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OfflineDabrit
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Looking for tips on agar transfers in terms of technique
    #23965451 - 12/29/16 12:39 PM (7 years, 1 month ago)

So I just made my very first agar transfers and holy shit balls it was way harder than I expected. Note to self don't get hopped up on caffeine prior to doing it my hands were super shaky.

I don't know if it's just because I'm working inside of a SAB instead of in front of a laminar flow hood or what but I found this to be the most difficult part of cultivation so far. I found it near impossible at times to get the small piece of mycelium to stick to the blade of the scalpel, my original plates look butchered as hell and obviously you can see I ended up cutting out a way bigger section than I intended.

Here take a look lol.



Anyone have any tips for making this a little easier in terms of technique? Or is experience and repetition the only way to get better at this? I feel like I just completed an open heart surgery.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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Offlineenlightenment
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit] * 1
    #23965460 - 12/29/16 12:43 PM (7 years, 1 month ago)

Make some agar without nutrients and pour it in used dishes or containers or something like that. You do not have to sterilize that of course. Exercise with this. Cut out a few pieces inside a SAB. You will get used to the movement and cutting process.

It looks like you cut from a strange angle. Try to cut from 60-90° position (scalpel).


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OfflineNDStepp84
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit] * 1
    #23965466 - 12/29/16 12:45 PM (7 years, 1 month ago)

Cut all the way through the agar and scoop out a wedge of myc covered agar or spear it with the scalpel  instead of just trying to remove the myc off the top of the agar.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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InvisiblemushboyMDiscord
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit] * 1
    #23965469 - 12/29/16 12:46 PM (7 years, 1 month ago)

practice. just practice.

and just as long as your are clean, who cares if you butcher a plate?

i have shaky hands myself and i am not artistic with a brush or anything.
make dummy petris to test your skillz. who cares if you waste a few cents worth of media?
better then the precious cultures being man handled with trail/error.

i do 'dummy' shit all the time. even dummy monos to practice making even substrate layers without being paranoid about my spawn being over handled.


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OfflineNDStepp84
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Re: Looking for tips on agar transfers in terms of technique [Re: mushboy]
    #23965478 - 12/29/16 12:50 PM (7 years, 1 month ago)

Looks like he's trying to cut the myc off the surface of the agar instead of cutting whole wedges out of the agar, that would be a PITA.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Offlineenlightenment
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Re: Looking for tips on agar transfers in terms of technique [Re: NDStepp84] * 1
    #23965484 - 12/29/16 12:52 PM (7 years, 1 month ago)

Yes but I already told him in another thread that he should cut down to the bottom :shrug:

Dabrit
What scalpel blade do you use? 11 is the most common for agar work. I also like to work with 12 sometimes.


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OfflineTerpfreak
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Re: Looking for tips on agar transfers in terms of technique [Re: enlightenment] * 1
    #23965491 - 12/29/16 12:55 PM (7 years, 1 month ago)

Its all practice. Youll do fine in no time


--------------------


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OfflineTh3Issu3
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Re: Looking for tips on agar transfers in terms of technique [Re: enlightenment] * 1
    #23965502 - 12/29/16 01:00 PM (7 years, 1 month ago)

I cut sort of a triangular shape. Then go to the tip of it and slice under, kind of bringing it up at the same time.

I usually get a decent pizza shaped piece stuck to the scalpel. Then on the receiving plate, I use the scalpel that the piece is on and kind of cut into the agar. It usually leaves it perfectly on the plate.


