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OfflineTylershatto5
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Agar In Syringe growth
    #23979202 - 01/03/17 03:50 PM (7 years, 27 days ago)

01/03/17

okay so ive started an Agar syringe Well call it A.S. TEK

so i decided to make a PDA (potato dextrose Agar) and drawn up about 6ml expecting to lose a bit during PC (pressure Cooker) but i did this with 2 syringes one ill be adding a small live culture and one small amount of spores in the other

i seen a tread awhile back about doing agar upside down in baby food jar swirling the agar before it solidifies to get a small mount on the sides as a way to prevent contamination and not only did he prevent but he claimed to have got faster growth???

so i will pull my plunger back allowing agar to settle on the opposite side as the needle so mycelium will be easier to get out without contamination

i have very little experience with agar and i maybe doing something thats been done or is impossible but if not an it works sweet!!!

any info that may help or reasons why it wont work please let me kno ill post pictures as soon as agar cools


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InvisibleAdden
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Re: Agar In Syringe growth [Re: Tylershatto5]
    #23979206 - 01/03/17 03:52 PM (7 years, 27 days ago)

I don't think I'm understanding what you're trying to accomplish here.


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OfflineTylershatto5
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Re: Agar In Syringe growth [Re: Adden]
    #23979213 - 01/03/17 03:56 PM (7 years, 27 days ago)

to grow a live culture on agar but not in a petri dish or in a small jar im going to attempt to grow it inside the syringe so that i can just pull up some D H2O and squirt the mycelium that was living in the syringe to grain


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OfflineTiamo
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Re: Agar In Syringe growth [Re: Tylershatto5]
    #23979219 - 01/03/17 03:57 PM (7 years, 27 days ago)

What's the point??? :confused:


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OfflineFerather
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Re: Agar In Syringe growth [Re: Adden]
    #23979220 - 01/03/17 03:58 PM (7 years, 27 days ago)

If the needle is big enough, you will be pushing jelly like an icing bag.
It won't work the way you want it to, pushing contams out.

Your not removing contams, or isolating.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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InvisibleAdden
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Re: Agar In Syringe growth [Re: Tylershatto5]
    #23979227 - 01/03/17 04:01 PM (7 years, 27 days ago)

I think you're spinning your wheels trying to do something that will just make everything harder.

Are you not understanding LC or LI? I think that's what you're aiming for here, right? A syringe of active myc that you can bang into jars or bags?


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OfflineTylershatto5
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Re: Agar In Syringe growth [Re: Adden]
    #23979231 - 01/03/17 04:03 PM (7 years, 27 days ago)

that is it theres no contams i plan to just push some water with mycel in it out


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InvisibleAdden
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Re: Agar In Syringe growth [Re: Tylershatto5]
    #23979247 - 01/03/17 04:09 PM (7 years, 27 days ago)



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OfflineTylershatto5
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Re: Agar In Syringe growth [Re: Adden]
    #23979251 - 01/03/17 04:10 PM (7 years, 27 days ago)

i mad the agar quite thick and i have a glove box and i planed to do it all in there and incubate it in there with hopes of no contam im just bored and figured no one tried it before so i just wanted to see if i could make something work i have 25 pf jars and 4 mono tubs going and i got tired of watching jars so i needed to find something to do it cant hurt


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InvisibleAdden
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Re: Agar In Syringe growth [Re: Tylershatto5]
    #23979255 - 01/03/17 04:12 PM (7 years, 27 days ago)

Swap your glove box for an SAB.

You can just let jars sit in a cabinet or on a shelf they don't need to be incubated. Light during the entire process is beneficial.

What do your cakes and tubs look like?


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OfflineTylershatto5
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Re: Agar In Syringe growth [Re: Adden]
    #23979264 - 01/03/17 04:16 PM (7 years, 27 days ago)

i do use an incubation and i use CFL and a low wattage red n blue spec grow light i used for my plants whick they seem to grow more at night leaving only the red n blue on compared to no light at night i also use a heating pad check out my gro log to see my jars and progression

https://www.shroomery.org/forums/showflat.php/Number/23973317


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InvisibleAdden
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Re: Agar In Syringe growth [Re: Tylershatto5]
    #23979271 - 01/03/17 04:18 PM (7 years, 27 days ago)

Everything could be made simpler if you did a little reading.


