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InvisibleMushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
    #23992086 - 01/08/17 10:44 AM (7 years, 22 days ago)

Oh! I guess that will let the condensation out. If they are wrapped or bagged on the cool down there is probably condensation during the inoculation.
I poured and wrapped back into the tin foil they were already in, then I let them cool down. Probably a bad idea. I'll let you know how it turns out. I am doing a comprehensive Cracked Corn prep. while I was waiting. Since all of the info on here seems old and outdated for Crack Corn I figured I would test out several different spawn preps and see which one works.

I know a lot of people swear by DFA and Many swear by MEA. Do you have a preference and why?? I have the ingredients for both.


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InvisiblebodhisattaMDiscordReddit
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
    #23992274 - 01/08/17 12:21 PM (7 years, 22 days ago)

Grain soak water > mea > pda > YPD


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InvisibleMushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
    #23992533 - 01/08/17 02:07 PM (7 years, 22 days ago)

Your saying soak grains in water and then use the water for agar recipe???

Also, MEA = Malt Extract Agar,  PDA = Potato Dextrose Agar, What is YPD???


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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
    #23992734 - 01/08/17 03:26 PM (7 years, 22 days ago)

Y stands for yeast


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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
    #23992944 - 01/08/17 04:25 PM (7 years, 22 days ago)

Quote:

Mushenstein said:
Your saying soak grains in water and then use the water for agar recipe???

Also, MEA = Malt Extract Agar,  PDA = Potato Dextrose Agar, What is YPD???



Yeast peptone dextrose. Usually lab made.
And just put a bowl under your strainer and save that super useful grain water


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InvisibleMushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
    #23992997 - 01/08/17 04:42 PM (7 years, 22 days ago)

I'm into making it now, maybe buy agar when I quit being cheap. I do save grain water(s). Potato, Yam, spent coffee grains, egg shells, wood soaked water. I save everything in mason jars, PC it and leave it in the foil until I'm going to use it. If its sterilized it seems to last a good while.


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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
    #23993247 - 01/08/17 06:20 PM (7 years, 22 days ago)

any other applications you could use grain water for? im sure using it in your cvg/bulk would not be a good idea... although....


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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
    #23993311 - 01/08/17 06:37 PM (7 years, 22 days ago)

Makes great LC, agar, hydration water for bottle tek.


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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
    #23993326 - 01/08/17 06:40 PM (7 years, 22 days ago)

grain water lc you say?

my birdseed water always smells semi fermented after a 24hr soak.. maybe scoop out halfway through?


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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
    #23993346 - 01/08/17 06:45 PM (7 years, 22 days ago)

I don't use any grain that I can't from dry to hydrated and then sterilized in an 5-6 hour span.


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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
    #23993355 - 01/08/17 06:47 PM (7 years, 22 days ago)

:mostinteresting:

okie dokie.


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InvisibleMushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
    #23993610 - 01/08/17 08:05 PM (7 years, 22 days ago)

Why not use it in bulk? If you have to get to field capacity anyways why not sterilize some of it and add it in with your other water at say a 10-20% Grain Water to Water ratio? Maybe not full-on 100%grain, but a smaller % of it should help the bulk.
Especially if its the same type of grain water that you used in your agar, then in the spawn. The myc can get nutrient preferential if the same grain water is used over and over again to clean up a strain on agar. 

Is it the possibility of contamination if the grain water is used in the preparation, field capacity or pasteurization of the bulk?


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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
    #23993670 - 01/08/17 08:21 PM (7 years, 22 days ago)

I would be surprised if it didn't contaminate if you used it in bulk substrate


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InvisibleMushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
    #23993763 - 01/08/17 08:50 PM (7 years, 22 days ago)

Here we go. I just finished knock'n um up. The dishes really look like I fed my dog 3 month old sushi and followed him around with petri's. Hope it doesn't effect the appetite of the myc/spores.

The small Petri's were a split. 2 with agar wedges from my pasty plates and one was spore solution.



The large Petri's were also a split. 1 with spore solution and 2 with a print and a homemade loop.



Some of the dishes had condensation on the lid. Unfortunate, but I don't think I can fix it now there knocked up.



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