|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Any Benefit to adding Coffee to CC and Honey to DFA
#23976442 - 01/02/17 03:00 PM (7 years, 28 days ago) |
|
|
This question is two fold, because I didn't want to create more then one thread.
1. I am making dog food agar. I would like to add honey to the mix to see if there is a benefit. Is there any benefit to this or would this overly enrich the agar. Agar is expensive and I don't feel like throwing it out the window if anyone knows the answer. BTW all the recipes here are in grams. Am I the only person on this cite that doesn't have a scale. Teaspoon/tablespoons conversions would be a great help. 
Next
2. I am doing cracked corn for jars, because Rye berries are 10 times as expensive and cracked corn is $8.00 per 50lb bag. Is there any benefit to adding coffee water to the 30 minute heat/soak? Rye Berries soak for over half a day and I can see a benefit there, but in a 30 minute cooking process is the corn actually going to retain any of the coffee, and would myc benefit to adding nitrogen to cracked corn, as opposed to rye berries.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein] 1
#23976451 - 01/02/17 03:02 PM (7 years, 28 days ago) |
|
|
30min of heat to cracked corn will give you one nasty mess.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23976601 - 01/02/17 04:01 PM (7 years, 28 days ago) |
|
|
That's what the tek says in the cultivation section of this site. Can you elaborate and or answer my other questions.
Thanks for chiming in through
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23976624 - 01/02/17 04:12 PM (7 years, 28 days ago) |
|
|
A scale is a must IMO.
The cultivation section of this website is full of old garbage. Good research is done on the forums where there's time tested proven methods
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23976629 - 01/02/17 04:13 PM (7 years, 28 days ago) |
|
|
|
unlimitedmaster
grower
Registered: 12/31/16
Posts: 38
Last seen: 6 years, 3 months
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23976636 - 01/02/17 04:16 PM (7 years, 28 days ago) |
|
|
Ya could use a spoon to scale. With experience a spoon or cup is all ya need. U just need some practice. Borrow a scale to get used to it.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23976864 - 01/02/17 05:46 PM (7 years, 28 days ago) |
|
|
First off, I do read the damn cultivation pages and teks. Mushboy replies "30min of heat to cracked corn will give you one nasty mess". But he doesn't answer any of my questions. Why reply????? Your not going to explain your answer? Are you just trying to up your posting numbers. The freggin tek on this site for the preparation as cracked corn for bulk substrates specifically states: "1. Get the estimated amount of CC you want and put it into a large pot. Next cover the corn with water and set the heat between "medium" and "high". 2. Simmer the CC 30 - 35 minutes on the stove is enough time, however it depends on how much you are doing and how much water/corn you are working with etc.. (with small amounts the heat may need to be adjusted lower to prevent boil over) For large batches this time was correct.
I have been registered to this site just shy of 2 years. I have laterally read hundreds of teks and information in the cultivation section as well as other sections, I have read thousands of posts and threads to gain my information not to mention I have been successful in many adventures. I am a noob, no doubt and will never achieve the level of knowledge that RR, Roadkill and others have, but I just want you to know that I would not have asked a question unless I felt that they were specific enough that no more time needed spent on my part looking at posts for the answers. In regards to the 1st question Stamets states that "complex sugars are reccommeded to supplement agars to increase vitality by forcing them to extract the sugars from the base medium", (see Growing Gourmet and Medicinal Mushrooms, pg 87. Stamets also says, Sugars in General are essential for the healthy growth of Mycelium, id and recommend different complex sugars but is silent on this particular medium as to the application of more simpliar sugars, i.e honey. Dog food already contains complex sugars but little "simple sugars" and nutrients found in honey. Hence, it may be beneficial for the overall health of the mycelium, unless the dog food medium contains an exceeding amount of complex sugars, which the honey would cause the agar to become toxic. If small amounts of the honey provide no additional benefit, or too much it may make the agar toxic, then I have no need to experiment needlessly on my budget. If your goal was simply to gain "posting" numbers by referring me to the "cultivation section" of this site, then you've succeeded. Im not trying to be a dick, I'm just saying no one even tried to answer my questions.