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InvisiblemushboyMDiscord
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Re: Agar In Syringe growth [Re: Tylershatto5]
    #23979277 - 01/03/17 04:21 PM (7 years, 27 days ago)

Quote:

Tylershatto5 said:
i do use an incubation and i use CFL and a low wattage red n blue spec grow light i used for my plants whick they seem to grow more at night leaving only the red n blue on compared to no light at night i also use a heating pad check out my gro log to see my jars and progression

https://www.shroomery.org/forums/showflat.php/Number/23973317




thats a whole lotta wrong.:facepalm3:


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InvisibleKauaiOrca
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Re: Agar In Syringe growth [Re: Adden]
    #23979285 - 01/03/17 04:24 PM (7 years, 27 days ago)

Why not just grow a nice agar puck ... sterilize a pint jar with 200ml''s of water in it ... drop the colonized agar puck into the sterile pint and then just shake the shit out of it?  You can make 15 or more 10ml syringes from that.  Putting agar inside a syringe just doesn't sound like a smart thing to do at all.  What problem does that solve?


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"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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InvisibleAdden
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Re: Agar In Syringe growth [Re: mushboy]
    #23979293 - 01/03/17 04:26 PM (7 years, 27 days ago)

Quote:

mushboy said:
Quote:

Tylershatto5 said:
i do use an incubation and i use CFL and a low wattage red n blue spec grow light i used for my plants whick they seem to grow more at night leaving only the red n blue on compared to no light at night i also use a heating pad check out my gro log to see my jars and progression

https://www.shroomery.org/forums/showflat.php/Number/23973317




thats a whole lotta wrong.:facepalm3:




Indeed. It's a lot of outdated information. Things are a lot easier now.

And please understand OP. People here are trying to help.. so please don't lose your mind over stuff like a lot of people who are new (or returning) do.


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OfflineTylershatto5
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Re: Agar In Syringe growth [Re: Adden]
    #23979331 - 01/03/17 04:42 PM (7 years, 27 days ago)

well idk how u can have the nerve to say im all wrong but i have an experienced growing helping me and yes i maybe out dated bc my guys old school but out of 25 jars only one has a possible contam and ive got alot of info from this site so unless im going to be told whats wrong id rather not hear people just try to talk shit


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OfflineTiamo
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Re: Agar In Syringe growth [Re: Tylershatto5]
    #23979334 - 01/03/17 04:43 PM (7 years, 27 days ago)

I'm just wondering what the point is, you're not explaining yourself very well. It sounds to me you could accomplish the same with a LC.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisiblemushboyMDiscord
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Re: Agar In Syringe growth [Re: mushboy]
    #23979346 - 01/03/17 04:49 PM (7 years, 27 days ago)

Quote:

mushboy said:
Quote:

Tylershatto5 said:
i do use an incubation and i use CFL and a low wattage red n blue spec grow light i used for my plants whick they seem to grow more at night leaving only the red n blue on compared to no light at night i also use a heating pad check out my gro log to see my jars and progression

https://www.shroomery.org/forums/showflat.php/Number/23973317




thats a whole lotta wrong.:facepalm3:





unless your living space is below 65F you do not need an incubator. its a bacterial farm.

low wattage red/blue or whatver have no place in mushroom growing. nor does a 1100k in your thread.

never compare or translate knowledge of plants with mushrooms.

never ever ever use a heating pad. just leave jars on the shelf or something.

thats just that one sentence dude.
i didnt want to go all out on you but if you are going to reply to people helping you with 'this is my way' stuff you are going to get it handed to
you on a verity of levels. buck up.

and tell your 'old friend' to get with the program.

i wouldnt want to learn to drive from someone whos only driven a model t


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InvisibleAdden
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Re: Agar In Syringe growth [Re: Tylershatto5]
    #23979401 - 01/03/17 05:10 PM (7 years, 27 days ago)

Everything mush boy said.. and swap the glove box for an SAB..

Clone on no pour agar it's easier than making jello, and allows short PC times so you can run those materials early in the day and run other stuff at night.

If you hone your techniques then you can get a lot more mushrooms out of your spawn.. don't worry man this place is like a classroom everyone is willing to help just don't lash out.. I came back to growing after 15 years doing the same stuff you did, only to find it was all so wrong and I spent time reading stuff I didn't have to (and bought tons of stuff I didn't need to).


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Re: Agar In Syringe growth [Re: Adden]
    #23979866 - 01/03/17 08:09 PM (7 years, 27 days ago)

I've considered what your talking about, and it seems simpler than it is.  No matter how you do it, pushing even a small clump of agar through the syringe isn't easy.  You could make it firm,  but you'll still have leftover inoculate. If you do it "just right" you can get a good bit out, but not all. Then you've got a gummed up syringe.  Any thinner, and your just making an lc in a syringe,  nothing new. 

Bottom line,  every plan of attack added more vectors than I really cared to chance.  Pasty's new lc tek, the way Aero makes LI, and Munch's blenderless LI tek have a better chance at working. 

If you are thinking of a different way,  I would like to discuss it.


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