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OfflineCubeSteakPoo
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BRF Contam Issues, Please Help
    #23971416 - 12/31/16 05:29 PM (7 years, 30 days ago)

Hey Guys,

I am having an issue with my BRF Jars after innoculating. Last year when I did this I had a 100% success rate. Out of 9 jars I inoculated, I had 0 contaminants and only 1 jar didn't colonize. This year I am following the same steps as before but I am running into a slight foot odor in my jars just 3 days after inoculation. Here is a run down of what i did

1. After preparing the substrate with a 2:1:1 ratio. I placed the substrate in the jars nice and fluffy, added the dry verm layer starting about a half inch from the top. After that i placed a peice of aluminum foil on the jar, put the jar lid on top (with 4 holes punched through) and then the screw lid on to ensure a a complete seal.

2. I pressure cooked for 1.5 hours at 12psi, let cool thoroughly for 24 hours then proceeded to turn my bathroom into a sterile ass environment, hanging plastic to seperate the work area from the toilet and shower, wearing a shower cap, respirator, gloves, and a rain poncho. Aersosolled, alcohol swabbed myself multiple times with 70% isopropyl. flame sterilized needle after each jar. the whole nine yards (same exact procedure i did last year with complete success)

once inoculated, i placed the jars on my , what i call an "open incubator"

it consists of having a seed mat underneath a metal rack (mat and rack seperated by about a half inch"

the seed mat has a thermometer on it which i placed in the open air about 3 inches from the heat mat and set it to 74 degrees.

For some reason , these jars are smelling like feet, and this is the second time i have done this and they are smelling like feet again.

based on what i have told you, does this seem like my spore syringes are garbage? how can all my jars smell contaminated, when last time i had 0 contamination? all my steps have remained consistent with last time.

I only used about 1 cc per jar,

is it possible that theses jars could smell a little funky from the water itself?

any thoughts would be appreciated


Edited by CubeSteakPoo (12/31/16 05:56 PM)


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InvisibleMad Season
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Re: BRF Contam Issues, Please Help [Re: CubeSteakPoo]
    #23972610 - 01/01/17 09:49 AM (7 years, 29 days ago)

Smells aren't good at all. Especially feet smell. That is most closely related to bacteria. Sounds like a faulty syringe mate if literally everything gets it.


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OfflineJimharbaugh04
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Re: BRF Contam Issues, Please Help [Re: Mad Season]
    #23972692 - 01/01/17 10:38 AM (7 years, 29 days ago)

Contamination?

Great fruiting conditions and good fae. No bad smells.



Edited by Jimharbaugh04 (01/01/17 10:40 AM)


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OfflineCubeSteakPoo
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Re: BRF Contam Issues, Please Help [Re: Mad Season]
    #23972714 - 01/01/17 10:55 AM (7 years, 29 days ago)

thanks for the reply mad,

I just want to add a couple things here.

So Im wondering if its possible that I mixed up my BRF substrate incorrectly, like if its possible that just a slight measurement off ,and i could of imbalanaced the moisture content  enough to invite bacterial infection.

But what i dont understand, is even if the moisture content was imbalance, and there was more moisture in the jar then there should be, shouldnt my jars still not be contaminated? since i pressure cooked them at 12psi for 90 minutes.

I happened to have 1cc left of this spore syringe, and i injected in a jar, that i mixxed the 2:1:1 BRF mix in. This time i made sure my measurement was exact exact exact. And just for shits and gigs i barely followed any sterile procedure, just alcoholed my gloves arms, surface and jar lid, syringe, etc, and inoculated. But with this jar, I just set it on top of the fridge in norm room temperature, so see if my seed mat could be the problem.So, I'll use this as a Variable jar to see what happends.

I'm wondering if the seed mat reading "72" degrees is actually bringing the internal temp of the jars a lot higher. and possibly being a medium for contaminants.

Also another thing to ad, when i made this extra "Variable jar" i didnt use the actual lid that came with the jar (that i added holes on for innoc) instead, i put a peice of aluminum foil over jar, and put the screwlid over top to make a seal. When i pulled it out of the pressure cooker, I could hear a tiny faint whistling sound coming from the jar. indicating to me that somewhere on that lid, the foil didnt make a seal and there is an air gap. I'm wondering if this happened to ALL my other jars and it went unnoticed. And possibly this tiny air gap, sucking pressure into the jar, was vaccuum sucking contaminants into the jar, and therefore dooming it to contamination.



