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Kulkulcan
Stranger
Registered: 05/04/16
Posts: 116
Last seen: 6 years, 11 months
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Minimize G2G contams?
#23976294 - 01/02/17 02:05 PM (7 years, 28 days ago) |
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For G2G, would there be less chance for contams by doing 1:3 twice, rather than 1:10 once? Yeah, there would 2 chances for contams to enter, but colonization would be much faster. Maybe it would depend on whether a SAB or FH was used...?
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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Re: Minimize G2G contams? [Re: Kulkulcan]
#23976315 - 01/02/17 02:11 PM (7 years, 28 days ago) |
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Quote:
Kulkulcan said: For G2G, would there be less chance for contams by doing 1:3 twice, rather than 1:10 once? Yeah, there would 2 chances for contams to enter, but colonization would be much faster. Maybe it would depend on whether a SAB or FH was used...?
If you mean transfering 1/10 of a donor jar once vs 1/3 of donor jar twice just wait for the 1/10 to colonize a bit and shake the hell out of it... no need to introduce more contam vectors..
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Kulkulcan
Stranger
Registered: 05/04/16
Posts: 116
Last seen: 6 years, 11 months
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Quote:
mushhiehunter said: If you mean transfering 1/10 of a donor jar once vs 1/3 of donor jar twice...
Yes
Quote:
mushhiehunter said: ...just wait for the 1/10 to colonize a bit and shake the hell out of it...
I read in a tek on this site (can't find it right now) that shaking it again after the initial transfer would actually slow things down, rather than speed them up. But maybe what I read was an old, out of date tek. Have you actually verified for yourself that this works to speed things up?
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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Re: Minimize G2G contams? [Re: Kulkulcan]
#23976368 - 01/02/17 02:31 PM (7 years, 28 days ago) |
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Quote:
Kulkulcan said:
Quote:
mushhiehunter said: If you mean transfering 1/10 of a donor jar once vs 1/3 of donor jar twice...
Yes
Quote:
mushhiehunter said: ...just wait for the 1/10 to colonize a bit and shake the hell out of it...
I read in a tek on this site (can't find it right now) that shaking it again after the initial transfer would actually slow things down, rather than speed them up. But maybe what I read was an old, out of date tek. Have you actually verified for yourself that this works to speed things up?
yes... Quite recently....
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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a great example of a proper and not so proper shake...the left jar was a quick shake the right one was shaken turned shaken again to evenly distribute everything
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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danjaone
Entrepreninja


Registered: 03/19/10
Posts: 12
Loc: 42 miles into your brain
Last seen: 3 years, 3 months
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Re: Minimize G2G contams? [Re: cronicr]
#23976495 - 01/02/17 03:17 PM (7 years, 28 days ago) |
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ive found it only takes longer not shaking them. never really any problem with extra contams or later more easily ruined myc. a lot of people have been saying that not evenly distributed grain shakes will make for more easily contam. opinionns? i have found that to be false...
-------------------- Even chaos has a melody.... act accordningly
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Minimize G2G contams? [Re: danjaone]
#23976506 - 01/02/17 03:21 PM (7 years, 28 days ago) |
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naw not any contam issues just slows shit down
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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