Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineblackout
 User Gallery

Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23995431 - 01/09/17 02:40 PM (7 years, 21 days ago)

Quote:

natedawgnow said:
They were pretty bad. Lots of liquid in the bottom and hard to break up.




were these jars old by any chance? I remember mycofile posting these pics of "overincubated grains" which he said lead to very potent shrooms

Quote:

mycofile said:
Quote:

Allowing your spawn to incubate for months after colonization is NOT a good thing, no matter who tells you different



Naw, you're right.  Incubating spawn for months is a bad thing.  It ends up pissing all over itself like this:



Yeah, Who would use such nasty looking spawn?  I mean, who cares if it can grow the most potent shrooms you've ever dreamed about?  The flushes are bound to be wimpy, right?  I mean, didn't stamets say that spawn MUST be refrigerated if it can't be used at peak vitality (within just a few days of colonization)?  And didn't he have some ominous warning for people who dared to do differently?  Well, I read his words too.  And I expected fruits from over-incubated spawn to be pidly, low yielding, mutated, and generally fucked up.  Well, then explain this, which came from that bag above:



Don't believe everything you read, try it for yourself.  Unless you still have some theoretical reason why over-incubation is bad?




EDIT: just see the previous posts now, they do not look particularly old.


Edited by blackout (01/09/17 02:42 PM)


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: blackout]
    #23995446 - 01/09/17 02:48 PM (7 years, 21 days ago)

Yes and no. The first jar, which is responsible for the bag above, was
not consolidated at all. It was showing signs of bacteria before it
ever finished colonizing, I spawned it as soon as it finished.

The other 5 that I spawned were consolidated about a week because I was
busy and didn't get around to spawning. They were all part of the same
G2G and all showed signs of bacteria before 100% completion.

The comparison jar IS over consolidated, as I just cased it a few days
ago. But still, not that long. And it showed signs of bacteria long before colonization
just like the others, just it's had a little while longer to run its
course since it's still in the jar.

Now this is also not the first time I've done this, but it is the
first time I can do an actual comparison with a clone culture. Based
on first flush yield alone and being able to just reuse items and free
up jars is a plus in my book.


--------------------


Edited by natedawgnow (01/09/17 02:49 PM)


Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 3 months
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23995535 - 01/09/17 03:29 PM (7 years, 21 days ago)

I think it's an excellent idea.  You have nothing to lose and the possibility to gain and the tek is not excessively complicated.  My top fruited jars suck and while I know some people have excellent yields, I'm not a tc or anything and generally don't always get full canopies.  I do spend a good amount of time prepping grains and jars though, and it really sucks to throw away bacterial jars after going through all that.

The bacteria we deal with, unlike mold, don't generally produce spores that spread throughout the air and your house and ruin future grows.  They're pretty much self-contained within whatever they inhabit.  They also die off fairly quickly in open air

Too bad I'm moving this week and yesterday I just threw away SEVEN old bacterial quarts I didn't want to spawn......


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Extras: Filter Print Post Top
InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: Kenetic]
    #23996270 - 01/09/17 07:09 PM (7 years, 21 days ago)

Dawgy bag :lol: clever name!
Definitely a good idea if you need to free up some jars in a pinch!


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


Extras: Filter Print Post Top
OfflinePezam
Edible Warrior
 User Gallery


Registered: 01/01/17
Posts: 97
Last seen: 6 years, 9 months
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23996570 - 01/09/17 08:49 PM (7 years, 21 days ago)

Quote:

natedawgnow said:
That's going by our definition of BE, which is one oz mush per quart of spawn.

327 grams going by the 10% dry weight rule should be around 32 grams,
which is over one oz. Plus the spawn was bacterial so it technically
should have produced less than optimal, but it produced better than
optimal in nothing but a spawn bag as a fruiting chamber.





Well played.


--------------------


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: Pezam]
    #24010709 - 01/14/17 08:03 PM (7 years, 16 days ago)

Updated the op and changed the title to make it a little more detailed and inclusive!


