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OfflineMushieman12
Stranger
Registered: 09/10/16
Posts: 87
Last seen: 3 years, 11 months
black stuff in jars
    #23967273 - 12/30/16 03:37 AM (7 years, 1 month ago)

Hi, can anyone tell me what this black stuff is?
Should I toss em?
How could I prevent this from happening again?

Thank you







These are 3 different jars inoc at the same time with the same syringe.


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InvisibleInvader Zim
Peace Love & Hippyness
Male


Registered: 05/14/14
Posts: 387
Loc: Maritimes Flag
Re: black stuff in jars [Re: Mushieman12]
    #23967368 - 12/30/16 06:02 AM (7 years, 1 month ago)

The black stuff is mold, just toss them. To prevent it, study/practice sterile technique. Where did you do the innoculations? Did you flame sterile the syringe tip, alcohol sanitize everything else in proximity to your work area? It should go without saying, but you should make a still air box if you haven't yet.


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
Re: black stuff in jars [Re: Invader Zim]
    #23968485 - 12/30/16 03:42 PM (7 years, 1 month ago)

:whathesaid:

Those are contaminated and will not do any good, sorry.


--------------------
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Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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OfflineMushieman12
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Registered: 09/10/16
Posts: 87
Last seen: 3 years, 11 months
Re: black stuff in jars [Re: Invader Zim]
    #23969465 - 12/30/16 10:31 PM (7 years, 30 days ago)

Yeh to all the above. Sterile room with still box, flame sterilised needle ect.
Maybe the syringe is contaminated. Ill give it another crack. Thanks a lot guys. Have a nice new yr


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InvisibleInvader Zim
Peace Love & Hippyness
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Registered: 05/14/14
Posts: 387
Loc: Maritimes Flag
Re: black stuff in jars [Re: Mushieman12]
    #23970239 - 12/31/16 09:23 AM (7 years, 30 days ago)

I think you're probably on the right track thinking it was the dirty syringe... I think it's allowed to name vendors, right? I've always had great results veiwing spores from Sporeworks.

Better luck next time and happy new year's


Edited by Invader Zim (12/31/16 09:37 AM)


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InvisibleInvader Zim
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Registered: 05/14/14
Posts: 387
Loc: Maritimes Flag
Re: black stuff in jars [Re: Invader Zim]
    #23970247 - 12/31/16 09:27 AM (7 years, 30 days ago)

Oh yeah, do agar! You won't regret it. I share this a lot, check it out; https://www.shroomery.org/forums/showflat.php/Number/18430998


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
Re: black stuff in jars [Re: Invader Zim]
    #23970265 - 12/31/16 09:34 AM (7 years, 30 days ago)

Quote:


Invader Zim said:

I think it's allowed to name vendors, right?




Nope.


Quote:


3.  Spores are sold for microscopy use only so mycologists can ID mushrooms. If you're going to misuse them to grow a controlled substance, keep it to yourself.  Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning.  Do not plug non sponsors.




Unless you're talking about their use in microscopy, you shouldn't name vendors.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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InvisibleInvader Zim
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Male


Registered: 05/14/14
Posts: 387
Loc: Maritimes Flag
Re: black stuff in jars [Re: Tira]
    #23970271 - 12/31/16 09:38 AM (7 years, 30 days ago)

Good save, thanks. Better? Haha.

I just noticed a species was never mentioned.


Edited by Invader Zim (12/31/16 09:40 AM)


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
Re: black stuff in jars [Re: Invader Zim]
    #23970387 - 12/31/16 10:29 AM (7 years, 30 days ago)

Quote:

Invader Zim said:
I've always had great results viewing spores from Sporeworks.





Yeah me too, could watch them for hours lol


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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OfflineMushieman12
Stranger
Registered: 09/10/16
Posts: 87
Last seen: 3 years, 11 months
Re: black stuff in jars [Re: Tira]
    #23977884 - 01/03/17 02:24 AM (7 years, 27 days ago)

Thanks guys. Ill check out the agar, don't know much about it yet, don't know what the benefit is yet either.
The jars where Suposed to be golden teachers


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OfflineMushieman12
Stranger
Registered: 09/10/16
Posts: 87
Last seen: 3 years, 11 months
Re: black stuff in jars [Re: Tira]
    #23977906 - 01/03/17 02:38 AM (7 years, 27 days ago)

Also what if the black stuff is gone. Is it possible the mycelium destroyed it?


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InvisibleInvader Zim
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Registered: 05/14/14
Posts: 387
Loc: Maritimes Flag
Re: black stuff in jars [Re: Mushieman12]
    #23978028 - 01/03/17 06:02 AM (7 years, 27 days ago)

If you follow the link I posted you will learn a lot about agar and how to use it. It is very important to learn, especially to clean up dirty syringes like you may have.

If the mycelium grows over the black mold I'd say you could try to fruit, but you'd be likely to cross-contaminant any other project in proximity to the moldy cake. Maybe place it outside if in a warm environment, otherwise it would be better to just try again. Take the time and learn agar for better success.


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OfflineTira
I'm a teapot


Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
Re: black stuff in jars [Re: Mushieman12]
    #23978069 - 01/03/17 06:56 AM (7 years, 27 days ago)

Quote:

Mushieman12 said:
Also what if the black stuff is gone. Is it possible the mycelium destroyed it?




I'm sorry, no.  Especially if it is contaminated at an early stage such as this one.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


Extras: Filter Print Post Top
OfflineMushieman12
Stranger
Registered: 09/10/16
Posts: 87
Last seen: 3 years, 11 months
Re: black stuff in jars [Re: Tira]
    #23978673 - 01/03/17 12:15 PM (7 years, 27 days ago)

I will look into agar. Thanks again.
Be safe


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