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Invisiblenatedawgnow
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Testing cultures? Bacterial spawn? Limited Space? Dawgy Bag it! * 4
    #23981421 - 01/04/17 01:10 PM (7 years, 26 days ago)


Dawgy Bags:lol:

A simple, low waste approach to testing cultures and dealing
with less than optimal spawn

What's up every one!

So I altered this post a little bit to be more encompassing as I
realized these are also a great way to test out clone/isolate cultures!

Let's say you have 15 cultures to test out, do you really want to make
15 mini tubs? What a pain in the ass, right? Well these bags are great
for testing cultures as they are their own fruiting chambers and
require very little maintenance.

I also see a lot of bacterial spawn posts here on the boards. No one wants to expand it, some
people say to just throw it out even. Most will say it's ok to spawn it
but spawn it alone and separately from everything else.

Ok, so what are your options? Spawn it to a mini tub? That takes up a lot
of space for just 1 or 2 jars.

You can top fruit the jar; but now you can't use it for more spawn for
another couple weeks. I don't have a million jars so I like to flip mine
as fast as possible.

Another thing about me is I like to reuse and reduce when I can.

Enter Dawgy Bags.

Spawn bags can be used more than once. Cook some grain and then after
you spawn the grain, you can re-use the bag to spawn your bacterial grain jars for a super
simple colonization/fruiting chamber.

Or just dedicate a couple bags specifically for this purpose. They make
great fruiting chambers. After full colonization, just cut a couple
slits in the bag or even just open the bag and keep it open for a good
majority of the day, either way works. I've even left the bags closed
with no air exchange other than the filter patch till harvest.

Some pics: Bacterial spawn

Had a bacterial outbreak after a G2G or the master had some hidden
bacteria, either way I had 6 bacterial jars and didn't want to just
chuck em.

Here is the first jar spawned to 1qt of CVG. You can use excess cvg or
just make enough for a couple quarts, or however many you need. I just
measure out a quart give or take of sub for each bag. A little more
never hurt.


Plenty of pins in this bag. Colonized in 4 days and it's been in fruiting
since the 30th. Not too bad for a bacterial quart.

The other bags I just spawned the other night after some consolidation
as I've been pretty busy the past week :facepalm:



Old grow where I tried to make a bag/mono hybrid :lol:
It worked just not the best


Top fruiting vs. Bags/Cased vs. Uncased:
So I decided to do a comparison since these jars are all from the
same clone, and I decided to case and top fruit a jar along side the
dawgy bags.

Here is a pic of the jar along with a couple pics of dawgy bags, one
cased and one uncased, to test and see which way the culture prefers.

cased jar:


Cased bag:


Uncased bag:


As you can see, the jar, although cased a few days after the bags were
spawned, is far behind the bags. The cased bag has a much more uniform
pinset and much more knots than the uncased bag as well.

Only thing left is to see if the cased bag yields more than the 1st
bag I harvested which was uncased and put out 327 fresh grams 1st flush! Hard to beat but maybe!

Once you are ready to harvest just twist and pull them out or you can
remove the whole block from the bag. To dunk, just pour a little water
in the bag and twist it to keep the block from floating. You may not
wish to dunk and just pitch after the first flush depending on how
bad your jars were.

These bags take up very little space. You can just put them on a shelf,
on the table, hang them in the closet, or put them on the floor in the
corner.

So if you're like me and you have reservations about throwing out jars
that can still produce, or are low on space for clone testing, give this a try! It works with oven bags also
if you don't use spawn bags and don't feel like buying them anytime soon.

Grow Update

Bag 1



Bag 2 (cased comparison bag)



Bag 3

Forgot to get the canopy and final wet weight shot of this one but it was over 400g fresh

Bag 4



Bag 5



Bag 6



Results:

Bag 1 did the worst, which was surprising seeing as it got the most FAE since I cut slits
into that bag. I am kind of baffled by it to be honest, This culture must like less FAE:shrug:

The cased bag did the best at 435g, but it really wasn't that much better than the others.

I'll come back with total harvest dry weight later today when the last bag finishes drying!