--------------------


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re:Testing Cultures? Bacterial spawn? Limited Space? Dawgy Bag it! [Re: natedawgnow] * 1
    #24025457 - 01/20/17 12:05 PM (7 years, 10 days ago)

Update:

Cased bag did better than the uncased for sure, which is good cause i cased
the tub of the same culture i'm running at the moment




The difference with these bags and the first bag I did is that these were unmodified. No
slits were cut and the bag was just left open for a good portion of the day with light mistings here and there.


Here are a couple uncased bags

This one didn't do too bad. The difference between the uncased bags with slits cut
and uncased bags with no slits is definitely noticeable! Uncased with slits cut did much better.
I can only imagine how the cased bag would have done if I cut slits in it's bag:strokebeard:

This bag and another bag are pretty far behind. I think they may have been more bacterial
than the others as they are showing more signs of stress

The other has about the same pinset but my phone died so I can't get pics of it quite yet:lol:

Here's the cased jar comparison. As you can see it is much slower than the bags and a lot
less dense of a pinset, plus the jar has been occupied now for a few weeks.


--------------------


Edited by natedawgnow (01/20/17 12:23 PM)


Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 3 months
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #24025489 - 01/20/17 12:21 PM (7 years, 10 days ago)

Excellent work!


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: Kenetic]
    #24025587 - 01/20/17 01:02 PM (7 years, 10 days ago)

Thanks man :awesome:


--------------------


Extras: Filter Print Post Top
OfflineThedenthead
 User Gallery


Registered: 04/19/16
Posts: 531
Last seen: 4 months, 27 days
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #24028052 - 01/21/17 11:52 AM (7 years, 9 days ago)

These seem like viable candidates for a dawgy bag?


--------------------
Nothing but my meandering thoughts.

An ounce of prevention is worth a pound of cure.


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: Thedenthead]
    #24028074 - 01/21/17 12:05 PM (7 years, 9 days ago)

Do it up, mange!!

Those seem a little on the bacterial side but not so bad that they won't recover in a bag.
Go for it and post up your results here if you want!

Update:

from this


To this


In 24 hours



So far, each bag has put out almost 1.5oz from 1 qt of spawn each :rockon:


--------------------


Edited by natedawgnow (01/21/17 03:39 PM)


Extras: Filter Print Post Top
OfflineThedenthead
 User Gallery


Registered: 04/19/16
Posts: 531
Last seen: 4 months, 27 days
Re: Bacterial spawn? Dawgy Bag it! [Re: Thedenthead]
    #24028244 - 01/21/17 01:38 PM (7 years, 9 days ago)

I'll keep ya posted. Bought a different brand of WBS for these jars and it had way too much cracked corn, turned to shit. Be nice to salvage em somewhat.


--------------------
Nothing but my meandering thoughts.

An ounce of prevention is worth a pound of cure.


Extras: Filter Print Post Top
OfflineThedillestpickle
cultured
Male


Registered: 02/02/16
Posts: 1,170
Loc: Canada Flag
Last seen: 3 years, 7 months
Re: Bacterial spawn? Dawgy Bag it! [Re: Thedenthead]
    #24029221 - 01/21/17 09:44 PM (7 years, 9 days ago)

Good idea, might have to try this out rather than dump my sub-par spawn. 

Do you just prep a bucket tek and use it as needed/ store until needed again?  If i prep a a full bucket I'll have leftovers that may sit for a few weeks.  Might go bad, but I guess it's worth a shot.


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: Thedillestpickle]
    #24029346 - 01/21/17 10:53 PM (7 years, 8 days ago)

No, it shouldn't go bad. That is what this tek is meant for. A low waste approach and an
easy way to test cultures and make use of less than optimal spawn. For the first bag,
I used nothing but left over bucket tek coir that I added a bit of water to in order to
get it back to capacity. All bags were made with bacterial spawn.

The other 5 bags were made by breaking off a small portion of a brick, enough for 4 hydrated
quarts before verm and gypsum were added, bacterial spawn (same clone) and that's it.


--------------------


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #24029627 - 01/22/17 03:42 AM (7 years, 8 days ago)

Quote:

natedawgnow said:
from this


To this


In 24 hours



So far, each bag has put out almost 1.5oz from 1 qt of spawn each :rockon:




:wizard: nice dude


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: blindingleaf]
    #24033026 - 01/23/17 11:21 AM (7 years, 7 days ago)

Ok guys all the bags finished up and the op is updated with pics and wet weights of each
bag.

