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divineconspiracy
Noob


Registered: 08/10/16
Posts: 18
Last seen: 7 years, 1 month
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Questions about my next steps/plans with rye berries. Filters?
#23968731 - 12/30/16 05:37 PM (7 years, 1 month ago) |
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I am going to try growing on rye soon, but there doesn't seem to be as much info out there as there is for the PF tek so I want to talk about my plans and ask y'all some things.
From a spore print from my PF tek, I have what I believe is 7 clean fully colonized PDA agar plates which were started in a glove box (1 became moldy and 2 which were inoculated from a separate syringe I ordered, rapidly became bacterial) The 7 that remain are totally covered with translucent white rhizo growth, now some areas are becoming thicker white fuzz.
So I've read that you can use 1/4 of a plate of mycelium to add to a rye jar. Is that right?
I have 25 lbs of rye and 10 quart jars... probably will need more jars huh.
What else will I need?
I saw on the free RR video that you need to make a filter for the top of the jar. Should I try to save up the 8.99 for the videos?
Do I really need to make the filter for the top of the jar? I thought you could use unadulterated jar lids. Honestly I would rather slow colonization than add something which could increase exposure to contams... since my home is a bit moldy. But I would not want to skip the filter if it is impossible for the jar to get 100% colonized without it.
Is the filter absolutely necessary? What type of filter? Tyvek? How do I know what I'm getting is really tyvek? So you pc that material?
Edited by divineconspiracy (12/30/16 05:44 PM)
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,310
Last seen: 22 days, 1 minute
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Re: Questions about my next steps/plans with rye berries. Filters? [Re: divineconspiracy]
#23968786 - 12/30/16 06:06 PM (7 years, 1 month ago) |
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I would transfer them again and experiment with smaller rye batches as you get closer to an isolate. The plates that didnlt get contamed are actually contamed. I would transfer a clean section off 10-20 times then watch those closely.
then Eventually get the best possible semi isolate or hard isolate for best fruiting.
Edited by Groo (12/30/16 06:10 PM)
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,310
Last seen: 22 days, 1 minute
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Re: Questions about my next steps/plans with rye berries. Filters? [Re: Groo]
#23968796 - 12/30/16 06:11 PM (7 years, 1 month ago) |
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Don't get too hung up an anything at all. Glass jars with a hole punched through and some filter material stuffed through will work fine. You have what you need.
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divineconspiracy
Noob


Registered: 08/10/16
Posts: 18
Last seen: 7 years, 1 month
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Re: Questions about my next steps/plans with rye berries. Filters? [Re: Groo]
#23968809 - 12/30/16 06:17 PM (7 years, 1 month ago) |
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I'm not planning on transferring from plate to plate. I don't have any more agar or money to order any right now and the shrooms were great last time from the PF tek. I'm not trying to isolate the fastest growing mycelium right now. I just want to start some jars. I will save a plate for the future but I'm not ready to start transferring from plate to plate right now. I'm just preparing to start some rye jars.
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divineconspiracy
Noob


Registered: 08/10/16
Posts: 18
Last seen: 7 years, 1 month
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Re: Questions about my next steps/plans with rye berries. Filters? [Re: divineconspiracy]
#23968817 - 12/30/16 06:18 PM (7 years, 1 month ago) |
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How do you know they are contaminated?
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,310
Last seen: 22 days, 1 minute
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Re: Questions about my next steps/plans with rye berries. Filters? [Re: divineconspiracy]
#23968834 - 12/30/16 06:23 PM (7 years, 1 month ago) |
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half your others are con tamed, Just cuz you mycellium won the battle on the middle of the petri the shit may seem contained it probably isn't and is growing in the middle and among-st your myc. you have to transfer and isolate more 1 or 2 more times till you can be SURE you are even clean. Sounds like you need a lil agar agar I got you if you send pm me your addy Ill send you 15 petri dishes and agar and all the shit u need. If you start jars now start from the best looking mycellium edges and as you move in closer to the inside expect contams.
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