In regards to my second question about the coffee water to the cracked corn, I have done an ample reading of grain preparation and found general cites to the addition of coffee water to prepping bulk grains, and their benefit but nothing specific as to absorption rates for cracked corn or the specific benefit is would give cracked corn, if any.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein] 1
#23976980 - 01/02/17 06:38 PM (7 years, 28 days ago) |
|
|
wow
corn is loaded with starch. if you add heat and can/will get really sticky and clumpy. you want a nice loose grain that freely moves around in a jar after prep.
the consistancy of cooked crack corn is well... fucking gross. WBS(wild bird seed) has a similar issue so the way I prep my wbs is just a 24hr soak.
however, in terms of choice of grain for various drawbacks, crackcorn is the worst. moisture content is difficult to get right, the grains are really large and they clump together.
$8 for 50lbs is a great deal. but id never ever use crackcorn ever(tried it once. no way)
as for the rest of your post...
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23977060 - 01/02/17 07:11 PM (7 years, 28 days ago) |
|
|
Sure you did, just because it was there. Regardless, thank you for caring enough to answer my question. I guess I'm on my own on the DFA and honey, but I will use what you said in my considerations.
|
wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23977330 - 01/02/17 09:07 PM (7 years, 28 days ago) |
|
|
Can you link to the tek youre using please?
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: wildernessjunkie]
#23977446 - 01/02/17 10:00 PM (7 years, 28 days ago) |
|
|
I add coffee to all my grains. Adding honey to DFA will just slow growth. Dog food has enough nutrition on its own.
|
Intelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23977452 - 01/02/17 10:03 PM (7 years, 28 days ago) |
|
|
Coffee and a half pinch of gypsum
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Intelligentxfruit]
#23977499 - 01/02/17 10:22 PM (7 years, 28 days ago) |
|
|
Adding coffee to grain soak is perfect. Cc is not shakeable. Do not use grain to grain or regular agar wedges for your jar. Make li if you want results.
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: tump]
#23977512 - 01/02/17 10:26 PM (7 years, 28 days ago) |
|
|
I saw a tek about cracked corn a long time ago. I think you can make it work but there is a trick to it (that I can't remember ). It would be my last grain choice aside frome oil grains like flax.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: wildernessjunkie]
#23978344 - 01/03/17 10:11 AM (7 years, 27 days ago) |
|
|
https://www.shroomery.org/9034/Cracked-Corn-Preparation-Technique
This is the prep for the corn tek on this site. I was just going to do it for the jars. As for the end substrate I will probably use the bucket tek or the elementary coir tek on Damien50/50's links. Corn was not the base substrate just the inoculation substrate. Unless their are problems with this??? I have 200lbs of corn for feed. That's why I was switching from rye berries to cracked corn. Unless u guys think it is not practical.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23978348 - 01/03/17 10:12 AM (7 years, 27 days ago) |
|
|
dude that tek is 14 years old. mycology and growing illegal drugs, is ever changing and never written in stone. hence ....
Quote:
bodhisatta said: The cultivation section of this website is full of old garbage. Good research is done on the forums where there's time tested proven methods
pretend the site shroomery.org does not exist. only shroomery.org/forums
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23978355 - 01/03/17 10:16 AM (7 years, 27 days ago) |
|
|
If you can manage to prrp iy without turning it into a sticky starchy mess, it will work fine. That is easier said than done though.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23978359 - 01/03/17 10:17 AM (7 years, 27 days ago) |
|
|
and in case you get upset that i just shat on you for my own post count...
Quote:
Mushenstein said: Corn was not the base substrate just the inoculation substrate.
right. thats called spawn. we know.
Quote:
Mushenstein said:I have 200lbs of corn for feed.
thats a lot of corn!!
Quote:
Mushenstein said:Unless u guys think it is not practical.
no it is not practical. stick with rye. or wbs. or oats.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23978366 - 01/03/17 10:21 AM (7 years, 27 days ago) |
|
|
I read a post, after sifting through volumes of information, where someone recommended that the cracked corn be sifted through a colander to separate the small pieces and the dust before it is put in water. This supposedly gets rid of the mush, unless its cooked too long.