The jar really shit the bed and has a crappy pinset, I'll post photos of that later as well
:cheers:


Edited by natedawgnow (01/23/17 11:19 AM)


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Invisiblenatedawgnow
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23993443 - 01/08/17 07:14 PM (7 years, 22 days ago)

Bag 1: PE6



I'll probably harvest this flush tomorrow. The veils that have broken
already are sporeless so I don't think there will be a sporesplosion.

I'll update with total harvest weights to show that bacterial spawn
can still produce well enough if you don't want to throw it out :cheers:


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Invisiblenatedawgnow
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23994775 - 01/09/17 10:21 AM (7 years, 21 days ago)

Even though I'm the only one following this thread, I'm still gonna
update it for any one who is interested in a no waste approach to cult.

So here are some pre/post harvest and wet weight pics for you guys

canopy shot



And wet weight


So that's 327 wet grams for 1 bacterial qt of spawn, left over bucket
tek cvg, and a used spawn bag. That is over 100% BE with no other
fruiting chamber but the bag itself and some, what amounts to, leftover
garbage:rockon:

I still have 5 more bags to go. Those are actual spawn bags that I
dedicated to this purpose, but they will be reused over and over after
this grow. They can be washed out or you can even pc them to clean them.
Just roll them up and stick them in a jar during your normal grain run


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OfflinePezam
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23994860 - 01/09/17 11:02 AM (7 years, 21 days ago)

I agree with your mindest, though I grow for bulk so it's worth it to weed out ANY contamination.  Anything with bacteria goes in the outdoor patch.
Cheers


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Offlinefatmatic
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23994861 - 01/09/17 11:03 AM (7 years, 21 days ago)

Could you just use ziplock bags as well if you have them laying around? I think I have a couple bacterial quart jars that I just dont want to throw out.


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InvisiblemushboyMDiscord
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Re: Bacterial spawn? Dawgy Bag it! [Re: Pezam]
    #23994869 - 01/09/17 11:05 AM (7 years, 21 days ago)

are those home made 'choke bags' i might be reading the OP wrong.
i used them before(14years ago) and had similar results.


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Invisiblenatedawgnow
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Re: Bacterial spawn? Dawgy Bag it! [Re: mushboy]
    #23994899 - 01/09/17 11:14 AM (7 years, 21 days ago)

No they are spawn bags. You can get them from any sponsor or ebay. I
am reusing them for the purpose of spawning bacterial spawn. I find I
get more out of the quart first flush if I spawn it this way vs. top
fruiting the jar and it frees up the jar for more projects.

I actually have a cased jar of the same clone going right now just to
compare first flush weights.

You can use any kind of bag for this, from big ziplocks to oven bags
and grocery bags. I use spawn bags for bulk and I see no reason to
throw them away if they can be repurposed. Our hobby has a lot of
waste as it is.

Pezam, I grow for bulk too, but not everyone has the ability to just put
their stuff outside. A lot of people live in rented homes/apartments and
do not feel comfortable with a contam pile, but also don't want to just
throw their spawn out if it can still produce, ya know?


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OfflineKenetic
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23995031 - 01/09/17 12:03 PM (7 years, 21 days ago)

:popcorn:

Will have more to say after work but hell yeah


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisiblemushboyMDiscord
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Re: Bacterial spawn? Dawgy Bag it! [Re: Kenetic]
    #23995033 - 01/09/17 12:04 PM (7 years, 21 days ago)

Quote:

natedawgnow said:
I find I get more out of the quart first flush if I spawn it this way vs. top
fruiting the jar and it frees up the jar for more projects.




cant argue with that:cheers:


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Invisiblefreespeech
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Re: Bacterial spawn? Dawgy Bag it! [Re: mushboy]
    #23995101 - 01/09/17 12:28 PM (7 years, 21 days ago)

Cool easy and low-effort tek :thumbup::thumbup:


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Invisiblenatedawgnow
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Re: Bacterial spawn? Dawgy Bag it! [Re: freespeech]
    #23995179 - 01/09/17 01:02 PM (7 years, 21 days ago)

Thanks guys!


