [Re: danjaone]
#23976452 - 01/02/17 03:02 PM (7 years, 1 month ago) |
|
|
Quote:
danjaone said: so i am only able to find agar agar that has sugar in it around my area. is this un-usable or just not a good idea?
youd be better off ordering some of the pure stuff online
|
synthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 11 months
|
|
love this tek, thanks for taking the time to post! got a clone of a cyan goin now, and going to try a print soon...
one question, is there a benefit to me staying with mini rounds? 60mm petris would take up a lot less room in my small space, thanks for your input!
|
NDStepp84
Stellar nuclear waste

Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 28 days
|
|
From my understanding you can't do no pour in petris, plastic will melt in the PC and glass will boil over. You have to use a pour tek with petris
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
|
synthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 11 months
|
|
Quote:
NDStepp84 said: From my understanding you can't do no pour in petris, plastic will melt in the PC and glass will boil over. You have to use a pour tek with petris
ahhhh.... thank you for the clarification! no why the fuck would they not make pc able pp5 petri's? odd...
|
Sarah Tonin
Gardener Gone Astray


Registered: 04/01/14
Posts: 4,396
Loc: out rageous
Last seen: 1 month, 23 days
|
|
Glass petri's work... not sure how that myth began unless it was with some very short-sided petri dishes. 15mm sides and above have worked for me. They are more difficult to handle, expensive, etc but they do take up less room and have kept me from throwing out plastic petris by the zillions.
|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 4 hours, 10 minutes
|
|
I think it's because they usually autoclave and pour agar in the lab instead of trying to keep dishes sterile while handling them outside of their flowhood
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
|
Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 17 days, 7 hours
|
|
I just see no reason to no pour petris, it's not like pouring is difficult whatsoever.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
|
ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
|
Re: Pasty Agar Tek [Re: Tiamo] 1
#23979060 - 01/03/17 02:47 PM (7 years, 1 month ago) |
|
|
Obtaining petri dishes is a little more work than buying glad rounds.
Agar work can be intimidating at first and ordering petris along with all other supplies might seem to be too much of a commitment or investment for some who haven't even dipped their feet in yet.
No pour is a perfect stepping stone for people getting into agar. It's actuallt so damn convenient that some people haven't bothered switching for years
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
|
Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 17 days, 7 hours
|
|
You misunderstood. I think the no pour is a great tek for glad rounds. But I have no idea why anyone would get petri dishes and then somehow turn them into a no pour tek. It's just so easy to pour petris.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
|
ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
|
Re: Pasty Agar Tek [Re: Tiamo]
#23979105 - 01/03/17 03:12 PM (7 years, 1 month ago) |
|
|
Whoops you're right. I read your comment out of context for some reason
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: Tiamo]
#23979133 - 01/03/17 03:25 PM (7 years, 1 month ago) |
|
|
Quote:
Tiamo said: You misunderstood. I think the no pour is a great tek for glad rounds. But I have no idea why anyone would get petri dishes and then somehow turn them into a no pour tek. It's just so easy to pour petris.
Word. Pour your petris. That's what they were designed for.
If your really paranoid about contams pour them hot. That way nothing that gets in during the pour will survive.
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 4 months
|
Re: Pasty Agar Tek [Re: MudaFuka]
#23979726 - 01/03/17 07:21 PM (7 years, 1 month ago) |
|
|
Or you can pour them like a boss at 118° f
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
synthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 11 months
|
Re: Pasty Agar Tek [Re: MudaFuka]
#23979996 - 01/03/17 08:51 PM (7 years, 1 month ago) |
|
|
hmmm I will admit now I didnt really even think about pouring agar I did some rounds and played with some spores just to test my contamination abilities, plates look clean but I think Ill go with petri's and pouring if just for the sake of saving space in my little fridge, thanks for the input, and sorry to derail this awesome tread pasty plates are the shit for sure... in a good way
|
ThaDragonMaster
Tha DragonMaster



Registered: 04/11/03
Posts: 5
Loc: Missouri
Last seen: 6 years, 11 months
|
|
I was wondering if you have to wrap them glad wrap or PARAFILM?? or do you not have to wrap them??
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 15 days
|
|
You do not have to wrap them. The lid of the PP5 containers should fit good enough. After some PC cycles it might be better to use parafilm because of the potential deformation of the lid. If you have some parafilm laying around, you could use it but it's not neccessary.
|
ThaDragonMaster
Tha DragonMaster



Registered: 04/11/03
Posts: 5
Loc: Missouri
Last seen: 6 years, 11 months
|
|
cool thanks for the info all i need to know.
|
|