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mushboy
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23978373 - 01/03/17 10:24 AM (7 years, 27 days ago) |
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glad mini rounds are agar. its how you do 'no pour'. its awesome.
you cant pc petris with media because they are so thin. i think it gets fucked up with water coming in the dishes or something. plus plastic ones will melt.
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MudaFuka
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Registered: 12/14/13
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23978397 - 01/03/17 10:33 AM (7 years, 27 days ago) |
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Yeah you can't sterilise petris unless they are made of glass. If they are plastic make sure they are sterile. Non sterile plastic petries are useless. Pouring plates in a still air box is fine I've done it hundreds of times and never had a plate contam pre inoculation.
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Mushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23978577 - 01/03/17 11:37 AM (7 years, 27 days ago) |
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My petri's are glass. I guess I will give it a go ahead in the SAB and cross my fingers. **Question: I notice on the teks that the petri dishes are sealed with saran wrap after inoculation. Is this right? doesn't that cut off gas exchange and stull the growth? Am I looking at this wrong? If so, when do you wrap/seal them and for how long? This is after inoculation, right???
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MudaFuka
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23978592 - 01/03/17 11:42 AM (7 years, 27 days ago) |
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Yes wrap them with saran wrap or pera film after inoculation. It won't hurt gas exchange.
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Mushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23978641 - 01/03/17 11:59 AM (7 years, 27 days ago) |
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One more thing, on liquid Culture experiments does it matter what the size and shape of the glass container is? I would like to use smaller more manageable containers with less liquid, but some don't seem to work out as well. If the ratio's are proportional to the original recipe would there be any impact on size, shape or volume of the container, or even on reducing the amount of total media to be inoculated? meaning if you cut the recipe down too far does it stop working?
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bodhisatta 
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Registered: 04/30/13
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23978644 - 01/03/17 12:01 PM (7 years, 27 days ago) |
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Mushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23979088 - 01/03/17 03:04 PM (7 years, 27 days ago) |
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Thanks for the references. I have already been through the first one before, the second link will get a good read tonight. The thing is I have already been somewhat successful with LC's in the past. I'm just fuking them up 3 ways from Wednesday, now, for some odd reason. Hence the LC Q's. I'll concentrate on agar for now.
Do you guys see any difference in using clear tubs as opposed to non-see though tubs? (with the top modified to see through) Not sure if clear would let in too much light?
How about size of tubs. Any threshold in size, height, depth, capacity one should stay away from?
BTW, thanks for the help.
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MudaFuka
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23979126 - 01/03/17 03:20 PM (7 years, 27 days ago) |
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No such thing as too much light. I use 27qt, 66qt and 105qt tubs.
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Mushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23979288 - 01/03/17 04:25 PM (7 years, 27 days ago) |
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Wow. 105 at. Hell if a tub. I guess everything is with the same parameters, just more sub.
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MudaFuka
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Registered: 12/14/13
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23979488 - 01/03/17 05:39 PM (7 years, 27 days ago) |
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Yup. Brick and a half of coir, 2 qts verm and 9 qts of spawn.
 Harvesting them can be a bit much somtimes.

The 27qt are my favorite though.
Edited by MudaFuka (01/03/17 05:46 PM)
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Mtez44
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23979546 - 01/03/17 05:53 PM (7 years, 27 days ago) |
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I use glass petri's sterilized in PC and do all the work in SAB with no problems.
2 level teaspoons of agar powder, 2 tablespoons of potato flakes, drop of honey and a cup of water all in a pan on low/medium heat. Does 4-6 petri's. Probably more if you keep them thin
It was someone else's tek but I can't remember who. The water can be more/less depending on consistency desired. I personally use a full cup.
You were looking for agar recipies that were not based in weight so I hope this helps
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Mushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mtez44]
#23980752 - 01/04/17 08:07 AM (7 years, 26 days ago) |
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Ill give it a try. I'm trying to make dog food agar, but Ill give that PDA recipe a shot as well.
That just seems like a lot of exposure for petri's in an SAB, but I'm willing to take the chance since I have the Petri's.
Thanks.
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Mushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23980757 - 01/04/17 08:09 AM (7 years, 26 days ago) |
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Do you use any poo, coffee or earth worm castings in your recipe? I've heard it helps out in some respects but you have to be careful with contam. Ever try throwing a handful in your recipes???
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tump
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23980776 - 01/04/17 08:25 AM (7 years, 26 days ago) |
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Sure we have. But coir/verm is the only safe nonn pasteurization sub. You can make all the sub you want with proper pasteurization but effort is 10 fold
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Mushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: tump]
#23981023 - 01/04/17 10:29 AM (7 years, 26 days ago) |
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Wait a minute, wait a minute, wait a minute. .....Your telling me you can do a non-pasteurized bulk substrate with Coir and Verm????? You at least do a bucket tek where you dump in boiling water, Right? I was, and still am under the impression that all substrates need pasteurization of some sort, i.e. bucket tek.
Right????
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mushboy
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23981030 - 01/04/17 10:32 AM (7 years, 26 days ago) |
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tump ment cvg can be sterilized. any poo based subs can only be pasteurized.
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Mushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: mushboy]
#23981690 - 01/04/17 03:09 PM (7 years, 26 days ago) |
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Ok, I am understanding the post a little better. I am still a little astonished. I did not know you could sterilize a straight CVG substrate and yield fruits without horrific contam results. Would this be the same proportions of CVG we talked about just put in a oven bag and pc'd for 90 min on 15psi? Let cool and mix with spawn in a tub and black bag it for 10 days?? Or are their some risks with contam?
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Mushenstein
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: MudaFuka]
#23981696 - 01/04/17 03:13 PM (7 years, 26 days ago) |
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MudaFuka, did you case that tub? those are some damn good results, without much room in between the fruits.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: Mushenstein]
#23981744 - 01/04/17 03:38 PM (7 years, 26 days ago) |
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CVG can be hydrated with tap water Or the bucket TEK Or pasteurization Or sterilization
It doesn't matter. But heat treatment makes it perform better. So i advocate bucket TEK or sterilization and only would suggest pasteurization of coir as a learning experience.
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MudaFuka
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Registered: 12/14/13
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Re: Any Benefit to adding Coffee to CC and Honey to DFA [Re: bodhisatta]
#23981878 - 01/04/17 04:36 PM (7 years, 26 days ago) |
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I rairly case bulk subs. I generally just add a thick top layer of CVG at spawning. I don't add thing like coffee or castings to conventional bulk subs either. I save that kind of thing for bottle subs that get sterilised and stay sterile untill fruiting.
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