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pheasant327


Registered: 10/04/16
Posts: 133
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First agar attempts. Opinions?
#23974033 - 01/01/17 07:44 PM (7 years, 29 days ago) |
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I'm working with agar for the first time and I'm having a hard time deciding if some of the jars need to be scrapped, or if I'm being paranoid and should keep working with them.
All work was done in my SAB and I have at least two control jars that I used during each session. These were opened and either swiped with a fresh sterile swab, stabbed a few times with the flamed scalpel, or just left open for a few seconds to match the type of transfer to agar that I was doing at the time. None of the control jars have shown any sign of contamination.
One reason that I'm concerned is that all the growth looks fluffier than I'm used to seeing in most photos here. Any thoughts?
#1 - Z-strain clone. Harvested fruit and put tissue to agar 7 days ago. Best looking of my "questionable" jars, but still questionable to me.

#2 - AA+ - Multispore. Swabbed from print 10 days ago.

#3 - PE from spore syringe 12 days ago.

I had a hell of a time trying to get my phone to focus on the surface of the plates and the photos aren't great. I can try to take some better ones tomorrow. Any opinions would be greatly appreciated!
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: First agar attempts. Opinions? [Re: pheasant327]
#23974136 - 01/01/17 08:28 PM (7 years, 29 days ago) |
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What agar reciepe are you working with?
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: First agar attempts. Opinions? [Re: Tookitooki]
#23974179 - 01/01/17 08:48 PM (7 years, 29 days ago) |
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I see no apparent contamination to my untrained eye. Looks like clean tomentose growth.
I think bodhisatta answers it in this thread. One day I'll learn to quote
https://www.shroomery.org/forums/showflat.php/Number/22206022#22206022
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pheasant327

Registered: 10/04/16
Posts: 133
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Re: First agar attempts. Opinions? [Re: Tookitooki]
#23974190 - 01/01/17 08:54 PM (7 years, 29 days ago) |
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Quote:
Tookitooki said: What agar reciepe are you working with?
#1 is on a pre-mixed PDA that I purchased from a sponsor. #2 and #3 are on LMEA (10g LME/10G Agar/500mL water).
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: First agar attempts. Opinions? [Re: pheasant327]
#23974210 - 01/01/17 09:03 PM (7 years, 29 days ago) |
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I know agar reciepe can effect how it grows.
I'd do a transfer for #2 & #3. I believe what I see in #3 is two colonies forming. #2 does look a little funny, see what a transfer lands you.
Are you going from agar to grain master?
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pheasant327

Registered: 10/04/16
Posts: 133
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Re: First agar attempts. Opinions? [Re: Tookitooki]
#23975345 - 01/02/17 08:27 AM (7 years, 28 days ago) |
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Thanks for the input. I guess it can't hurt to make a transfer to see how it grows out on a fresh plate.
Yeah, the plan is go agar to grain and then G2G. I was also thinking about trying Munchauzen's Blenderless LI.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: First agar attempts. Opinions? [Re: pheasant327]
#23975375 - 01/02/17 08:41 AM (7 years, 28 days ago) |
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definitely transfer from 2 and 3. 3 looks the shadiest IMO. 2 is just a bunch of strains, so it looks fluffy like that. 3 is likely the same thing, just looks kinda weirder to me.
that chicken of the woods picture in ur signature though??? fucking A, nice find
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: First agar attempts. Opinions? [Re: blindingleaf]
#23975417 - 01/02/17 09:02 AM (7 years, 28 days ago) |
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Once I can test out my clones, I want to play with that same LI tek. For some reason I fancy LC and LI, I have had good luck with LC, but I've never tried LI yet.
In a few weeks I'll be putting a syringe and print to agar for my first time, so I'm sure ill get a first hand experience of what your going thru right now. I've only ever put clones to agar at this point.
Keep us all posted and keep the pics coming, no matter how many times I see a plate of agar, or jar of spawn, I still get excited.
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pheasant327

