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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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PEU syringe issue
#23965220 - 12/29/16 10:53 AM (7 years, 1 month ago) |
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I bought a peu and ape syringe a while back (6 months maybe) I put both to agar the ape always worked fine but out of 20 plates of PEU only two got any growth and for some reason once I got to transfer 2 they also stopped growing. What could be the issue here I use premade bags of pda thinking im gonna knock up my own MEA and try that as none of my premix agar dishes ever seem as good as everyone elses plates.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I recently picked up a PEU syringe that didn't germinate on agar. I ended up shooting some spores into an LC made with 1 gram LME, 0.5 grams of dextrose aND 700ml of water. It had a good amount of growth after about 10 days. At that point I put some of the LC on agar and it took off with good strong growth. Might work for you.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: PEU syringe issue [Re: MudaFuka]
#23965263 - 12/29/16 11:13 AM (7 years, 1 month ago) |
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Yeah sweet ill give that a shot just need some dextrose got lme. The only growth I had was after a month which I transfered once that took ages to get going then I transfered again and it stalled.
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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You don't need dextrose.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: PEU syringe issue [Re: MudaFuka]
#23965337 - 12/29/16 11:46 AM (7 years, 1 month ago) |
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Sweet ive just finished pcing some mea so once thats cooled down ill knock up an lc
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Good luck.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: PEU syringe issue [Re: MudaFuka]
#23965895 - 12/29/16 03:40 PM (7 years, 1 month ago) |
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Thanks mate
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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