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Tylershatto5
Psily Sybe


Registered: 12/29/16
Posts: 58
Loc: Indiana
Last seen: 6 years, 11 months
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Re: Agar In Syringe growth [Re: Grey]
#23981691 - 01/04/17 03:09 PM (7 years, 26 days ago) |
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i dont want to push the agar out it will be behind the water with the mycel that grew on it now in the water when i draw up sterile water it would then be broken up into the water bc of the pressure of the swirling water then press out until agar is all thats left then discard the old agar out the other end of the syringe the agar is thick so i dont plan to make any of it go through the needle
-------------------- I dont ask for opinions i ask for facts!
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KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
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Quote:
Tylershatto5 said: i dont want to push the agar out it will be behind the water with the mycel that grew on it now in the water when i draw up sterile water it would then be broken up into the water bc of the pressure of the swirling water then press out until agar is all thats left then discard the old agar out the other end of the syringe the agar is thick so i dont plan to make any of it go through the needle
How will you know if its got contamination inside the syringe? Isn't the whole point of agar so you can see it grow out and make sure it's contam free? Seems like it would be pretty hard to tell that inside a syringe, no? If you're sucking up spores onto the agar, you'll still have to wait a week or two for them to grow so why not just start with them on a small grain jar instead?
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Agar In Syringe growth [Re: KauaiOrca]
#23981873 - 01/04/17 04:33 PM (7 years, 26 days ago) |
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Colonize a pp5 of agar, PC a 10cc syringe, flame needle/eject, squirt into pp5 and stab/pull/stab/pull. Clean agar in a water suspension.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Quote:
Tylershatto5 said: i dont want to push the agar out it will be behind the water with the mycel that grew on it now in the water when i draw up sterile water it would then be broken up into the water bc of the pressure of the swirling water then press out until agar is all thats left then discard the old agar out the other end of the syringe the agar is thick so i dont plan to make any of it go through the needle
You still add an extra vector making the transfer into the syringe. How were you going to inoculate it?
If you manage to work the plunger without agitating the agar, once it's loose is gonna sink. I'm not saying it can't be done, but why? Your not really improving on anything with an extra vector. Can you list the steps for how you plan to prepare the syringe and inoculate it?
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Tylershatto5
Psily Sybe


Registered: 12/29/16
Posts: 58
Loc: Indiana
Last seen: 6 years, 11 months
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Re: Agar In Syringe growth [Re: Grey]
#23982049 - 01/04/17 05:27 PM (7 years, 26 days ago) |
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i just got a idea and wanted to try it and i have a piece to hook to syringes together for a safe transfer its a rig i made but i dont think im on to anything amazing im just doing it for the hell of it... trial n error i will learn something from it even if i just learn that i dont have the experience to do it shit i learned im just passing time while my jar inoculate
-------------------- I dont ask for opinions i ask for facts!
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Well if your just wanting a challenge or to practice sterile technique that's okay. I encourage you to try and let us know what you do. Maybe upload some pics also. I know everyone isn't visualizing this idea the same way.
Let us know how your colonizing stuff goes too.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Quote:
Tylershatto5 said: i just got a idea and wanted to try it and i have a piece to hook to syringes together for a safe transfer its a rig i made but i dont think im on to anything amazing im just doing it for the hell of it... trial n error i will learn something from it even if i just learn that i dont have the experience to do it shit i learned im just passing time while my jar inoculate
Yeah man play around. Not everyone is doing the same thing with the same exact tool on every tek with every perfect grade material PC'd or run like everyone else..
I really respect that you're taking time to do mad scientistry. I love playing around with different materials and ways of doing things that are easier on me.
Whatever works, as they say.
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KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
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Re: Agar In Syringe growth [Re: Adden]
#23982191 - 01/04/17 06:31 PM (7 years, 26 days ago) |
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Quote:
Adden said: Yeah man play around. Not everyone is doing the same thing with the same exact tool on every tek with every perfect grade material PC'd or run like everyone else..
I really respect that you're taking time to do mad scientistry. I love playing around with different materials and ways of doing things that are easier on me.
Whatever works, as they say.

So true ... some of the steps I take I'm almost positive no one else takes but I discovered them by experimenting and that's half the fun. I've thought before a good way to make LC would be right in syringe, but never tried it.
Try ... learn ... try again ... learn more ... in the end, you find out that cubes really do like to grow and if you give them half a chance, they'll fight off contamination and find a way to pin and fruit ...
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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