It felt like I found the holy grail of answers when I stumbled upon this post.
I will try the coffee with it then. I guess I will leave the honey out of the dog food agar.
I got some Petri dishes for Christmas and was going to try these with the agar instead of glad minirounds. After reviewing the petri teks I am assuming I can not pour the agar into the dishes before I pc them? This may result in a mess? I don't have a flow hood so sterilizing everything separately and then putting things together in a sab, or glove box seems substantially risky. Any thoughts, or am I right about petri's being pc'd without agar in them.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23978371 - 01/03/17 10:24 AM (7 years, 27 days ago) |
|
|
pretend the site shroomery.org does not exist. only shroomery.org/forums
I'm finding this to be good advise.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23978373 - 01/03/17 10:24 AM (7 years, 27 days ago) |
|
|
glad mini rounds are agar. its how you do 'no pour'. its awesome.
you cant pc petris with media because they are so thin. i think it gets fucked up with water coming in the dishes or something. plus plastic ones will melt.
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23978397 - 01/03/17 10:33 AM (7 years, 27 days ago) |
|
|
Yeah you can't sterilise petris unless they are made of glass. If they are plastic make sure they are sterile. Non sterile plastic petries are useless. Pouring plates in a still air box is fine I've done it hundreds of times and never had a plate contam pre inoculation.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23978577 - 01/03/17 11:37 AM (7 years, 27 days ago) |
|
|
My petri's are glass. I guess I will give it a go ahead in the SAB and cross my fingers. **Question: I notice on the teks that the petri dishes are sealed with saran wrap after inoculation. Is this right? doesn't that cut off gas exchange and stull the growth? Am I looking at this wrong? If so, when do you wrap/seal them and for how long? This is after inoculation, right???
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23978592 - 01/03/17 11:42 AM (7 years, 27 days ago) |
|
|
Yes wrap them with saran wrap or pera film after inoculation. It won't hurt gas exchange.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23978641 - 01/03/17 11:59 AM (7 years, 27 days ago) |
|
|
One more thing, on liquid Culture experiments does it matter what the size and shape of the glass container is? I would like to use smaller more manageable containers with less liquid, but some don't seem to work out as well. If the ratio's are proportional to the original recipe would there be any impact on size, shape or volume of the container, or even on reducing the amount of total media to be inoculated? meaning if you cut the recipe down too far does it stop working?
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23978644 - 01/03/17 12:01 PM (7 years, 27 days ago) |
|
|
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23979088 - 01/03/17 03:04 PM (7 years, 27 days ago) |
|
|
Thanks for the references. I have already been through the first one before, the second link will get a good read tonight. The thing is I have already been somewhat successful with LC's in the past. I'm just fuking them up 3 ways from Wednesday, now, for some odd reason. Hence the LC Q's. I'll concentrate on agar for now.
Do you guys see any difference in using clear tubs as opposed to non-see though tubs? (with the top modified to see through) Not sure if clear would let in too much light?
How about size of tubs. Any threshold in size, height, depth, capacity one should stay away from?
BTW, thanks for the help.
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23979126 - 01/03/17 03:20 PM (7 years, 27 days ago) |
|
|
No such thing as too much light. I use 27qt, 66qt and 105qt tubs.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23979288 - 01/03/17 04:25 PM (7 years, 27 days ago) |
|
|
Wow. 105 at. Hell if a tub. I guess everything is with the same parameters, just more sub.
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23979488 - 01/03/17 05:39 PM (7 years, 27 days ago) |
|
|
Yup. Brick and a half of coir, 2 qts verm and 9 qts of spawn.
 Harvesting them can be a bit much somtimes.

The 27qt are my favorite though.
Edited by MudaFuka (01/03/17 05:46 PM)
|
Mtez44
LuckyRabbit



Registered: 06/02/16
Posts: 448
Last seen: 1 year, 8 months
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23979546 - 01/03/17 05:53 PM (7 years, 27 days ago) |
|
|
I use glass petri's sterilized in PC and do all the work in SAB with no problems.