Also, I got this Sterile syringe from Mirco-supply, i know im not suppose to use non vendor approved names, but this is for educational purposes.

Last year i got from them and i had no problem, spores actually seemed to be from robust species. this year, not one syringe worked, so im wondering if this company just isnt reliable for consistent spores or not.

Let me know if anyone has anything to add, i know i may ramble on, but im just trying to get as much info about my situation as i can out there, and hope for some good feedback.

Thanks Guys!


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OfflineCubeSteakPoo
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Re: BRF Contam Issues, Please Help [Re: CubeSteakPoo]
    #23972717 - 01/01/17 11:01 AM (7 years, 29 days ago)

i had a problem like this last year my first time, I actually have a thread if you search my threads.


Although your issue here seems to have a slight discoloration on your cakes, which mine did not have so it could be different.

Also it appears as thought your terrarium has 0 holes in it, you need many holes in that chamber.

It apprears, correct me if im wrong, that you have attempted to build the auto-terrarium. If so the "positive release pressure" valve or whatever its called , does not provide sufficient FAE, you need to put holes all around that thing,

that aerial mycelium is  from too high humidity, which in turn is because the lack of holes  giving it not enough FAE

Someone correct me if im wrong but i think im right,


Go search my threads from last year, you should see a post i made that is almost identical to ths


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OfflineJimharbaugh04
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Re: BRF Contam Issues, Please Help [Re: CubeSteakPoo]
    #23972748 - 01/01/17 11:21 AM (7 years, 29 days ago)

Will do I'll do some searching thank you again.

I agree on the holes part. I have a series of small holes (20) just above the hydrogen pebbles. I was trying to maintain a positive pressure in the fc. I have mister in a bucket (sealed) with two fishtank pumps.keeping it foggy. My theory is to maintain high RH without adding heat. I just open it and fan it often. Too much fog potentially the problem?


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OfflineCubeSteakPoo
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Re: BRF Contam Issues, Please Help [Re: Jimharbaugh04]
    #23972825 - 01/01/17 11:55 AM (7 years, 29 days ago)

Yes you are making the same exact mistake i did.

the design of your terrarium works good, as long as you have the holes on ALL sides of the terrarium, even the bottom.

follow one of the SGFC teks, as far as the holes go. I think its like a quarter inch hole every 2 inches on all sides, something like that.

but the apparatus you built with the air pump and humidifer works great as a substitute for misting and fanning ( i added a small computer fan on the opposite side of where the humidity gets pumped in to give it a good circulation, which mimics the "fanning" and humidifier mimics the misting part.

I also have that apparatus on a timer, so the Fan, Mister, and air pump will run for 25 minutes every 3 hours, and with only 2 inches of pearlite ( or those beads your using should be sufficcient, although ive never used them) it maintains the humidity at 95% + with plenty of air flow. that is what your doing wrong, the positive pressure thing does not work, you have to have those holes in there,

the good news is that your cakes can be saved. once i drilled all the holes in my terrarium, the cakes appeared to dry up, which made me think they were doomed. But a week after i fixed it, i had the first signs of pinning


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OfflineCubeSteakPoo
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Re: BRF Contam Issues, Please Help [Re: Jimharbaugh04]
    #23972832 - 01/01/17 11:57 AM (7 years, 29 days ago)

here is the thread i made from a year ago if you are interested,

https://www.shroomery.org/forums/showflat.php/Number/23160372


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OfflineJimharbaugh04
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Re: BRF Contam Issues, Please Help [Re: CubeSteakPoo]
    #23972951 - 01/01/17 12:50 PM (7 years, 29 days ago)

You're right same exact problem. Thanks for the support.


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OfflineCubeSteakPoo
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Re: BRF Contam Issues, Please Help [Re: Jimharbaugh04]
    #23972989 - 01/01/17 01:05 PM (7 years, 29 days ago)

if you dont mind me asking, what vendor did you get your spore syringes from?


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OfflineJimharbaugh04
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Re: BRF Contam Issues, Please Help [Re: CubeSteakPoo]
    #23973068 - 01/01/17 01:35 PM (7 years, 29 days ago)

I bought it a year ago. From a vendor that was recommended through this site. Why do you ask?


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