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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23986172 - 01/06/17 10:12 AM (7 years, 24 days ago) |
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Hard to say, but it is a good question. The vacuum seal bags should be sterilized right out of the box, but I would still wipe down with 91% alcohol before sealing. 91% because it evaporates quickly. The cold should do nothing to the myc. I have seen spore syringes frozen at sub 0 temps for months and were still viable upon DE thawing.
My only concern would be the atmospheric pressure in the bag. My guess would be that it would survive to use again. The only way to find out is to do it. When I finish my agar Petri's I will let you know.
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23987914 - 01/06/17 09:55 PM (7 years, 24 days ago) |
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I'm doing dog food agar and MEA in Snapple bottles for containers. Fortunately I found a TEK that is right on point, but it doesn't mention if I can leave the agar pc'd in the Snapple bottle and just PC the agar each time I want to use it. There are some teks that say you can warm it up each time to over 185F and it becomes liquid again. Why not just pc it again and not worry about it cause any new contams would be dead and it will be liquid again. (that is to say if it got contams in it they would probably grow into something nasty in a full bottle of agar)
This leads me to my second question. Some teks say you need a hole in your lid with a filter on it with tin foil over that. Others say there is no need for a modified lid. Which is it??? I don't want the Snapple bottle to explode in the PC causing all kinds of mess and wasted agar, but if I don't have to put a hole in it I sure as hell am not.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23988103 - 01/06/17 11:09 PM (7 years, 23 days ago) |
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1: too much pc time and you run the risk of camelization 2: a vacuum would be more of a risk
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: cronicr]
#23988911 - 01/07/17 09:28 AM (7 years, 23 days ago) |
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1. I was unaware that DFA would caramelize. So I should only PC the DFA one time and there after either use it the first time or throw it out?????
Is it the Dog Food that caramelizes or the agar?
2. The Snapple bottle without a hole and filter would cause a vacuum. I believe this is 100% correct, but as long as the bottle doesn't break and I can manage to open it should be OK, right?
Or is the issue when I open the bottle the vacuum causes air to be sucked into the bottle increasing contamination possibilities?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23988930 - 01/07/17 09:33 AM (7 years, 23 days ago) |
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Sugar is the only thing that can caramelize.
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: cronicr]
#23988974 - 01/07/17 09:50 AM (7 years, 23 days ago) |
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Quote:
bodhisatta said: Sugar is the only thing that can caramelize.
Quote:
cronicr said: 1: too much pc time and you run the risk of camelization 2: a vacuum would be more of a risk
What the Hell is he talking about then?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23988994 - 01/07/17 09:59 AM (7 years, 23 days ago) |
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The sugars caramelizing
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23989974 - 01/07/17 04:06 PM (7 years, 23 days ago) |
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Should you wrap the petri's with syran wrap and seal them after you pour the agar or is it ok to just bag them until they solidify? The posts I've read go both ways.
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bodhisatta 
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Registered: 04/30/13
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23990000 - 01/07/17 04:13 PM (7 years, 23 days ago) |
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I let them solidify then wrap or bag.
if you pour your agar at the correct temperature range it will take under 10-15 minutes to solidify
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990329 - 01/07/17 05:57 PM (7 years, 23 days ago) |
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As big of a pain in the ass that this "power pressure cooker" is that I bought off an infomercial, one nice redeeming quality is that it goes automatically to "warm" when the Pressure Cook has completed the selected cycle. The "warm" setting remains on as long as you leave it on, so my agar never gets cold and never solidifies as long as it in the pressure cooker. The "Warm" setting is approximately 170F.....if my meat thermometer is accurate. 
How cold does it have to be to inoculate? If your saying 10-15 minutes, then I will just leave it in the glove box and "knock it up" in a little bit
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bodhisatta 
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Registered: 04/30/13
Posts: 61,889
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23990335 - 01/07/17 05:59 PM (7 years, 23 days ago) |
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https://www.shroomery.org/forums/showflat.php/Number/21922023
Quote:
Agar exhibits hysteresis, melting at 85 °C (358 K, 185 °F) and solidifying from 32–40 °C (305–313 K, 90–104 °F). This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures.
Quote:
I find it starts to gel back up at about 107F or 42C most of the time at the gel strength used for plates. at 47C you can pour a whole sleeve of plates before it gels up on you. and pouring at as low of a temperature as possible minimizes condensation a lot.
to get agar to melt again you have to go all the way back up to 85C, once it is gel simply heating it 47+ won't melt it.
pour your agar a little less than 10F or so above the solidification temperature like 115-117 or so or 46-47C
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990763 - 01/07/17 08:09 PM (7 years, 23 days ago) |
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Well Fuk, that didn't work out too well. I made a new glove box, above. Got tired of using a SAB. But I put the Dog Food Agar in at 170 and waited 20 minutes and it was chunky. Like Turd Jello or something my dog shit out. Note to self, Pour in 10 minutes as opposed to 20.
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bodhisatta 
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Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23990776 - 01/07/17 08:13 PM (7 years, 23 days ago) |
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Quote:
Mushenstein said:


that aint going to work well should look like this

gloves go on your hands
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990906 - 01/07/17 08:55 PM (7 years, 23 days ago) |
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990947 - 01/07/17 09:05 PM (7 years, 23 days ago) |
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Yeah my glove box looks like someone with Palsy made it. It was a bit small for the work I was doing, but it was damn near air tight. The agar really got fuk'd. The box, well I need to try a few smaller sized inoc's before I toss it. Put a lot of time into it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23990969 - 01/07/17 09:11 PM (7 years, 23 days ago) |
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yea the problem is you don't want attached sleeves or gloves or the air currents they create. may as well have no box at all. you cannot sanitize or sterilize the inside of the SAB so you need to work with physics not the chemicals you spray or wipe
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23990998 - 01/07/17 09:22 PM (7 years, 23 days ago) |
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Damn, I really thought it was a good idea, I tried to seal it all the way. So in your SAB the petri's are just sitting there, unwrapped. Aren't you worried about contam. I mean shouldn't they at least be in a zip lock or in tin foil until you innoc. them?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23991014 - 01/07/17 09:28 PM (7 years, 23 days ago) |
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they're fine. & they got wrapped
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Mushenstein
Brain Damage



Registered: 02/19/15
Posts: 680
Loc: At The Gates
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23991059 - 01/07/17 09:45 PM (7 years, 23 days ago) |
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Were those ones knocked' up or were they just poured and cooling down?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23991106 - 01/07/17 10:00 PM (7 years, 23 days ago) |
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cooling down, they have to cool down unwrapped just sitting in a SAB it's fine they're fine
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