|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
|
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
#23995431 - 01/09/17 02:40 PM (7 years, 21 days ago) |
|
|
Quote:
natedawgnow said: They were pretty bad. Lots of liquid in the bottom and hard to break up.
were these jars old by any chance? I remember mycofile posting these pics of "overincubated grains" which he said lead to very potent shrooms
Quote:
mycofile said:
Quote:
Allowing your spawn to incubate for months after colonization is NOT a good thing, no matter who tells you different
Naw, you're right. Incubating spawn for months is a bad thing. It ends up pissing all over itself like this:

Yeah, Who would use such nasty looking spawn? I mean, who cares if it can grow the most potent shrooms you've ever dreamed about? The flushes are bound to be wimpy, right? I mean, didn't stamets say that spawn MUST be refrigerated if it can't be used at peak vitality (within just a few days of colonization)? And didn't he have some ominous warning for people who dared to do differently? Well, I read his words too. And I expected fruits from over-incubated spawn to be pidly, low yielding, mutated, and generally fucked up. Well, then explain this, which came from that bag above:

Don't believe everything you read, try it for yourself. Unless you still have some theoretical reason why over-incubation is bad?
EDIT: just see the previous posts now, they do not look particularly old.
Edited by blackout (01/09/17 02:42 PM)
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Bacterial spawn? Dawgy Bag it! [Re: blackout]
#23995446 - 01/09/17 02:48 PM (7 years, 21 days ago) |
|
|
Yes and no. The first jar, which is responsible for the bag above, was not consolidated at all. It was showing signs of bacteria before it ever finished colonizing, I spawned it as soon as it finished.
The other 5 that I spawned were consolidated about a week because I was busy and didn't get around to spawning. They were all part of the same G2G and all showed signs of bacteria before 100% completion.
The comparison jar IS over consolidated, as I just cased it a few days ago. But still, not that long. And it showed signs of bacteria long before colonization just like the others, just it's had a little while longer to run its course since it's still in the jar.
Now this is also not the first time I've done this, but it is the first time I can do an actual comparison with a clone culture. Based on first flush yield alone and being able to just reuse items and free up jars is a plus in my book.
--------------------
Edited by natedawgnow (01/09/17 02:49 PM)
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
#23995535 - 01/09/17 03:29 PM (7 years, 21 days ago) |
|
|
I think it's an excellent idea. You have nothing to lose and the possibility to gain and the tek is not excessively complicated. My top fruited jars suck and while I know some people have excellent yields, I'm not a tc or anything and generally don't always get full canopies. I do spend a good amount of time prepping grains and jars though, and it really sucks to throw away bacterial jars after going through all that.
The bacteria we deal with, unlike mold, don't generally produce spores that spread throughout the air and your house and ruin future grows. They're pretty much self-contained within whatever they inhabit. They also die off fairly quickly in open air
Too bad I'm moving this week and yesterday I just threw away SEVEN old bacterial quarts I didn't want to spawn......
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
|
Re: Bacterial spawn? Dawgy Bag it! [Re: Kenetic]
#23996270 - 01/09/17 07:09 PM (7 years, 21 days ago) |
|
|
Dawgy bag clever name! Definitely a good idea if you need to free up some jars in a pinch!
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
|
Pezam
Edible Warrior



Registered: 01/01/17
Posts: 97
Last seen: 6 years, 9 months
|
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
#23996570 - 01/09/17 08:49 PM (7 years, 21 days ago) |
|
|
Quote:
natedawgnow said: That's going by our definition of BE, which is one oz mush per quart of spawn.
327 grams going by the 10% dry weight rule should be around 32 grams, which is over one oz. Plus the spawn was bacterial so it technically should have produced less than optimal, but it produced better than optimal in nothing but a spawn bag as a fruiting chamber.
Well played.
--------------------
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Bacterial spawn? Dawgy Bag it! [Re: Pezam]
#24010709 - 01/14/17 08:03 PM (7 years, 16 days ago) |
|
|
Updated the op and changed the title to make it a little more detailed and inclusive!
--------------------
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re:Testing Cultures? Bacterial spawn? Limited Space? Dawgy Bag it! [Re: natedawgnow] 1
#24025457 - 01/20/17 12:05 PM (7 years, 10 days ago) |
|
|
Update:
Cased bag did better than the uncased for sure, which is good cause i cased the tub of the same culture i'm running at the moment



The difference with these bags and the first bag I did is that these were unmodified. No slits were cut and the bag was just left open for a good portion of the day with light mistings here and there.
Here are a couple uncased bags
 This one didn't do too bad. The difference between the uncased bags with slits cut and uncased bags with no slits is definitely noticeable! Uncased with slits cut did much better. I can only imagine how the cased bag would have done if I cut slits in it's bag
This bag and another bag are pretty far behind. I think they may have been more bacterial than the others as they are showing more signs of stress
 The other has about the same pinset but my phone died so I can't get pics of it quite yet
Here's the cased jar comparison. As you can see it is much slower than the bags and a lot less dense of a pinset, plus the jar has been occupied now for a few weeks.
--------------------
Edited by natedawgnow (01/20/17 12:23 PM)
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
#24025489 - 01/20/17 12:21 PM (7 years, 10 days ago) |
|
|
Excellent work!
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Bacterial spawn? Dawgy Bag it! [Re: Kenetic]
#24025587 - 01/20/17 01:02 PM (7 years, 10 days ago) |
|
|
Thanks man
--------------------
|
Thedenthead