2 level teaspoons of agar powder, 2 tablespoons of potato flakes, drop of honey and a cup of water all in a pan on low/medium heat. Does 4-6 petri's. Probably more if you keep them thin
It was someone else's tek but I can't remember who. The water can be more/less depending on consistency desired. I personally use a full cup.
You were looking for agar recipies that were not based in weight so I hope this helps
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mtez44]
#23980752 - 01/04/17 08:07 AM (7 years, 26 days ago) |
|
|
Ill give it a try. I'm trying to make dog food agar, but Ill give that PDA recipe a shot as well.
That just seems like a lot of exposure for petri's in an SAB, but I'm willing to take the chance since I have the Petri's.
Thanks.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23980757 - 01/04/17 08:09 AM (7 years, 26 days ago) |
|
|
Do you use any poo, coffee or earth worm castings in your recipe? I've heard it helps out in some respects but you have to be careful with contam. Ever try throwing a handful in your recipes???
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23980776 - 01/04/17 08:25 AM (7 years, 26 days ago) |
|
|
Sure we have. But coir/verm is the only safe nonn pasteurization sub. You can make all the sub you want with proper pasteurization but effort is 10 fold
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: tump]
#23981023 - 01/04/17 10:29 AM (7 years, 26 days ago) |
|
|
Wait a minute, wait a minute, wait a minute. .....Your telling me you can do a non-pasteurized bulk substrate with Coir and Verm????? You at least do a bucket tek where you dump in boiling water, Right? I was, and still am under the impression that all substrates need pasteurization of some sort, i.e. bucket tek.
Right????
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23981030 - 01/04/17 10:32 AM (7 years, 26 days ago) |
|
|
tump ment cvg can be sterilized. any poo based subs can only be pasteurized.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23981690 - 01/04/17 03:09 PM (7 years, 26 days ago) |
|
|
Ok, I am understanding the post a little better. I am still a little astonished. I did not know you could sterilize a straight CVG substrate and yield fruits without horrific contam results. Would this be the same proportions of CVG we talked about just put in a oven bag and pc'd for 90 min on 15psi? Let cool and mix with spawn in a tub and black bag it for 10 days?? Or are their some risks with contam?
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23981696 - 01/04/17 03:13 PM (7 years, 26 days ago) |
|
|
MudaFuka, did you case that tub? those are some damn good results, without much room in between the fruits.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23981744 - 01/04/17 03:38 PM (7 years, 26 days ago) |
|
|
CVG can be hydrated with tap water Or the bucket TEK Or pasteurization Or sterilization
It doesn't matter. But heat treatment makes it perform better. So i advocate bucket TEK or sterilization and only would suggest pasteurization of coir as a learning experience.
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23981878 - 01/04/17 04:36 PM (7 years, 26 days ago) |
|
|
I rairly case bulk subs. I generally just add a thick top layer of CVG at spawning. I don't add thing like coffee or castings to conventional bulk subs either. I save that kind of thing for bottle subs that get sterilised and stay sterile untill fruiting.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23982178 - 01/04/17 06:26 PM (7 years, 26 days ago) |
|
|
Do you think poo or coffee adds potency to the end product or is it just a health and nutrient thing for the fruit to be bigger or flush better?
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23982193 - 01/04/17 06:31 PM (7 years, 26 days ago) |
|
|
bod, did you case any of the tubs you have in your signature? I read all of these threads about casing and performance. In my limited experience I didn't use it and things went fine. Is there really a benefit of casing, and if so what is it actually?
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23982245 - 01/04/17 06:52 PM (7 years, 26 days ago) |
|
|
No I rarely case if I do it's a bit of verm or leftover CVG Potency is genetics the substrate plays no noticeable role in that. Our substrates mixed with spawn are often overly nutritional anyway. With water being the weak link to get all the potential out of the grow.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23982418 - 01/04/17 08:09 PM (7 years, 26 days ago) |
|
|
Then Im not understanding. If coir, verm, gypsum creates fantastic flushed, which it does, why do people waste their time with poo and high nitro supplements/substrates?
Same with casing, seems it just doesn't have that mush of an effect save for adding a bit of water. Why waste the time when the difference is negligible?