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OfflineNDStepp84
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Re: Looking for tips on agar transfers in terms of technique [Re: Terpfreak] * 1
    #23965511 - 12/29/16 01:03 PM (7 years, 1 month ago)

I shake like a chihuahua shitting a peach seed, bad nerves and surgery. Like he others said practice and don't worry about screwing up, you are only out like 10 cents a plate and if you do you can see it, that's what agar is for. Maybe limit the caffeine before transfers and pop a couple valarian.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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InvisiblemushboyMDiscord
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Re: Looking for tips on agar transfers in terms of technique [Re: NDStepp84]
    #23965513 - 12/29/16 01:04 PM (7 years, 1 month ago)

Quote:

NDStepp84 said:
a chihuahua shitting a peach seed.




i loled


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OfflineDabrit
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Re: Looking for tips on agar transfers in terms of technique [Re: enlightenment]
    #23965537 - 12/29/16 01:14 PM (7 years, 1 month ago)

Quote:

enlightenment said:
Make some agar without nutrients and pour it in used dishes or containers or something like that. You do not have to sterilize that of course. Exercise with this. Cut out a few pieces inside a SAB. You will get used to the movement and cutting process.

It looks like you cut from a strange angle. Try to cut from 60-90° position (scalpel).



Quote:

NDStepp84 said:
Cut all the way through the agar and scoop out a wedge of myc covered agar or spear it with the scalpel  instead of just trying to remove the myc off the top of the agar.



Quote:

NDStepp84 said:
Looks like he's trying to cut the myc off the surface of the agar instead of cutting whole wedges out of the agar, that would be a PITA.



Quote:

enlightenment said:
Yes but I already told him in another thread that he should cut down to the bottom :shrug:

Dabrit
What scalpel blade do you use? 11 is the most common for agar work. I also like to work with 12 sometimes.




I did actually try cutting to the bottom of the plates I think I disturbed some adjacent agar which may cover up this in the pics, duly noted about the 60-90 degree angle for cutting I was like you said trying to cut too parallel to the surface of the agar. I held the scalpel like a pen not sure if that's correct either.

Enlightenment I'm using a Scalpel handle #3 with #11 blades. Here's a pic:



--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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Invisiblefilthyknees
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit]
    #23965591 - 12/29/16 01:37 PM (7 years, 1 month ago)

It's harder for me to transfer a thinly poured plate than a thicker poured plate. Cannot tell very well from your pics, they don't look near full so maybe try pouring more agar per plate.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
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OfflineDabrit
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Re: Looking for tips on agar transfers in terms of technique [Re: filthyknees]
    #23965656 - 12/29/16 02:02 PM (7 years, 1 month ago)

Quote:

filthyknees said:
It's harder for me to transfer a thinly poured plate than a thicker poured plate. Cannot tell very well from your pics, they don't look near full so maybe try pouring more agar per plate.




Ok thanks for the tip.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineAK1000
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit]
    #23965777 - 12/29/16 02:52 PM (7 years, 1 month ago)

While we're on the topic... I got a side question: When cutting out a piece of agar with mycelium on it, when you go to put it on the new plate, do you like to make it face down (so the mycelium is facing down) or up or on its side or whatever the universe decides for you?  Sometimes I have plates that don't grow for whatever reason and I'm wondering if it's related to that, or if I'm just not getting a big enough piece?


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

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Invisiblefilthyknees
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Re: Looking for tips on agar transfers in terms of technique [Re: AK1000] * 1
    #23965779 - 12/29/16 02:54 PM (7 years, 1 month ago)

Whatever side


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineDabrit
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Re: Looking for tips on agar transfers in terms of technique [Re: AK1000]
    #23965781 - 12/29/16 02:55 PM (7 years, 1 month ago)

Quote:

AK1000 said:
While we're on the topic... I got a side question: When cutting out a piece of agar with mycelium on it, when you go to put it on the new plate, do you like to make it face down (so the mycelium is facing down) or up or on its side or whatever the universe decides for you?  Sometimes I have plates that don't grow for whatever reason and I'm wondering if it's related to that, or if I'm just not getting a big enough piece?




Honestly all my time and effort was spent just being able to get the piece of mycelium onto the scalpel to begin with when making the transfer I honestly didn't give a shit which way it faced when I placed it onto the new plate. 