Registered: 04/19/16
Posts: 531
Last seen: 4 months, 27 days
|
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
#24028052 - 01/21/17 11:52 AM (7 years, 9 days ago) |
|
|
These seem like viable candidates for a dawgy bag?
-------------------- Nothing but my meandering thoughts.
An ounce of prevention is worth a pound of cure.
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Bacterial spawn? Dawgy Bag it! [Re: Thedenthead]
#24028074 - 01/21/17 12:05 PM (7 years, 9 days ago) |
|
|
Do it up, mange!!
Those seem a little on the bacterial side but not so bad that they won't recover in a bag. Go for it and post up your results here if you want!
Update:
from this

To this

In 24 hours

So far, each bag has put out almost 1.5oz from 1 qt of spawn each
--------------------
Edited by natedawgnow (01/21/17 03:39 PM)
|
Thedenthead



Registered: 04/19/16
Posts: 531
Last seen: 4 months, 27 days
|
Re: Bacterial spawn? Dawgy Bag it! [Re: Thedenthead]
#24028244 - 01/21/17 01:38 PM (7 years, 9 days ago) |
|
|
I'll keep ya posted. Bought a different brand of WBS for these jars and it had way too much cracked corn, turned to shit. Be nice to salvage em somewhat.
-------------------- Nothing but my meandering thoughts.
An ounce of prevention is worth a pound of cure.
|
Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
|
Re: Bacterial spawn? Dawgy Bag it! [Re: Thedenthead]
#24029221 - 01/21/17 09:44 PM (7 years, 9 days ago) |
|
|
Good idea, might have to try this out rather than dump my sub-par spawn.
Do you just prep a bucket tek and use it as needed/ store until needed again? If i prep a a full bucket I'll have leftovers that may sit for a few weeks. Might go bad, but I guess it's worth a shot.
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
|
No, it shouldn't go bad. That is what this tek is meant for. A low waste approach and an easy way to test cultures and make use of less than optimal spawn. For the first bag, I used nothing but left over bucket tek coir that I added a bit of water to in order to get it back to capacity. All bags were made with bacterial spawn.
The other 5 bags were made by breaking off a small portion of a brick, enough for 4 hydrated quarts before verm and gypsum were added, bacterial spawn (same clone) and that's it.
--------------------
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
#24029627 - 01/22/17 03:42 AM (7 years, 8 days ago) |
|
|
Quote:
natedawgnow said: from this

To this

In 24 hours

So far, each bag has put out almost 1.5oz from 1 qt of spawn each 
nice dude
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Bacterial spawn? Dawgy Bag it! [Re: blindingleaf]
#24033026 - 01/23/17 11:21 AM (7 years, 7 days ago) |
|
|
Ok guys all the bags finished up and the op is updated with pics and wet weights of each bag.
Final wet weight harvest totaled 2354.4 grams for roughly 6 quarts of spawn I'll come and update with the dry weight once the last bag finishes drying
--------------------
|
wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
|
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow] 1
#24033270 - 01/23/17 01:35 PM (7 years, 7 days ago) |
|
|
Fruiting in the bag is awesome, been doing it for a long time. Simple and low cost, though there is a waste factor involved.
One note about your Biologic Efficiency though.
Quote:
Biological efficiency of a yield is calculated by the fresh weight harvest over dry substrate weight used.
100 grams fresh yield 100 grams dry substrate = 100% BE
It's not about the quart, it's about the weight of dry substrate.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
|
Quote:
wildernessjunkie said: Fruiting in the bag is awesome, been doing it for a long time. Simple and low cost, though there is a waste factor involved.
One note about your Biologic Efficiency though.
Quote:
Biological efficiency of a yield is calculated by the fresh weight harvest over dry substrate weight used.
100 grams fresh yield 100 grams dry substrate = 100% BE
It's not about the quart, it's about the weight of dry substrate.
d'oh. i thought it was based off volume.. so its weight????.. gotcha. is it relative to each grain? how do you calculate each one? or do we not do that BS hence me thinking it was based of 1 quart per oz..????
fakk
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
|
Quote:
wildernessjunkie said: Fruiting in the bag is awesome, been doing it for a long time. Simple and low cost, though there is a waste factor involved.
One note about your Biologic Efficiency though.
Quote:
Biological efficiency of a yield is calculated by the fresh weight harvest over dry substrate weight used.
100 grams fresh yield 100 grams dry substrate = 100% BE
It's not about the quart, it's about the weight of dry substrate.
If you had read my next comment after that one, I say that OUR definition is that 1qt=1oz for 100% BE, but in actuality it is the equation you just put. In psych mush cult, 1qt to 1oz is accepted as 100% BE.
Also, the whole point of this tek is to limit waste. I reuse every spawn bag I have. The first bag I pictured, the one at the top of the OP, is from a run I did 2 years ago...
--------------------
Edited by natedawgnow (01/23/17 02:39 PM)
|
natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
|
Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
#24033309 - 01/23/17 01:47 PM (7 years, 7 days ago) |
|
|
Quote:
natedawgnow said: Ya sorry I guess I should have been more specific in that quote.
Our definition of 100% BE is one ounce per quart of spawn. anything over 1 ounce is over 100% BE.
To calculate actual biological efficiency, you divide the total yield of fresh mushrooms by the dry weight of substrate used then multiply by 100.
--------------------
|
|