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23982436 - 01/04/17 08:17 PM (7 years, 26 days ago) |
|
|
I don't know. I used to add coffee and shit because I thought it would make a big difference. After I figured out how little it really helped. I stopped doing it.
Casing for cubes doesn't really improve the grow. It just makes it easier to maintain good conditions.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23983308 - 01/05/17 08:20 AM (7 years, 25 days ago) |
|
|
You just said a million words in three sentences. Its unfortunate no one else chimed in to give their support or feed back for "casing" and the use of "high nitrogen" supplements such as poo and coffee substrates as opposed to non cased tubs and coir/verm substrates.
Would you say that with caseing and/or poo/coffee substrates you would get more then 3 flushes like you get with coir/verm/gypsum???
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23983543 - 01/05/17 10:26 AM (7 years, 25 days ago) |
|
|
I've gone for 6 flushes with CVG. Moste of the nutrition is in the grain spawn. If you want more nuts for later flushes. Use more spawn.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23983558 - 01/05/17 10:32 AM (7 years, 25 days ago) |
|
|
Quote:
Mushenstein said: Its unfortunate no one else chimed in to give their support or feed back for "casing" and the use of "high nitrogen" supplements such as poo and coffee substrates as opposed to non cased tubs and coir/verm substrates.
Would you say that with caseing and/or poo/coffee substrates you would get more then 3 flushes like you get with coir/verm/gypsum???
Probably because you were stubborn on page one with the stuff you found yourself lol. You'll find people that say all different things here too. You need to do your research too.
Some people use poo because they get it free. Some people are religiously dogmatic and think poo does a better job but can't seem to prove it ever...
|
pblcenmy1
str8 noob



Registered: 11/24/09
Posts: 221
Loc: Colo. Rockies
Last seen: 7 years, 23 days
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23983605 - 01/05/17 10:58 AM (7 years, 25 days ago) |
|
|
The wonder of coffee grounds revolves around its overall ph and carbon nitrogen. Not just nitrogen. Too high a ratio and nutrients slow to mineralize and uptake. Too low a ratio and bacteria spread like wildfire. Coffee i believe is 30:1
-------------------- ~LoveLife~ Down 2 Earth, Up N Space!
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23983616 - 01/05/17 11:05 AM (7 years, 25 days ago) |
|
|
I can't say that I've heard that from anyone, but your logic is sound.
It is the same with LC's and DF agar. Adding potato water would help with overall health and longevity? Or is it better to stay with the original recipes?
I was talking about adding like a teaspoon of potato water, not a whole soak in the original water.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: pblcenmy1]
#23983618 - 01/05/17 11:07 AM (7 years, 25 days ago) |
|
|
Quote:
pblcenmy1 said: Too low a ratio and bacteria spread like wildfire. Coffee i believe is 30:1
Can you elaborate on this. Pretend I'm an idiot. Which isn't too far off.
|
pblcenmy1
str8 noob



Registered: 11/24/09
Posts: 221
Loc: Colo. Rockies
Last seen: 7 years, 23 days
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23983630 - 01/05/17 11:10 AM (7 years, 25 days ago) |
|
|
Potatoes are starchy. Use a yam
With the ratio consider carbon the dry areas of substrate and nitrogen being the wet. Too much wet and its a bacteria soup. Too dry and nothing really happens until the balance gets near medium.
-------------------- ~LoveLife~ Down 2 Earth, Up N Space!
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: pblcenmy1]
#23984723 - 01/05/17 06:04 PM (7 years, 25 days ago) |
|
|
Well, I bought a yam tonight on the way home from work. Who woulda figured the little store would have a yam....Ha. I felt ridiculous putting a yam up on the counter. The cashier even laughed. .45 cents.
By the way I have done a lot of reading about casings and its hard to find any consistency. I will have to do my own trials and errors after I get this agar down. I did find a hell of a way to do petri's and let them sit until the end of time sterilized, until I want to use them.
Vacuum seal and then PC. Leave in the the vacuum seal and they are forever sterilized until use. At least I think that's how they work.