This was my first time doing this though so I can't really comment if it matters or not. I doubt it. I'm sure someone else on here with more experience will be able to answer that for you


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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InvisiblemushboyMDiscord
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Re: Looking for tips on agar transfers in terms of technique [Re: AK1000]
    #23965785 - 12/29/16 02:55 PM (7 years, 1 month ago)

i just drop them and hope they land facing down. or i sloppily mash it in the agar.

but ive never had a transfer not take hold if it feel upside.. it gets all fuzzy
and branches out anyway.


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InvisibleMycolorado
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Re: Looking for tips on agar transfers in terms of technique [Re: mushboy]
    #23965827 - 12/29/16 03:14 PM (7 years, 1 month ago)

Face-down transfer is called "sandwiching" and is typically used to help isolate mycelium away from contams, the idea being that the sandwich will help impede the contam's progress while the mushroom mycelium grows out ahead for transfer.


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OfflineDabrit
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Re: Looking for tips on agar transfers in terms of technique [Re: Mycolorado]
    #23965872 - 12/29/16 03:33 PM (7 years, 1 month ago)

Quote:

Mycolorado said:
Face-down transfer is called "sandwiching" and is typically used to help isolate mycelium away from contams, the idea being that the sandwich will help impede the contam's progress while the mushroom mycelium grows out ahead for transfer.




:takingnotes:


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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InvisibleIntelligentxfruit
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit]
    #23965899 - 12/29/16 03:41 PM (7 years, 1 month ago)

I always try to cut rectangles, spear the rectangle favoring one end, the slide off using the longer end.

Think of the size like a piece of rice, spear one end, slide it off with the other


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OfflineTh3Issu3
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Re: Looking for tips on agar transfers in terms of technique [Re: Intelligentxfruit] * 2
    #23966000 - 12/29/16 04:19 PM (7 years, 1 month ago)

When transferring a agar wedge face up.

When fucking dem bitched face down!


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OfflineTiamo
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Re: Looking for tips on agar transfers in terms of technique [Re: enlightenment]
    #23966005 - 12/29/16 04:21 PM (7 years, 1 month ago)

Quote:

enlightenment said:
It looks like you cut from a strange angle. Try to cut from 60-90° position (scalpel).




It's bad practice to let your hand go above the plate right? That's why I've been having so much trouble doing transfers. My hand gets all weird and I'm scared to move it any way really.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

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InvisibleComebackKid
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Re: Looking for tips on agar transfers in terms of technique [Re: Intelligentxfruit]
    #23966006 - 12/29/16 04:22 PM (7 years, 1 month ago)

Take a shot of vodka or whisky before SAB work!

I like to use both hands when making a transfer.  I'll hold the plate up in one hand and tip it toward my scalpel while making my cuts. Feels more natural than trying to work with it flat on the table.

I like to cut little skinny oval slivers out if the leading edge. Small as possible.
I'll make two cuts starting from the same spot and ending in the same spot.
Before I pull the scalpel out of the agar after my second slice, I put pressure with the side of my scalpel and squeeze my wedge out of the agar and balance it on the flat of my scalpel.
Now I have a tiny agar flake laying on my scalpel blade which then I'll carefully move to the center of my next plate.
I'll then run my blade through the next plate leaving the agar wedge behind.

You can do it with squares or triangles as well just make sure after your final slice that you leave the blade in and put pressure on your wedge to pop it out and rest it on your blade :thumbup:

I used to have trouble with wedges sticking to my scalpel from squewering  them. I came up with this method after many frustrating transfers.
You'll find what works for you after working with it long enough. It's a pretty forgiving practice tbh. If you fuck it up now you'll be able to fix it in a few days after it grows out again :shrug:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleTookitooki
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Re: Looking for tips on agar transfers in terms of technique [Re: ComebackKid]
    #23966069 - 12/29/16 04:56 PM (7 years, 1 month ago)

I recently had the same issue as the OP, spent most of yesterday and today searching for a better way.  I resorted to scraping the myc off the agar.  I guess I didn't pay much attetion to the fact they were pulling a chunk of agar up with it. 

