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Offlinemrmazdarx9
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Registered: 05/15/16
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PEU syringe issue
    #23965220 - 12/29/16 10:53 AM (7 years, 1 month ago)

I bought a peu and ape syringe a while back (6 months maybe) I put both to agar the ape always worked fine but out of 20 plates of PEU only two got any growth and for some reason once I got to transfer 2 they also stopped growing. What could be the issue here I use premade bags of pda thinking im gonna knock up my own MEA and try that as none of my premix agar dishes ever seem as good as everyone elses plates.


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InvisibleMudaFuka
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Re: PEU syringe issue [Re: mrmazdarx9]
    #23965258 - 12/29/16 11:10 AM (7 years, 1 month ago)

I recently picked up a PEU syringe that didn't germinate on agar. I ended up shooting some spores into an LC made with 1 gram LME, 0.5 grams of dextrose aND 700ml of water. It had a good amount of growth after about 10 days. At that point I put some of the LC on agar and it took off with good strong growth. Might work for you. :shrug:


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Offlinemrmazdarx9
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Re: PEU syringe issue [Re: MudaFuka]
    #23965263 - 12/29/16 11:13 AM (7 years, 1 month ago)

Yeah sweet ill give that a shot just need some dextrose got lme. The only growth I had was after a month which I transfered once that took ages to get going then I transfered again and it stalled.


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InvisibleMudaFuka
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Re: PEU syringe issue [Re: mrmazdarx9]
    #23965329 - 12/29/16 11:40 AM (7 years, 1 month ago)

You don't need dextrose.


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Offlinemrmazdarx9
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Re: PEU syringe issue [Re: MudaFuka]
    #23965337 - 12/29/16 11:46 AM (7 years, 1 month ago)

Sweet ive just finished pcing some mea so once thats cooled down ill knock up an lc


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InvisibleMudaFuka
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Re: PEU syringe issue [Re: mrmazdarx9]
    #23965850 - 12/29/16 03:24 PM (7 years, 1 month ago)

Good luck.:thumbup:


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Offlinemrmazdarx9
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Re: PEU syringe issue [Re: MudaFuka]
    #23965895 - 12/29/16 03:40 PM (7 years, 1 month ago)

Thanks mate


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