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InvisibleZiran
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Diving In.
    #23962805 - 12/28/16 01:41 PM (7 years, 1 month ago)

Just got this in the mail today. Gonna be diving into agar finally.



gonna do up some plates like Doc_T using the small glass jars.

Doc_T does Agar


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.



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OfflineEdmunter
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Re: Diving In. [Re: Ziran]
    #23962938 - 12/28/16 02:43 PM (7 years, 1 month ago)



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OfflineJabensis
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Re: Diving In. [Re: Ziran]
    #23962947 - 12/28/16 02:46 PM (7 years, 1 month ago)

those Doc_T "plates" go by the name of "no-pours"
Thats where i started and have had great success with them... really easy to work with.

I liked using the wide mouth smaller flat jelly jars personally.

Have you decided on a recipe for your agar yet?

My recommendation is to simmer the agar mixture for a bit to homogenize everything prior to pouring the agar mix into the jars.

If you don't have shrooms going right meow to clone from and are using spore solution, I recommend only using a few drops of solution.


The advantage of Agar as you probably know is the ability to identify con tams early and to isolate away from them to other Agar dishes.

Use a Still Air Box or SAB to do your agar work.. and always practice impeccable sterile technique.

The pic on my sig is a no-pour.


--------------------
J


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Invisiblefilthyknees
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Re: Diving In. [Re: Edmunter]
    #23963421 - 12/28/16 05:52 PM (7 years, 1 month ago)

Quote:

Edmunter said:
Try Pastys easy agar, its better.

https://www.shroomery.org/forums/showflat.php/Number/19219906#19219906



better?... visibility with the glass is better than pp5 anyways :shrug: :lol:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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InvisibleMunchauzen
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Re: Diving In. [Re: filthyknees]
    #23963522 - 12/28/16 06:31 PM (7 years, 1 month ago)

Quote:

filthyknees said:
Quote:

Edmunter said:
Try Pastys easy agar, its better.

https://www.shroomery.org/forums/showflat.php/Number/19219906#19219906



better?... visibility with the glass is better than pp5 anyways :shrug: :lol:



both have pros and cons. you can't do a tiger drop with glass. glass has better visibility. plastic lids pop off with one finger. jar lids have to be unscrewed and lifted off....


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InvisibleZiran
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Re: Diving In. [Re: Jabensis]
    #23963538 - 12/28/16 06:38 PM (7 years, 1 month ago)

I have not. Was thinking potato flakes, karo or honey, and some food coloring.

What do you think munch? I already have the jars XD


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.



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OfflineKenetic
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Re: Diving In. [Re: Ziran]
    #23963542 - 12/28/16 06:41 PM (7 years, 1 month ago)

I love the idea that you can just squeeze a pasty plate into a spawn jar.....
And fruiting invitro.....

But damn I love my petris.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflinePinPornProducer
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Re: Diving In. [Re: Kenetic]
    #23963557 - 12/28/16 06:47 PM (7 years, 1 month ago)

Honestly I don't know how tiger dropping became a thing, we don't like the mycelium to cover the entire plate for bacterial reasons so we colonize 75% of the surface and cut the myc out the center. But it's okay to drop the entire agar puck into a jar :lol: :shrug:


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OfflineKenetic
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Re: Diving In. [Re: PinPornProducer]
    #23963561 - 12/28/16 06:49 PM (7 years, 1 month ago)

Yeah I thought the same thing.  I always cut my colonized agar out because I'm paranoid there is bacteria or something near the edge, and the edge is the part that contams on me most of the time (but rarely get contams anyway)


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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InvisibleMunchauzen
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Re: Diving In. [Re: Ziran]
    #23963564 - 12/28/16 06:50 PM (7 years, 1 month ago)

Quote:

Ziran said:
I have not. Was thinking potato flakes, karo or honey, and some food coloring.

What do you think munch? I already have the jars XD



easy to remember agar recipe in my signature. good for spore germination.


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InvisibleMunchauzen
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Re: Diving In. [Re: PinPornProducer]
    #23963573 - 12/28/16 06:51 PM (7 years, 1 month ago)

Quote:

PinPornProducer said:
Honestly I don't know how tiger dropping became a thing, we don't like the mycelium to cover the entire plate for bacterial reasons so we colonize 75% of the surface and cut the myc out the center. But it's okay to drop the entire agar puck into a jar :lol: :shrug:



who is this we you speak of? I mean I haven't seen many people preaching that practice. and what, exactly, are your "bacterial reasons?"


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OfflinePinPornProducer
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Re: Diving In. [Re: Munchauzen]
    #23963579 - 12/28/16 06:54 PM (7 years, 1 month ago)

It's like general knowledge on this site :shrug: countless ppl are in agreement on this...


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InvisibleZiran
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Re: Diving In. [Re: Munchauzen]
    #23963608 - 12/28/16 07:04 PM (7 years, 1 month ago)

Oh shit. I just realized. Instead of tiger dropping I can use your LI method with those glass jars.


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.



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InvisibleMunchauzen
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Re: Diving In. [Re: PinPornProducer]
    #23963612 - 12/28/16 07:06 PM (7 years, 1 month ago)

Quote:

PinPornProducer said:
It's like general knowledge on this site :shrug: countless ppl are in agreement on this...



I guess its just outdated info then. if there was any actual concerns, then tiger dropping and blenderless LI wouldn't work. yet, they do. proof is in the pudding.


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Invisibler.lutece
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Re: Diving In. [Re: Munchauzen]
    #23963675 - 12/28/16 07:35 PM (7 years, 1 month ago)

My understanding was that a plate grown all the way to the edge has a higher chance of getting contaminated when handled improperly, but that correct sterile procedure should vastly reduce the likelihood to near non-existence.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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InvisibleZiran
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Re: Diving In. [Re: Ziran]
    #23987977 - 01/06/17 10:22 PM (7 years, 24 days ago)

Going to continue this thread in my journal.

Agar


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.



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OfflinecronicrFacebook
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Re: Diving In. [Re: PinPornProducer]
    #23987986 - 01/06/17 10:25 PM (7 years, 24 days ago)

Quote:

PinPornProducer said:
Honestly I don't know how tiger dropping became a thing, we don't like the mycelium to cover the entire plate for bacterial reasons so we colonize 75% of the surface and cut the myc out the center. But it's okay to drop the entire agar puck into a jar :lol: :shrug:



why would you take myc from the center?


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinePinPornProducer
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Re: Diving In. [Re: cronicr]
    #23988000 - 01/06/17 10:32 PM (7 years, 24 days ago)

No, cut all the myc out but just not letting the myc grow out till it touches the glass


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OfflinecronicrFacebook
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Re: Diving In. [Re: PinPornProducer]
    #23988014 - 01/06/17 10:35 PM (7 years, 24 days ago)

oh i see i thought you were implying to avoid the leading edge:lol:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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