Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinelovelaughlibs
Dopamine Slave
I'm a teapot User Gallery


Registered: 10/14/15
Posts: 1,811
Loc: England Flag
Last seen: 5 years, 11 months
What can I do whilst I wait for my agar?
    #23976240 - 01/02/17 01:46 PM (7 years, 28 days ago)

Hi, a lot of my planned projects (like rye to bulk and some bottles) all revolve around having colonised clean agar to inoculate the substrate with. What can I do in the meantime mycology-wise whilst I wait for my agar to colonise? Are there any projects outside of PF teks I can just use an MS syringe on?


I have some agar that is inoculated right now but due to only 48 hours going by it hasnt germinated yet.


Thanks in advance!


--------------------
Ask and ye shall receive; Seek and ye shall find.


Edited by lovelaughlibs (01/03/17 08:56 AM)


Extras: Filter Print Post Top
OfflineMattisfat
Learning
Male

Registered: 01/20/16
Posts: 570
Loc: Newcastle, England Flag
Last seen: 3 years, 21 days
Re: What can I do whilst I wait for my agar? [Re: lovelaughlibs]
    #23976250 - 01/02/17 01:49 PM (7 years, 28 days ago)

send some to tump so his gf can see what it tastes like


--------------------


Extras: Filter Print Post Top
OfflineGoodkitty
Stranger

Registered: 12/21/15
Posts: 66
Last seen: 6 years, 7 months
Re: What can I do whilst I wait for my agar? [Re: lovelaughlibs]
    #23976285 - 01/02/17 02:02 PM (7 years, 28 days ago)

The proper thing for a young excited mycologist is to order some from the internet then go drive half the day and search the Chinese stores and waste 3 times more than if he waited and get er done sooner.


Extras: Filter Print Post Top
OfflineGoodkitty
Stranger

Registered: 12/21/15
Posts: 66
Last seen: 6 years, 7 months
Re: What can I do whilst I wait for my agar? [Re: Goodkitty]
    #23976286 - 01/02/17 02:03 PM (7 years, 28 days ago)

Du-uh!


Extras: Filter Print Post Top
OfflineGoodkitty
Stranger

Registered: 12/21/15
Posts: 66
Last seen: 6 years, 7 months
Re: What can I do whilst I wait for my agar? [Re: Goodkitty]
    #23976292 - 01/02/17 02:05 PM (7 years, 28 days ago)

Also there are some agar substitutes. https://www.shroomery.org/8514/Agar-substitute for 1 example. you can search for Agar alternatives and try summadatshit


Extras: Filter Print Post Top
Offlinelovelaughlibs
Dopamine Slave
I'm a teapot User Gallery


Registered: 10/14/15
Posts: 1,811
Loc: England Flag
Last seen: 5 years, 11 months
Re: What can I do whilst I wait for my agar? [Re: Goodkitty]
    #23976329 - 01/02/17 02:15 PM (7 years, 28 days ago)

I worded this really badly, sorry. My agar is inoculated but isn't showing growth yet as it's only been 48hours.


--------------------
Ask and ye shall receive; Seek and ye shall find.


Extras: Filter Print Post Top
Offlinelovelaughlibs
Dopamine Slave
I'm a teapot User Gallery


Registered: 10/14/15
Posts: 1,811
Loc: England Flag
Last seen: 5 years, 11 months
Re: What can I do whilst I wait for my agar? [Re: lovelaughlibs]
    #23978178 - 01/03/17 08:56 AM (7 years, 27 days ago)

Bump for inspiration


--------------------
Ask and ye shall receive; Seek and ye shall find.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,281
Loc: where?
Trusted Cultivator
OG Cultivator
Re: What can I do whilst I wait for my agar? [Re: lovelaughlibs] * 1
    #23978184 - 01/03/17 09:00 AM (7 years, 27 days ago)

it can take longer then 48hr for the spores to germinate. sometimes it can take a week.
you need to learn patience.

and you are not supposed to bump your own thread within 24hrs.

PATIENCE YOUNG GRASSHOPPER

go jerk it or something.


Extras: Filter Print Post Top
Offlinewtfcrazymofo
foil hater
Other


Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 11 hours, 18 minutes
Re: What can I do whilst I wait for my agar? [Re: mushboy]
    #23978194 - 01/03/17 09:06 AM (7 years, 27 days ago)

Read up on grain prep li's and sfd's


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




Extras: Filter Print Post Top
Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: What can I do whilst I wait for my agar? [Re: wtfcrazymofo] * 1
    #23978267 - 01/03/17 09:40 AM (7 years, 27 days ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 01:04 PM)


Extras: Filter Print Post Top
InvisiblePirateSwazey
 User Gallery

Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
Trusted Cultivator
Re: What can I do whilst I wait for my agar? [Re: amidogen]
    #23978587 - 01/03/17 11:41 AM (7 years, 27 days ago)

Just save your syringe... One drop is enough to start a culture on agar. It can last you a lifetime if you use it wisely.

If you just started your first plates you will have about 2 weeks of transferring mycelium across plates to ensure you have a clean culture.

In that time I would recommend ordering synthetic filter discs and start prepping your grain masters. If you want to have the best chance at a successful first bulk grow, spawning to the bulk substrate with G1 spawn (inoculated from agar) will put you in a better position than having expanded that mycelium and added a possible vector for contamination.

Since you are so new to agar I would stay away from the liquid teks... There is no need for you to complicate the process right now. Simply dropping wedges to grain has been the standard for a long time for a reason.


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



Extras: Filter Print Post Top
InvisibleKauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,131
Re: What can I do whilst I wait for my agar? [Re: PirateSwazey]
    #23978732 - 01/03/17 12:44 PM (7 years, 27 days ago)

If you want to start a small grow just to have something going, use a bit of your spore syringe on a jar of RGS.  Super easy.  Get a couple of twist n loc PP5 jars ... boil some RGS for a half hour, drain for 10 minutes ... fill the jars about half full and PC them for an hour at 15psi.  Cool, inoculate with a half ML or so and see what happens.  Good chance it will grow out fine and then case and you'll have some fruits in -5-6 weeks    Contamination is a much bigger risk when you do Grain expansions to bulk and fruit in a mono.  For just a single bottle grow with RGS, if you have a clean syringe, you've got a very good chance it will fruit ... no SGFC needed ... grow it right in the bottle ... about the lowest contam risk there is from syringe spores.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


Edited by KauaiOrca (01/03/17 12:49 PM)


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* agar transfers *DELETED* thisone 1,245 3 11/20/03 08:58 PM
by Mycomancer
* How to make agar 2 agar transfer? esp 6,655 7 01/08/04 11:08 AM
by YidakiMan
* Agar plates should i transfer or make lc baddagger 907 4 03/13/18 11:42 AM
by baddagger
* Can you avoid G2G senescence by transferring flesh to Agar? sasquatchisreal 908 11 03/20/18 01:10 PM
by bodhisatta
* Innoculation with agar wedge Visigoth 5,279 6 01/15/23 12:59 PM
by Screwup
* agar questions stara 1,356 6 04/21/04 12:29 PM
by stara
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,285 38 05/11/01 11:08 AM
by MAIA
* Benefits of Agar? celsius 9,382 10 12/01/19 12:36 AM
by wildernessjunkie

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
246 topic views. 19 members, 212 guests and 68 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.007 seconds on 14 queries.