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Lostsmiles
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Registered: 12/10/13
Posts: 705
Loc: Dontworryboutiiiit
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Bacteria?
#23970693 - 12/31/16 12:53 PM (7 years, 30 days ago) |
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These are the first transfers from MS, done a day or two ago. There's a build up of this whitish substance on the dishes, it doesn't really stick to it, when I move the plate it falls off with the excess water. This is my first time using malt agar and Petri dishes. I seem to be having a bit of a problem with condensation in the dish, probably about a two ML worth. I was thinking maybe this water is breaking down the agar and sugar and causing floaters? Or is this bacteria or something like that? Any suggestions on cutting down on condensation?

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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Yep...those are bacteria plates...chuck them. What's your recipe and method of prep? Any pics of the ms plates?
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,163
Loc: rural ghetto
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it should have a nasty smell too. try doing a whif...
--------------------
Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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Lostsmiles
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Registered: 12/10/13
Posts: 705
Loc: Dontworryboutiiiit
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Shit It's a premix from out grow, MEA. I just simmer it for a min, pour too plates, and sterilize at 10 psi for 15min. I don't have any pics of the MS jar. There's no way to clean it? Or just really hard? Anything specific that causes bacterial growth, other than my sterile technique, obviously.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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10psi for 15min?
i do 15psi for 40
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Lostsmiles
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Registered: 12/10/13
Posts: 705
Loc: Dontworryboutiiiit
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Quote:
mushboy said: 10psi for 15min?
i do 15psi for 40 
That's what I though, but the plates started melting.
Quote:
pablokabute said: it should have a nasty smell too. try doing a whif...
It definitely had the funky smell
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,163
Loc: rural ghetto
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melting? is this the disposable plastic petri?
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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Lostsmiles
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Registered: 12/10/13
Posts: 705
Loc: Dontworryboutiiiit
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Quote:
pablokabute said: melting? is this the disposable plastic petri?
No there glass.. hahah Yes plastic, I had them giving to me. I was using mason jars before so I though these would be much better. Guess I'm just waistin time with these then huh? Glass it is
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,163
Loc: rural ghetto
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Quote:
Lostsmiles said:
Quote:
pablokabute said: melting? is this the disposable plastic petri?
No there glass.. hahah Yes plastic, I had them giving to me. I was using mason jars before so I though these would be much better. Guess I'm just waistin time with these then huh? Glass it is
you should take a look at 'pasty easy agar tek'... just search for it in the forums.
its a no pour agar technique that uses polypropylene (PP) plastic containers for agar work. It might be your thing.
if you havent seen it yet try reading it up.
Edited by pablokabute (12/31/16 02:08 PM)
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Bacteria? [Re: mushboy]
#23971522 - 12/31/16 06:21 PM (7 years, 30 days ago) |
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Quote:
mushboy said: 10psi for 15min?
i do 15psi for 40 
I do 30 min at 10 psi
And op you can't sterilize ps dishes. They have to be pp or glass. The idea is to sterilize the media in a bottle and then pour into the dishes later
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Lostsmiles
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Registered: 12/10/13
Posts: 705
Loc: Dontworryboutiiiit
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Re: Bacteria? [Re: Kenetic]
#23973235 - 01/01/17 02:47 PM (7 years, 29 days ago) |
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Hm, well I forgot that I sterilized 5 dishes but only used 3. The other two seem to be fine.. They have been in the same environment for the same amount of time as the other plates. I guess it's just something with the syringes or glove box that I'm using. Will have to scrutinize my techniques, I'm a little rusty.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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why don't you just pour it yourself instead of dealing with all that condensation?
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Lostsmiles
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Registered: 12/10/13
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Loc: Dontworryboutiiiit
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Re: Bacteria? [Re: Kenetic]
#23973494 - 01/01/17 04:39 PM (7 years, 29 days ago) |
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Quote:
kenetic said: why don't you just pour it yourself instead of dealing with all that condensation?
What do you mean pour it myself, I'm just getting into agar really so I'm unfamiliar with the different techniques. When you say sterilize then pour it confuses me cause it solidifies so fast that I have no time to get it into the glove box and sterilized before it hardens
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