Final wet weight harvest totaled 2354.4 grams for roughly 6 quarts of spawn :rockon:
I'll come and update with the dry weight once the last bag finishes drying


--------------------


Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow] * 1
    #24033270 - 01/23/17 01:35 PM (7 years, 7 days ago)

Fruiting in the bag is awesome, been doing it for a long time. Simple and low cost, though there is a waste factor involved.

One note about your Biologic Efficiency though.


Quote:

Biological efficiency of a yield is calculated by the fresh weight harvest over dry substrate weight used.

100 grams fresh yield
100 grams dry substrate        = 100% BE





It's not about the quart, it's about the weight of dry substrate.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,281
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: wildernessjunkie]
    #24033282 - 01/23/17 01:39 PM (7 years, 7 days ago)

Quote:

wildernessjunkie said:
Fruiting in the bag is awesome, been doing it for a long time. Simple and low cost, though there is a waste factor involved.

One note about your Biologic Efficiency though.


Quote:

Biological efficiency of a yield is calculated by the fresh weight harvest over dry substrate weight used.

100 grams fresh yield
100 grams dry substrate        = 100% BE





It's not about the quart, it's about the weight of dry substrate.




d'oh. i thought it was based off volume.. so its weight????.. gotcha. is it relative to each grain? how do you calculate each one? or do we not do that BS hence me thinking it was based of 1 quart per oz..????

fakk:confused:


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: wildernessjunkie]
    #24033302 - 01/23/17 01:44 PM (7 years, 7 days ago)

Quote:

wildernessjunkie said:
Fruiting in the bag is awesome, been doing it for a long time. Simple and low cost, though there is a waste factor involved.

One note about your Biologic Efficiency though.


Quote:

Biological efficiency of a yield is calculated by the fresh weight harvest over dry substrate weight used.

100 grams fresh yield
100 grams dry substrate        = 100% BE





It's not about the quart, it's about the weight of dry substrate.



If you had read my next comment after that one, I say that OUR definition is that 1qt=1oz for 100% BE, but in
actuality it is the equation you just put. In psych mush cult, 1qt to 1oz is accepted as 100% BE.

Also, the whole point of this tek is to limit waste. I reuse every spawn bag I have. The
first bag I pictured, the one at the top of the OP, is from a run I did 2 years ago...


--------------------


Edited by natedawgnow (01/23/17 02:39 PM)


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #24033309 - 01/23/17 01:47 PM (7 years, 7 days ago)

Quote:

natedawgnow said:
Ya sorry I guess I should have been more specific in that quote.

Our definition of 100% BE is one ounce per quart of spawn. anything over
1 ounce is over 100% BE.

To calculate actual biological efficiency, you divide the total yield
of fresh mushrooms by the dry weight of substrate used then multiply
by 100.




--------------------


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Limited Space Suggestions Mycelium 1,220 6 06/19/02 04:22 AM
by Banshee
* Honey as antibacterial in spawn? MushBee 238 7 11/01/21 02:15 PM
by CreonAntigone
* Spawn alternatives Doraiaky 431 8 11/02/21 05:50 AM
by SirPsycho
* Clean spawn checklist for the new grower.
( 1 2 3 4 ... 53 54 )
Stipe-n CapMDiscord 43,529 1,060 09/10/23 10:52 AM
by darkokiddo
* Spore Bacterial Infection?
( 1 2 all )
BladeLSD 12,139 34 05/21/03 03:30 PM
by Suntzu
* Bacterial infection but still growing Spunkee 2,458 5 04/11/03 10:00 AM
by afoaf
* Need help, better than rye grain spawn bag question….
( 1 2 3 all )
Juss2021 694 43 12/01/21 04:01 PM
by Smartattack
* Low Risk Poo Spawning Idea! Opinions Please!! Gthirteens 1,242 1 01/23/03 12:34 AM
by Roadkill

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,836 topic views. 24 members, 186 guests and 56 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.008 seconds on 13 queries.