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23984784 - 01/05/17 06:28 PM (7 years, 25 days ago) |
|
|
Are those vacuume bags autoclave able?
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23984832 - 01/05/17 06:47 PM (7 years, 25 days ago) |
|
|
You bet. bought the ones right out of Wal-Mart that go with the vacuum seal machine. PC'd one last night with a syringe in it to see if the seal would break. Pc'd at 14 psi for over an hour. Seal is intact. No leaks, No damage to the bag or the syringe. Unfortunately, I bought the "power pressure cooker" advertised on late night tv with the sham wow and the slap chop. But it still does the trick at 14 psi, I just set it for an extra 10-15 minutes when I pc something. But hell yeah. Petris, syringes and everything else can sit in the bags vacuum tight and sterile until I decide to use it.
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23985146 - 01/05/17 08:50 PM (7 years, 25 days ago) |
|
|
Nice.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23985354 - 01/05/17 10:16 PM (7 years, 25 days ago) |
|
|
Update: A few eggs must be broken in the process of every quasi new idea. I broke a small petri after it was vacuum sealed. The vacuum was a little to tight. Note to self, press the seal button on the vacuum sealer before the vacuum gets ungodly tight and breaks the petri dish. Of course these are glass (flint) petri's and the larger ones didn't have this problem, but it just breaks my heart to hear a flint petri go POP! So be forewarned if you try this.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23985367 - 01/05/17 10:22 PM (7 years, 25 days ago) |
|
|
could you vacuum seal a wedge and freeze it? or does that kill the culture?
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23985373 - 01/05/17 10:24 PM (7 years, 25 days ago) |
|
|
Yeaaaaahhhh. Don't do that.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23985374 - 01/05/17 10:24 PM (7 years, 25 days ago) |
|
|
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23986172 - 01/06/17 10:12 AM (7 years, 24 days ago) |
|
|
Hard to say, but it is a good question. The vacuum seal bags should be sterilized right out of the box, but I would still wipe down with 91% alcohol before sealing. 91% because it evaporates quickly. The cold should do nothing to the myc. I have seen spore syringes frozen at sub 0 temps for months and were still viable upon DE thawing.
My only concern would be the atmospheric pressure in the bag. My guess would be that it would survive to use again. The only way to find out is to do it. When I finish my agar Petri's I will let you know.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23987914 - 01/06/17 09:55 PM (7 years, 24 days ago) |
|
|
I'm doing dog food agar and MEA in Snapple bottles for containers. Fortunately I found a TEK that is right on point, but it doesn't mention if I can leave the agar pc'd in the Snapple bottle and just PC the agar each time I want to use it. There are some teks that say you can warm it up each time to over 185F and it becomes liquid again. Why not just pc it again and not worry about it cause any new contams would be dead and it will be liquid again. (that is to say if it got contams in it they would probably grow into something nasty in a full bottle of agar)
This leads me to my second question. Some teks say you need a hole in your lid with a filter on it with tin foil over that. Others say there is no need for a modified lid. Which is it??? I don't want the Snapple bottle to explode in the PC causing all kinds of mess and wasted agar, but if I don't have to put a hole in it I sure as hell am not.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23988103 - 01/06/17 11:09 PM (7 years, 24 days ago) |
|
|
1: too much pc time and you run the risk of camelization 2: a vacuum would be more of a risk
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: cronicr]
#23988911 - 01/07/17 09:28 AM (7 years, 23 days ago) |
|
|
1. I was unaware that DFA would caramelize. So I should only PC the DFA one time and there after either use it the first time or throw it out?????
Is it the Dog Food that caramelizes or the agar?
2. The Snapple bottle without a hole and filter would cause a vacuum. I believe this is 100% correct, but as long as the bottle doesn't break and I can manage to open it should be OK, right?
Or is the issue when I open the bottle the vacuum causes air to be sucked into the bottle increasing contamination possibilities?
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23988930 - 01/07/17 09:33 AM (7 years, 23 days ago) |
|
|
Sugar is the only thing that can caramelize.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: cronicr]
#23988974 - 01/07/17 09:50 AM (7 years, 23 days ago) |
|
|
Quote:
bodhisatta said: Sugar is the only thing that can caramelize.