Now I'm in search of a good option for making liquid inoculate, with out sacrificing my whole agar dish.


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OfflineDabrit
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Re: Looking for tips on agar transfers in terms of technique [Re: ComebackKid]
    #23966078 - 12/29/16 05:02 PM (7 years, 1 month ago)

Quote:

ComebackKid said:
Take a shot of vodka or whisky before SAB work!

I like to use both hands when making a transfer.  I'll hold the plate up in one hand and tip it toward my scalpel while making my cuts. Feels more natural than trying to work with it flat on the table.

I like to cut little skinny oval slivers out if the leading edge. Small as possible.
I'll make two cuts starting from the same spot and ending in the same spot.
Before I pull the scalpel out of the agar after my second slice, I put pressure with the side of my scalpel and squeeze my wedge out of the agar and balance it on the flat of my scalpel.
Now I have a tiny agar flake laying on my scalpel blade which then I'll carefully move to the center of my next plate.
I'll then run my blade through the next plate leaving the agar wedge behind.

You can do it with squares or triangles as well just make sure after your final slice that you leave the blade in and put pressure on your wedge to pop it out and rest it on your blade :thumbup:

I used to have trouble with wedges sticking to my scalpel from squewering  them. I came up with this method after many frustrating transfers.
You'll find what works for you after working with it long enough. It's a pretty forgiving practice tbh. If you fuck it up now you'll be able to fix it in a few days after it grows out again :shrug:




Solid advice on technique old chap thanks. Unfortunately I'm taking a break from booze. But next time I'll transfer sans café


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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Offlinewild child
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit]
    #23966196 - 12/29/16 05:57 PM (7 years, 1 month ago)

ive yet to start Agar, however my question is...when going to put a wedge into a grain jar..is it okay to have agar on it or will that help contam the jar...basically should it be 100 myc..added or can there be some agar stuck to it?


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Re: Looking for tips on agar transfers in terms of technique [Re: wild child]
    #23966208 - 12/29/16 06:01 PM (7 years, 1 month ago)

"The tiger drop" is a method where one will drop a whole clean agar culture into a jar of grains.
Agar wedges will do fine too though :lol:

Edit: visuals


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


Edited by ComebackKid (12/29/16 06:04 PM)


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Offlinewild child
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Re: Looking for tips on agar transfers in terms of technique [Re: ComebackKid]
    #23966211 - 12/29/16 06:04 PM (7 years, 1 month ago)

gotcha thanks, btw...Ive been doing your water bottom tek and its working great.


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Re: Looking for tips on agar transfers in terms of technique [Re: wild child]
    #23966241 - 12/29/16 06:18 PM (7 years, 1 month ago)

Good to hear man! I'm glad people are still able to find that tek.

Hey Darbit! Chamomile tea is known to calm the nerves a bit. Might be a good alternative to the cafe and alcohol?
Good luck with your break man


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineDabrit
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Re: Looking for tips on agar transfers in terms of technique [Re: ComebackKid]
    #23966255 - 12/29/16 06:24 PM (7 years, 1 month ago)

Quote:

ComebackKid said:
Good to hear man! I'm glad people are still able to find that tek.

Hey Darbit! Chamomile tea is known to calm the nerves a bit. Might be a good alternative to the cafe and alcohol?
Good luck with your break man




Thanks man, yeah I think I do have some sleepytime tea kicking around somewhere which is basically just regular old chamomile. I'll pop on the kettle when I'm ready to transfer to grains.


--------------------
Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineKenetic
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit] * 1
    #23966304 - 12/29/16 06:48 PM (7 years, 1 month ago)

Try taking some kratom before you do any agar work.  It will relax the fuck out of you and before you know it you'll be doing agar in your sleep. 

