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InvisibleLostsmiles
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Bacteria?
    #23970693 - 12/31/16 12:53 PM (7 years, 30 days ago)

These are the first transfers from MS, done a day or two ago.
There's a build up of this whitish substance on the dishes, it doesn't really stick to it, when I move the plate it falls off with the excess water.
This is my first time using malt agar and Petri dishes. I seem to be having a bit of a problem with condensation in the dish, probably about a two ML worth. I was thinking maybe this water is breaking down the agar and sugar and causing floaters? Or is this bacteria or something like that? Any suggestions on cutting down on condensation?



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InvisibleMycolorado
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Re: Bacteria? [Re: Lostsmiles]
    #23970708 - 12/31/16 01:00 PM (7 years, 30 days ago)

Yep...those are bacteria plates...chuck them.  What's your recipe and method of prep?  Any pics of the ms plates?


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Invisiblepablokabute
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Re: Bacteria? [Re: Mycolorado]
    #23970802 - 12/31/16 01:36 PM (7 years, 30 days ago)

it should have a nasty smell too. try doing a whif...


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InvisibleLostsmiles
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Re: Bacteria? [Re: Mycolorado]
    #23970805 - 12/31/16 01:37 PM (7 years, 30 days ago)

Shit:facepalm3:
It's a premix from out grow, MEA.
I just simmer it for a min, pour too plates, and sterilize at 10 psi for 15min.
I don't have any pics of the MS jar. There's no way to clean it? Or just really hard?
Anything specific that causes bacterial growth, other than my sterile technique, obviously.


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InvisiblemushboyMDiscord
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Re: Bacteria? [Re: Lostsmiles]
    #23970827 - 12/31/16 01:43 PM (7 years, 30 days ago)

10psi for 15min?

i do 15psi for 40 :shrug:


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InvisibleLostsmiles
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Re: Bacteria? [Re: pablokabute]
    #23970847 - 12/31/16 01:49 PM (7 years, 30 days ago)

Quote:

mushboy said:
10psi for 15min?

i do 15psi for 40 :shrug:



That's what I though, but the plates started melting.
Quote:

pablokabute said:
it should have a nasty smell too. try doing a whif...



It definitely had the funky smell


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Invisiblepablokabute
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Re: Bacteria? [Re: Lostsmiles]
    #23970860 - 12/31/16 01:52 PM (7 years, 30 days ago)

melting? is this the disposable plastic petri?


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Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


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InvisibleLostsmiles
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Re: Bacteria? [Re: pablokabute]
    #23970881 - 12/31/16 01:58 PM (7 years, 30 days ago)

Quote:

pablokabute said:
melting? is this the disposable plastic petri?



No there glass.. hahah
Yes plastic, I had them giving to me. I was using mason jars before so I though these would be much better. Guess I'm just waistin time with these then huh?
Glass it is


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Invisiblepablokabute
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Re: Bacteria? [Re: Lostsmiles]
    #23970897 - 12/31/16 02:03 PM (7 years, 30 days ago)

Quote:

Lostsmiles said:
Quote:

pablokabute said:
melting? is this the disposable plastic petri?



No there glass.. hahah
Yes plastic, I had them giving to me. I was using mason jars before so I though these would be much better. Guess I'm just waistin time with these then huh?
Glass it is





you should take a look at 'pasty easy agar tek'... just search for it in the forums.

its a no pour agar technique that uses polypropylene (PP) plastic containers for agar work. It might be your thing.

if you havent seen it yet try reading it up.


Edited by pablokabute (12/31/16 02:08 PM)


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OfflineKenetic
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Re: Bacteria? [Re: mushboy]
    #23971522 - 12/31/16 06:21 PM (7 years, 30 days ago)

Quote:

mushboy said:
10psi for 15min?

i do 15psi for 40 :shrug:




I do 30 min at 10 psi

And op you can't sterilize ps dishes.  They have to be pp or glass.  The idea is to sterilize the media in a bottle and then pour into the dishes later


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InvisibleLostsmiles
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Re: Bacteria? [Re: Kenetic]
    #23973235 - 01/01/17 02:47 PM (7 years, 29 days ago)

Hm, well I forgot that I sterilized 5 dishes but only used 3. The other two seem to be fine.. They have been in the same environment for the same amount of time as the other plates.
I guess it's just something with the syringes or glove box that I'm using. Will have to scrutinize my techniques, I'm a little rusty.


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OfflineKenetic
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Re: Bacteria? [Re: Lostsmiles]
    #23973412 - 01/01/17 03:55 PM (7 years, 29 days ago)

why don't you just pour it yourself instead of dealing with all that condensation?


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleLostsmiles
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Re: Bacteria? [Re: Kenetic]
    #23973494 - 01/01/17 04:39 PM (7 years, 29 days ago)

Quote:

kenetic said:
why don't you just pour it yourself instead of dealing with all that condensation?



What do you mean pour it myself, I'm just getting into agar really so I'm unfamiliar with the different techniques.
When you say sterilize then pour it confuses me cause it solidifies so fast that I have no time to get it into the glove box and sterilized before it hardens


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