Quote:
cronicr said: 1: too much pc time and you run the risk of camelization 2: a vacuum would be more of a risk
What the Hell is he talking about then?
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23988994 - 01/07/17 09:59 AM (7 years, 23 days ago) |
|
|
The sugars caramelizing
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23989974 - 01/07/17 04:06 PM (7 years, 23 days ago) |
|
|
Should you wrap the petri's with syran wrap and seal them after you pour the agar or is it ok to just bag them until they solidify? The posts I've read go both ways.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23990000 - 01/07/17 04:13 PM (7 years, 23 days ago) |
|
|
I let them solidify then wrap or bag.
if you pour your agar at the correct temperature range it will take under 10-15 minutes to solidify
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990329 - 01/07/17 05:57 PM (7 years, 23 days ago) |
|
|
As big of a pain in the ass that this "power pressure cooker" is that I bought off an infomercial, one nice redeeming quality is that it goes automatically to "warm" when the Pressure Cook has completed the selected cycle. The "warm" setting remains on as long as you leave it on, so my agar never gets cold and never solidifies as long as it in the pressure cooker. The "Warm" setting is approximately 170F.....if my meat thermometer is accurate. 
How cold does it have to be to inoculate? If your saying 10-15 minutes, then I will just leave it in the glove box and "knock it up" in a little bit
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23990335 - 01/07/17 05:59 PM (7 years, 23 days ago) |
|
|
https://www.shroomery.org/forums/showflat.php/Number/21922023
Quote:
Agar exhibits hysteresis, melting at 85 °C (358 K, 185 °F) and solidifying from 32–40 °C (305–313 K, 90–104 °F). This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures.
Quote:
I find it starts to gel back up at about 107F or 42C most of the time at the gel strength used for plates. at 47C you can pour a whole sleeve of plates before it gels up on you. and pouring at as low of a temperature as possible minimizes condensation a lot.
to get agar to melt again you have to go all the way back up to 85C, once it is gel simply heating it 47+ won't melt it.
pour your agar a little less than 10F or so above the solidification temperature like 115-117 or so or 46-47C
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990763 - 01/07/17 08:09 PM (7 years, 23 days ago) |
|
|

Well Fuk, that didn't work out too well. I made a new glove box, above. Got tired of using a SAB. But I put the Dog Food Agar in at 170 and waited 20 minutes and it was chunky. Like Turd Jello or something my dog shit out. Note to self, Pour in 10 minutes as opposed to 20.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23990776 - 01/07/17 08:13 PM (7 years, 23 days ago) |
|
|
Quote:
Mushenstein said:


that aint going to work well should look like this

gloves go on your hands
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990906 - 01/07/17 08:55 PM (7 years, 23 days ago) |
|
|
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990947 - 01/07/17 09:05 PM (7 years, 23 days ago) |
|
|
Yeah my glove box looks like someone with Palsy made it. It was a bit small for the work I was doing, but it was damn near air tight. The agar really got fuk'd. The box, well I need to try a few smaller sized inoc's before I toss it. Put a lot of time into it.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23990969 - 01/07/17 09:11 PM (7 years, 23 days ago) |
|
|
yea the problem is you don't want attached sleeves or gloves or the air currents they create. may as well have no box at all. you cannot sanitize or sterilize the inside of the SAB so you need to work with physics not the chemicals you spray or wipe
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990998 - 01/07/17 09:22 PM (7 years, 23 days ago) |
|
|
Damn, I really thought it was a good idea, I tried to seal it all the way. So in your SAB the petri's are just sitting there, unwrapped. Aren't you worried about contam. I mean shouldn't they at least be in a zip lock or in tin foil until you innoc. them?
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23991014 - 01/07/17 09:28 PM (7 years, 23 days ago) |
|
|
they're fine. & they got wrapped
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23991059 - 01/07/17 09:45 PM (7 years, 23 days ago) |
|
|
Were those ones knocked' up or were they just poured and cooling down?