My first time, I had a shirt tied around my face and was sweating balls and being clumsy, breathing hard and slightly shaking too!  I spilled agar on the sides of the dishes and was just all-around sloppy.  Now I just wear gloves and wipe down with iso and I'm good to go.  My heart-rate doesn't even change, I'm completely relaxed and confident. 
The key is to stay current and practice.  At one point I was doing about 50 plates a week and it was just routine then.  I did other stuff too like make blenderless li and noc up grains with agar or li.  Practice makes perfect

Ok recess is over kids.


--------------------
Todo Cambia
   

               
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Re: Looking for tips on agar transfers in terms of technique [Re: Kenetic]
    #24271655 - 04/25/17 08:25 PM (6 years, 9 months ago)

50 a week??! Where do you get your Petri dishes from and how much do you pay?!


--------------------
Speak to me in energy, that way I can understand you better.


ALSO...check out this fellow shroomites dope tunes...
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Re: Looking for tips on agar transfers in terms of technique [Re: mushroom_therapy] * 1
    #24271669 - 04/25/17 08:36 PM (6 years, 9 months ago)

check out sponsors I'm sure there is some good deals on sleaves of 100-500 dishes

or just go all out and buy 100 glad mini rounds. with transfers, i usually keep them around 2 weeks, so 100 will allow you to do 50 a week. at 50 cents a pop, thats $50 for more pasty plates than you will ever want to look at


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Re: Looking for tips on agar transfers in terms of technique [Re: Munchauzen]
    #24271723 - 04/25/17 09:02 PM (6 years, 9 months ago)

Thanks for the reply! Ive only used the glad minis but I have a hard time being able to see the mycellium clearly through these. I will check out sponsor sites, thanks!


--------------------
Speak to me in energy, that way I can understand you better.


ALSO...check out this fellow shroomites dope tunes...
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Re: Looking for tips on agar transfers in terms of technique [Re: mushroom_therapy]
    #24310563 - 05/10/17 06:41 PM (6 years, 8 months ago)

I'm thinking that pasty plates are the way to go now over regular Petris. It's just too much of a pain in the ass trying to pour agar inside of a SAB.

I'll wait until I move up in the world and have an actual HEPA Laminar flow hood.


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Invisiblejkz
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Registered: 06/16/13
Posts: 1,910
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit]
    #24310673 - 05/10/17 07:47 PM (6 years, 8 months ago)

What do you do if you don't have a scapel lol


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Offlinejeezy117
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Re: Looking for tips on agar transfers in terms of technique [Re: jkz]
    #24310694 - 05/10/17 07:55 PM (6 years, 8 months ago)

Thoroughly interested in this myself.

When you say 90 degrees do you mean holding the petri dish in your left hand at a 90 and cutting with your right?

I thought that the goal was to never have your hands hover over anything in a SAB?


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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Looking for tips on agar transfers in terms of technique [Re: jeezy117]
    #24310698 - 05/10/17 07:58 PM (6 years, 8 months ago)

Quote:

jeezy117 said:
Thoroughly interested in this myself.

When you say 90 degrees do you mean holding the petri dish in your left hand at a 90 and cutting with your right?

I thought that the goal was to never have your hands hover over anything in a SAB?



why use words when I can just show you



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Offlinejeezy117
FunGuy
I'm a teapot


Registered: 05/31/09
Posts: 100
Last seen: 5 years, 8 months
Re: Looking for tips on agar transfers in terms of technique [Re: Munchauzen]
    #24312506 - 05/11/17 05:20 PM (6 years, 8 months ago)

Quote:

Munchauzen said:
Quote:

jeezy117 said:
Thoroughly interested in this myself.

When you say 90 degrees do you mean holding the petri dish in your left hand at a 90 and cutting with your right?

I thought that the goal was to never have your hands hover over anything in a SAB?



why use words when I can just show you






Way to go and making it look simple.

:asianofapproval:


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