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InvisiblemushboyMDiscord
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23995203 - 01/09/17 01:11 PM (7 years, 21 days ago)

how do you get the BE %%??:confused:


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Invisiblenatedawgnow
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Re: Bacterial spawn? Dawgy Bag it! [Re: mushboy]
    #23995210 - 01/09/17 01:15 PM (7 years, 21 days ago)

That's going by our definition of BE, which is one oz mush per quart of spawn.

327 grams going by the 10% dry weight rule should be around 32 grams,
which is over one oz. Plus the spawn was bacterial so it technically
should have produced less than optimal, but it produced better than
optimal in nothing but a spawn bag as a fruiting chamber.


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InvisiblemushboyMDiscord
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23995218 - 01/09/17 01:18 PM (7 years, 21 days ago)

Quote:

natedawgnow said:
That's going by our definition of BE, which is one oz mush per quart of spawn.





gotcha :takingnotes: thanks.


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Invisiblenatedawgnow
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Re: Bacterial spawn? Dawgy Bag it! [Re: mushboy]
    #23995229 - 01/09/17 01:21 PM (7 years, 21 days ago)

Ya sorry I guess I should have been more specific in that quote.

Our definition of 100% BE is one ounce per quart of spawn. anything over
1 ounce is over 100% BE.

To calculate actual biological efficiency, you divide the total yield
of fresh mushrooms by the dry weight of substrate used then multiply
by 100.


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Offlineblackout
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23995241 - 01/09/17 01:26 PM (7 years, 21 days ago)

Have you any pics of the bacterial jars before you spawned them? My understanding of the theory of casing jars/top fruiting jars is that you do not disturb the mycelium colony below as it is unlikely to recover if it was spawned or shaken -shaking jars being a common test to see if a jar is fucked.

I was expecting to see some technique for removing the grains with minimal breaking up, and just casing them, rather than mixing right up.

If its a very mild contamination you might get away with it of course, I just want others thinking of doing it to see how the jars looked.

One test I do rather than shaking the entire jar is to just tap it enough to disturb the top layer of grains, and see if it recovers. If there is no recovery I can scoop out those top grains and case

I like the idea of freeing up jars. I have many plastic containers which tend to be tapered, so I could "birth" them as a solid mass and put them into a similar size container, like a flower pot or some other plastic container which might not be heat resistant.


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Invisiblenatedawgnow
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Re: Bacterial spawn? Dawgy Bag it! [Re: blackout]
    #23995392 - 01/09/17 02:19 PM (7 years, 21 days ago)

They were pretty bad. Lots of liquid in the bottom and hard to break up.

I even used a spoon for 2 of them. I'll see if I can get a pic
of the liquid in the jar on the top fruited jar. The contamination is
definitely what most would consider past the point of mild.

I definitely should have included the pics of the jars originally but
I had the idea for the post after spawning them :lol:


--------------------


Edited by natedawgnow (01/09/17 02:20 PM)


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InvisiblemushboyMDiscord
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23995398 - 01/09/17 02:22 PM (7 years, 21 days ago)

that bacterial and it grew on the cvg? usually just with 'grains up on glass look' it goes green on me if i try to spawn. i top fruited a few and got something but nothing like your dawgy bag.


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Invisiblenatedawgnow
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23995409 - 01/09/17 02:29 PM (7 years, 21 days ago)

So this is the top fruited comparison jar from the same batch

You can see all the wet grains and the thick mycelium


In this pic you can clearly see the standing slimy water line in the
jar


I should mention that I did use a spoon to break up a couple of the
jars, thanks for the reminder blackout :cheers:

The rest though were shaken and they still recovered just fine :shrug:


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InvisiblemushboyMDiscord
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Re: Bacterial spawn? Dawgy Bag it! [Re: natedawgnow]
    #23995422 - 01/09/17 02:36 PM (7 years, 21 days ago)

i accidentally bought gigantic spawn bags and i have 3 quarts left of old bacterial leftovers
from the last MS grows..

i will be doing this in a few days with one of those jars. :thumbup:


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