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23991106 - 01/07/17 10:00 PM (7 years, 23 days ago) |
|
|
cooling down, they have to cool down unwrapped just sitting in a SAB it's fine they're fine
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23992086 - 01/08/17 10:44 AM (7 years, 22 days ago) |
|
|
Oh! I guess that will let the condensation out. If they are wrapped or bagged on the cool down there is probably condensation during the inoculation. I poured and wrapped back into the tin foil they were already in, then I let them cool down. Probably a bad idea. I'll let you know how it turns out. I am doing a comprehensive Cracked Corn prep. while I was waiting. Since all of the info on here seems old and outdated for Crack Corn I figured I would test out several different spawn preps and see which one works.
I know a lot of people swear by DFA and Many swear by MEA. Do you have a preference and why?? I have the ingredients for both.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23992274 - 01/08/17 12:21 PM (7 years, 22 days ago) |
|
|
Grain soak water > mea > pda > YPD
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23992533 - 01/08/17 02:07 PM (7 years, 22 days ago) |
|
|
Your saying soak grains in water and then use the water for agar recipe???
Also, MEA = Malt Extract Agar, PDA = Potato Dextrose Agar, What is YPD???
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23992734 - 01/08/17 03:26 PM (7 years, 22 days ago) |
|
|
Y stands for yeast
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23992944 - 01/08/17 04:25 PM (7 years, 22 days ago) |
|
|
Quote:
Mushenstein said: Your saying soak grains in water and then use the water for agar recipe???
Also, MEA = Malt Extract Agar, PDA = Potato Dextrose Agar, What is YPD???
Yeast peptone dextrose. Usually lab made. And just put a bowl under your strainer and save that super useful grain water
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23992997 - 01/08/17 04:42 PM (7 years, 22 days ago) |
|
|
I'm into making it now, maybe buy agar when I quit being cheap. I do save grain water(s). Potato, Yam, spent coffee grains, egg shells, wood soaked water. I save everything in mason jars, PC it and leave it in the foil until I'm going to use it. If its sterilized it seems to last a good while.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23993247 - 01/08/17 06:20 PM (7 years, 22 days ago) |
|
|
any other applications you could use grain water for? im sure using it in your cvg/bulk would not be a good idea... although....
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23993311 - 01/08/17 06:37 PM (7 years, 22 days ago) |
|
|
Makes great LC, agar, hydration water for bottle tek.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23993326 - 01/08/17 06:40 PM (7 years, 22 days ago) |
|
|
grain water lc you say?
my birdseed water always smells semi fermented after a 24hr soak.. maybe scoop out halfway through?
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23993346 - 01/08/17 06:45 PM (7 years, 22 days ago) |
|
|
I don't use any grain that I can't from dry to hydrated and then sterilized in an 5-6 hour span.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23993355 - 01/08/17 06:47 PM (7 years, 22 days ago) |
|
|
okie dokie.
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23993610 - 01/08/17 08:05 PM (7 years, 22 days ago) |
|
|
Why not use it in bulk? If you have to get to field capacity anyways why not sterilize some of it and add it in with your other water at say a 10-20% Grain Water to Water ratio? Maybe not full-on 100%grain, but a smaller % of it should help the bulk. Especially if its the same type of grain water that you used in your agar, then in the spawn. The myc can get nutrient preferential if the same grain water is used over and over again to clean up a strain on agar.
Is it the possibility of contamination if the grain water is used in the preparation, field capacity or pasteurization of the bulk?
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23993670 - 01/08/17 08:21 PM (7 years, 22 days ago) |
|
|
I would be surprised if it didn't contaminate if you used it in bulk substrate
|
Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
|
Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23993763 - 01/08/17 08:50 PM (7 years, 22 days ago) |
|
|
Here we go. I just finished knock'n um up. The dishes really look like I fed my dog 3 month old sushi and followed him around with petri's. Hope it doesn't effect the appetite of the myc/spores.
The small Petri's were a split. 2 with agar wedges from my pasty plates and one was spore solution.

The large Petri's were also a split. 1 with spore solution and 2 with a print and a homemade loop.

Some of the dishes had condensation on the lid. Unfortunate, but I don't think I can fix it now there knocked up.
|
|