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OfflineDr_Sativa
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Re: Dr_Sativa's isolated strain shoot out! [Re: Dr_Sativa]
    #23994487 - 01/09/17 06:46 AM (7 years, 21 days ago)

The stuff on the bottom has been colonized, so i think its safe to say its not bacteria.


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OfflineGuess
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Re: Dr_Sativa's isolated strain shoot out! [Re: Dr_Sativa]
    #23999770 - 01/10/17 10:37 PM (7 years, 20 days ago)

Good stuff, the way I prep my grains it happens to me sometimes with the last couple jars. It's never been an issue :shrug:
I clean the grain, then pour boiling water over it and let it soak for 6 - 10 hours (stirring at least twice). Easy and works well.


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OfflineDr_Sativa
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Re: Dr_Sativa's isolated strain shoot out! [Re: Guess]
    #24020749 - 01/18/17 05:31 PM (7 years, 12 days ago)

Jars are primed and ready to go

lost one along the way.. but its fighting back! still dumping (type 3)


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OfflineThedillestpickle
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Re: Dr_Sativa's isolated strain shoot out! [Re: Dr_Sativa]
    #24020854 - 01/18/17 06:10 PM (7 years, 12 days ago)

weird, for me any standing moisture in a jar turns the uncolonized grain it touches bacterial before it can ever get a chance to colonize.  So in my experience wet grain=bacteria 

I always shake my jars a few times after removing from PC to distribute moisture back into the drier grains.

Have you grown out any of these strains before?

Hopefully you get a clear winner!


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OfflineDr_Sativa
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Re: Dr_Sativa's isolated strain shoot out! [Re: Thedillestpickle]
    #24021556 - 01/18/17 10:16 PM (7 years, 12 days ago)

I would have thought that sterilization would kill off everything?

I'm guessing it originated from work in the SAB.

Upon closer inspection it looks to me like green mold. What do you think?




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Offlinetump
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Re: Dr_Sativa's isolated strain shoot out! [Re: Dr_Sativa]
    #24021665 - 01/18/17 11:11 PM (7 years, 12 days ago)

Nope looks like black mold


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OfflineAdas
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Re: Dr_Sativa's isolated strain shoot out! [Re: Dr_Sativa]
    #24021809 - 01/19/17 01:16 AM (7 years, 12 days ago)

Quote:

Dr_Sativa said:
I would have thought that sterilization would kill off everything?





Depends on your technique. Some bacterial spores are very resistant. Some might even survive pressure cooking. Fractional sterilization is the way to go. Works even without the PC.


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OfflineThedillestpickle
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Re: Dr_Sativa's isolated strain shoot out! [Re: Adas]
    #24022003 - 01/19/17 05:31 AM (7 years, 11 days ago)

I would say it was introduced in the SAB.  Bacteria can survive PC but as far as I know molds cannot.


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OfflineCagney
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Re: Dr_Sativa's isolated strain shoot out! [Re: Thedillestpickle]
    #24022029 - 01/19/17 05:48 AM (7 years, 11 days ago)

:popcorn:


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OfflineGuess
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Re: Dr_Sativa's isolated strain shoot out! [Re: Cagney]
    #24022834 - 01/19/17 12:33 PM (7 years, 11 days ago)

1 lost jar is nothing to sweat over, and I agree with tump, black mold. It most likely came from SAB work, even if you were perfect, black mold is common in closets, bathrooms, and kitchens.  But mainly crawl spaces that are lacking proper drainage.

I bet you did SAB work in a closet that has a wall adjacent bathroom or the kitchen?

I worked for a restoration company for a while, we specialized in killing black, yellow, and white molds. Shit was gross. The bad cases of black mold were always caused by a unnoticed leak or lack of drainage and dehumidifiers in crawl spaces. Insulation in crawl spaces would be covered in it, we would tear all that out and kill what was growing on the wood by spraying and rubbing Shockwave into the wood with rags (hopefully killing spores in the process). Then the team of painters would come in and do a sealant, then we would dig drainage ditches and replace sub pumps. I learned a lot about mold through that job. Gross as hell though, I do not recommend.


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OfflineDr_Sativa
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Re: Dr_Sativa's isolated strain shoot out! [Re: Guess]
    #24022955 - 01/19/17 01:37 PM (7 years, 11 days ago)

There is a vent right above where I used the SAB. Might have been a pressure difference push out some spores.


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OfflineGuess
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Re: Dr_Sativa's isolated strain shoot out! [Re: Dr_Sativa]
    #24034726 - 01/23/17 10:34 PM (7 years, 7 days ago)

I like that observation, definitely could be a factor :thumbup: Your contam rate wasn't nearly as bad as mine is right now.. I have to keep the seal on my PC with clamps and it takes forever to build pressure if it even does so this round I've been crossing my fingers. Lost one tub of what I thought to be clean spawn to bacteria, lost another to trich (I think that was me on pasteurization), and a bunch of jars didn't even do anything because of the bacteria present  :facepalm3:
I'm so close to that new PC....

Still have 3 tubs and a mini plus 6 clean looking jars that I'm going to spawn into more minis. Only one is fruiting right now though and the others should have pins already :tongue:
I'm pretty sure the one that is has a wee bit of bacteria too.

Awaiting an update! Something has to be brewing, Lets see the tubs!


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