Registered: 10/04/16
Posts: 133
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Re: First agar attempts. Opinions? [Re: blindingleaf]
#23975421 - 01/02/17 09:04 AM (7 years, 28 days ago) |
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Quote:
blindingleaf said: definitely transfer from 2 and 3. 3 looks the shadiest IMO. 2 is just a bunch of strains, so it looks fluffy like that. 3 is likely the same thing, just looks kinda weirder to me.
Good to hear. I'll mix up some fresh agar tonight and have a SAB session tomorrow. Where would you transfer from on a clusterfuck of a plate like #2?
Does #1 look okay? Should I just let it grow out a bit more before making a transfer?
Quote:
that chicken of the woods picture in ur signature though??? fucking A, nice find 
Thanks! It was growing up about 8 ft. high on that trunk. All that orange sure was easy to spot! Lucky for me it's in a pretty inaccessible place and the land owner is a friend.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: First agar attempts. Opinions? [Re: pheasant327]
#23975478 - 01/02/17 09:29 AM (7 years, 28 days ago) |
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From 2 I would take from 4-6 o clock.
1 I would wait 3-4 more days. Let it show you what it's all about
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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synthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 10 months
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Re: First agar attempts. Opinions? [Re: blindingleaf]
#23975514 - 01/02/17 09:46 AM (7 years, 28 days ago) |
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thanks for the post op, im waiting on spores with plates ready to jam!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First agar attempts. Opinions? [Re: blindingleaf]
#23975520 - 01/02/17 09:49 AM (7 years, 28 days ago) |
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 Origin MS plates
 one of those MS plates up close after a couple more days
 Both of these are first transfer plates from an original MS dish
Most people would assume the rhizo one is going to be awesome but the tomentose plate could very well outperform the rhizo one.
Don't know till you test.
All you want is clean growth
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pheasant327

Registered: 10/04/16
Posts: 133
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Re: First agar attempts. Opinions? [Re: bodhisatta]
#23975571 - 01/02/17 10:11 AM (7 years, 28 days ago) |
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Quote:
blindingleaf said: From 2 I would take from 4-6 o clock.
1 I would wait 3-4 more days. Let it show you what it's all about
Okay, great. I appreciate the input!
Quote:
bodhisatta said: Both of these are first transfer plates from an original MS dish
Most people would assume the rhizo one is going to be awesome but the tomentose plate could very well outperform the rhizo one.
Don't know till you test.
All you want is clean growth
I know I had that assumption at first. It was one of your posts in another thread that put me at ease about the lack of rhizomorphic growth when they first started taking off last week. Unfortunately, tomentose growth looks similar to pictures that I've seen of some mold varieties on agar (at least to my inexperienced eyes) to make me worry.
Doing this reminds me of how nervous I was when I first started with cakes forever ago. I remember thinking that every sign of growth was a contam during that first run. I didn't miss that paranoid feeling, but the excitement that goes along with learning agar techniques definitely outweighs it!
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: First agar attempts. Opinions? [Re: pheasant327]
#23975592 - 01/02/17 10:19 AM (7 years, 28 days ago) |
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Quote:
pheasant327 said: I didn't miss that paranoid feeling, but the excitement that goes along with learning agar techniques definitely outweighs it!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: First agar attempts. Opinions? [Re: mushboy]
#23975638 - 01/02/17 10:35 AM (7 years, 28 days ago) |
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If you're doing agar at all you're already steps ahead
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synthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 10 months
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Re: First agar attempts. Opinions? [Re: bodhisatta]
#23977252 - 01/02/17 08:33 PM (7 years, 28 days ago) |
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Quote:
bodhisatta said:
Most people would assume the rhizo one is going to be awesome but the tomentose plate could very well outperform the rhizo one.
Don't know till you test.
All you want is clean growth
thanks for this! I thought you wanted only rhizo growth for some reason!
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pheasant327

Registered: 10/04/16
Posts: 133
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Re: First agar attempts. Opinions? [Re: synthetik]
#23978122 - 01/03/17 07:54 AM (7 years, 27 days ago) |
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Thanks all. Got some fresh plates made up and I'm transferring from #2 and #3 today. Will be back with pictures once they grow out.
Also, what do you guys do with multispore plates that have grown to the edges of the plates? Keep them to work from/see if they pin or clear them out?
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Intelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
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Re: First agar attempts. Opinions? [Re: pheasant327]
#23978152 - 01/03/17 08:26 AM (7 years, 27 days ago) |
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theres no harm in keeping the plates
I'd say they are all good, 2 does look a bit suspicious but I think your transfer should show clean growth
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
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Re: First agar attempts. Opinions? [Re: pheasant327]
#23978357 - 01/03/17 10:17 AM (7 years, 27 days ago) |
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Keep them around until they pin. If they do then take a clone. 1 transfer away from the clone is awesome stuff to knock up anything.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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pheasant327

Registered: 10/04/16
Posts: 133
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Re: First agar attempts. Opinions? [Re: wtfcrazymofo]
#23996576 - 01/09/17 08:51 PM (7 years, 21 days ago) |
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Hokay, so, it's been 8 days since I originally posted and here's where #1 is today (16 days since putting tissue to agar).
It's looking pretty good to me, but I wouldn't mind some experienced eyes. Does it still look good to you guys?

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