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naykid
computers.suck
Registered: 05/06/14
Posts: 167
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How does this sound for a simple tek?
#23965859 - 12/29/16 03:30 PM (7 years, 2 months ago) |
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soak rye berries over night, drain and refill water, simmer rye berries in pot until boil on low heat for 10 mins, then drain. fill 16 oz wide mouthed jar until 3/4 - 4/5 full, place tyvek over top of jar then metal cap with small holes punched for inoculation ports, then screw on lid. place jars in pressure cooker for correct time and temp/psi after completely cooled down then inoculate with syringe and place in incubator until colonization is complete.
Is there any improvements I can make?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: How does this sound for a simple tek? [Re: naykid]
#23965915 - 12/29/16 03:45 PM (7 years, 2 months ago) |
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Agar
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: How does this sound for a simple tek? [Re: van hatton]
#23965951 - 12/29/16 03:58 PM (7 years, 2 months ago) |
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I don't see how this is an improvement over existing grain prep teks.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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enlightenment
alchemist



Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
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Re: How does this sound for a simple tek? [Re: van hatton]
#23965955 - 12/29/16 03:59 PM (7 years, 2 months ago) |
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Quote:
soak rye berries over night, drain and refill water, simmer rye berries in pot until boil on low heat for 10 mins, then drain. fill 16 oz wide mouthed jar until 3/4 - 4/5 full, place tyvek over top of jar then metal cap with small holes punched for inoculation ports, then screw on lid. place jars in pressure cooker for correct time (at least 90 minutes) and temp/psi (15 PSI) after completely cooled down then inoculate with syringe and place in incubator until colonization is complete. Usually there is no need for an incubator. What is the temperature in your room?
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naykid
computers.suck
Registered: 05/06/14
Posts: 167
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Re: How does this sound for a simple tek? [Re: van hatton]
#23966134 - 12/29/16 05:27 PM (7 years, 2 months ago) |
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Quote:
van hatton said: Agar 
why agar personally? I would liketo experiment with it one day, but for sake of cost I don't find it worth it right now.
if a spore syringe is made from 1 cap wouldn't the spores all be very similar already so that a test to see variation wouldn't yield different phenotypes as would be the purpose as i see of using agar plates? or is there a worthwhile reason I'm over looking?
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
Posts: 1,811
Loc: England
Last seen: 6 years, 30 days
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Re: How does this sound for a simple tek? [Re: naykid]
#23966166 - 12/29/16 05:43 PM (7 years, 2 months ago) |
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Quote:
naykid said:
Quote:
van hatton said: Agar 
why agar personally? I would liketo experiment with it one day, but for sake of cost I don't find it worth it right now.
if a spore syringe is made from 1 cap wouldn't the spores all be very similar already so that a test to see variation wouldn't yield different phenotypes as would be the purpose as i see of using agar plates? or is there a worthwhile reason I'm over looking?
Need to get rid of the inevitable bacteria/rival species that syringes carry and that agar makes visible
-------------------- Ask and ye shall receive; Seek and ye shall find.
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Hekate
Probiotic

Registered: 12/23/12
Posts: 68
Last seen: 6 years, 11 months
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Re: How does this sound for a simple tek? [Re: naykid]
#23966167 - 12/29/16 05:44 PM (7 years, 2 months ago) |
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No need to drain that water. If anything stir your grains up and pour off dirty water a few times. Add 1/4 tsp gypsum per cup of dry grain into final soak water Let sit for a day, short boil, drain, load. By incubation I hope you mean under 80F.
Consider upgrading to some rtv high temp gasket maker for self healing points and synthetic filter discs for gas exchange. Shit is cheap af and significantly better.
You can do agar cheap too. Just need to read others methods. The sooner you get into that, the sooner you level up.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: How does this sound for a simple tek? [Re: enlightenment]
#23966169 - 12/29/16 05:45 PM (7 years, 2 months ago) |
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Quote:
enlightenment said:

Quote:
soak rye berries over night, drain and refill water, simmer rye berries in pot until boil on low heat for 10 mins, then drain. fill 16 oz wide mouthed jar until 3/4 - 4/5 full, place tyvek over top of jar then metal cap with small holes punched for inoculation ports, then screw on lid. place jars in pressure cooker for correct time (at least 90 minutes) and temp/psi (15 PSI) after completely cooled down then inoculate with syringe and place in incubator until colonization is complete. Usually there is no need for an incubator. What is the temperature in your room?

The spores dropped from a fruit have a very large range of genetics. The parent fruit doesn't really matter once it is stable enough. ( Unless your trying to cross varieties)
I used to just knock up grain with syringes all the time with great success. Then I started agar and now I feel dumb for not trying it earlier. You end up having many many more fruits per syringe then you would just knocking them up.
You also have the ability to see (most) contamination .
The list continues.
Utsf. Look up cloning and pastyplates.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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