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OfflineDabrit
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Question regarding agar culture transfer
    #23962914 - 12/28/16 02:36 PM (7 years, 1 month ago)

So my ms inoculated petri's are reading for transfers. I'm currently prepping new agar for the transfers.

I am wondering when you cut the small section out of a leading edge of mycelium do you cut down all the way to the bottom of the agar and then transfer to a new plate? Or do you just cut away at at the surface level of the mycelium?

:shrug:


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Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




Edited by Dabrit (12/28/16 02:37 PM)


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Offlineolskoolgrower
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Re: Question regarding agar culture transfer [Re: Dabrit]
    #23962917 - 12/28/16 02:37 PM (7 years, 1 month ago)

cut all the way down.  Your trying to pick up a full wedge.


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Offlineenlightenment
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Re: Question regarding agar culture transfer [Re: olskoolgrower]
    #23962927 - 12/28/16 02:40 PM (7 years, 1 month ago)

Cut it down and poke the piece of agar gently with your scalpel blade.
Lift it up and transfer it to another dish. It does not matter if the uncolonized side is placed on the new dish. The mycelium starts to regenerate about 2-3 days after cutting and will continue to grow.
I like to cut triangles. It is fast because you just need 3 cuts. But everyone has his own preferences.


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OfflineDabrit
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Re: Question regarding agar culture transfer [Re: enlightenment]
    #23962949 - 12/28/16 02:46 PM (7 years, 1 month ago)

Quote:

olskoolgrower said:
cut all the way down.  Your trying to pick up a full wedge.



Quote:

enlightenment said:
Cut it down and poke the piece of agar gently with your scalpel blade.
Lift it up and transfer it to another dish. It does not matter if the uncolonized side is placed on the new dish. The mycelium starts to regenerate about 2-3 days after cutting and will continue to grow.
I like to cut triangles. It is fast because you just need 3 cuts. But everyone has his own preferences.




Ok thanks guys.

Enlightenment you are very helpful I must say. I'd say the vast majority of questions I've had in the past few weeks have been answered by yourself.  You are a gentleman and a scholar!


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Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineJabensis
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Re: Question regarding agar culture transfer [Re: enlightenment]
    #23962958 - 12/28/16 02:49 PM (7 years, 1 month ago)

i just tried to get a rice grain sized sample from the leading edge... never tried to go to the bottom of the dish.  All you need is a tiny piece of mycelium.


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InvisiblemushboyMDiscord
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Re: Question regarding agar culture transfer [Re: Dabrit]
    #23962960 - 12/28/16 02:50 PM (7 years, 1 month ago)

the piece will also get really fuzzy for a day or so. its normal.

i am brutal when i take cuts. i have shaky hands and im clumsy by nature but
i try my best to be as quick and cafeful as i can and never skimp on being clean.


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OfflineDabrit
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Re: Question regarding agar culture transfer [Re: mushboy]
    #23962964 - 12/28/16 02:52 PM (7 years, 1 month ago)

Quote:

mushboy said:
the piece will also get really fuzzy for a day or so. its normal.

i am brutal when i take cuts. i have shaky hands and im clumsy by nature but
i try my best to be as quick and cafeful as i can and never skimp on being clean.




Ok good to know. No doubt I would have seen the fuzz and started thinking mold.

#Noob


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Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume :wink:




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OfflineWicked Burn
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Re: Question regarding agar culture transfer [Re: enlightenment]
    #23962985 - 12/28/16 02:59 PM (7 years, 1 month ago)

I recently tried something a little different on my last round of transfers.  I used an inoculation loop to just scrape a small amount of mycelium from the surface of the agar then I just sliced the loop into the receiving plates agar.  For most of the transfers I couldn't even see any myc on the loop but surprisingly, to me anyway, it worked on 9 out of 10 transfers.  I think this may be how I do my transfers from here on out.



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Mr. Pink:  "Hey, why am I Mr. Pink?"
Joe:  "Because you're a faggot."
                                     
                                                    -Reservoir Dogs


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InvisiblemushboyMDiscord
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Re: Question regarding agar culture transfer [Re: Wicked Burn]
    #23962990 - 12/28/16 03:03 PM (7 years, 1 month ago)

nice


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