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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
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Re: Chicon Nindo and tampanensis [Re: Edmunter]
#23960870 - 12/27/16 04:02 PM (7 years, 2 months ago) |
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Shot 3 looks like a stone just up and right of center. I'd dig that up and transfer it as well as any area showing the darkest brown hard growth visible from the bottom of the plate. So far the CN stones grow under the surface like your plate appears to be doing and the tamp stones grow on top.
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olskoolgrower
Pro Grower

Registered: 12/27/16
Posts: 57
Last seen: 6 years, 9 months
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
#23960950 - 12/27/16 04:44 PM (7 years, 2 months ago) |
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I was given a print by azure when he and elfstone first released CN Mex. I never had alot of luck with stones from it. P. Tamp has always been hit and miss for stone production for me also. Sometimes ill get 200g wet after 3 months sometimes 30-40 from MS You just seem to never know with stone producers with the exception of P. Galindoi large sclerotia. If you want stones id suggest doing the agar work on that species
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Mycolorado
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Registered: 07/23/16
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I've been transferring the stones with the goal of propagating those traits. Hopefully, it pays off, though I'm not sure how much nature v nurture plays in stone formation. My gut is leaning toward nurture.
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olskoolgrower
Pro Grower


Registered: 12/27/16
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
#23961015 - 12/27/16 05:26 PM (7 years, 2 months ago) |
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Quote:
Mycolorado said: I've been transferring the stones with the goal of propagating those traits. Hopefully, it pays off, though I'm not sure how much nature v nurture plays in stone formation. My gut is leaning toward nurture.
Get some different stone producer MS syringes and grow them under the same conditions. Im sure that after that you will change your mind...
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
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excellent! Post's like these really make me want to try some stones for sure. And throughout my whole agar career I've never had a plate fruit on me. Really surprised you got some stones!
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
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These were started from prints with 3 CN colonies developing on a plate and 1 tamp colony on another. At this point, all 3 CN colonies have produced at least one stone and the tamp colony has produced 3 large stones. I have no experience with stone producers so nothing practical to base the assumption on but Stamets says stone production is best on 4.5% MEA grown in the dark. That with the fact that sclerotia are a strategy for surviving unfavorable conditions makes me lean towards nurture. Then again, maybe I was fortunate and got good stone-producing genetics.
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Kenetic
Nam Sayin



Registered: 08/24/14
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Re: Chicon Nindo and tampanensis [Re: Mycolorado]
#23961109 - 12/27/16 06:26 PM (7 years, 2 months ago) |
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well you're doing something right for sure
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
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Re: Chicon Nindo and tampanensis [Re: Kenetic]
#23961756 - 12/28/16 01:33 AM (7 years, 2 months ago) |
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Quote:
kenetic said: excellent! Post's like these really make me want to try some stones for sure. And throughout my whole agar career I've never had a plate fruit on me. Really surprised you got some stones!

Thats strange a nearly every plate I have ,if clean ,fruits at some point if I just leave it
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Edmunter
Mr



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Re: Chicon Nindo and tampanensis [Re: Edmunter]
#23961770 - 12/28/16 01:46 AM (7 years, 2 months ago) |
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Definitely something going on under the surface
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Kenetic
Nam Sayin



Registered: 08/24/14
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Re: Chicon Nindo and tampanensis [Re: Edmunter]
#23962195 - 12/28/16 08:54 AM (7 years, 2 months ago) |
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Do you use petris or pastybplates edmunter
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Edmunter
Mr



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Re: Chicon Nindo and tampanensis [Re: Kenetic]
#23962204 - 12/28/16 09:01 AM (7 years, 2 months ago) |
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Pasty easys
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Kenetic
Nam Sayin



Registered: 08/24/14
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Re: Chicon Nindo and tampanensis [Re: Edmunter]
#23962557 - 12/28/16 11:41 AM (7 years, 2 months ago) |
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Invitro shrooms seem to love those pasty plates because of all the extra air and room inside, makes a nice little microclimate, but petris don't have all the room and air and stuff. My agar dries before I see knots. Some people get invitro on plates but Im not sure how. Maybe I just havent kept them long enough
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Edmunter
Mr



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Re: Chicon Nindo and tampanensis [Re: Kenetic]
#23962570 - 12/28/16 11:49 AM (7 years, 2 months ago) |
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Its the quickest way to get a decent growing culture IMO. Spores plate-pin-plate-fruit. I also take transfers from the original plate too.
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enlightenment
alchemist


Registered: 08/09/09
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Re: Chicon Nindo and tampanensis [Re: Kenetic]
#23962571 - 12/28/16 11:49 AM (7 years, 2 months ago) |
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Usually I see pins after 2 weeks of full colonization of the agar dish. (not on every dish. About 20% of cubensis dishes produce invitro pins IME)
I use parafilm. It prevents the agar from drying too fast. Maybe this is the reason. 
If you use parafilm, too. I don't know why you don't get invitro pins.
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Kenetic
Nam Sayin



Registered: 08/24/14
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Who knows. Not a big priority for me anyway besides the novelty aspect.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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PlantManBee
undifferentiated



Registered: 11/17/16
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Re: Chicon Nindo and tampanensis [Re: Kenetic]
#23964836 - 12/29/16 07:55 AM (7 years, 2 months ago) |
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Looks great!
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Edmunter
Mr



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Re: Chicon Nindo and tampanensis [Re: Kenetic]
#23964898 - 12/29/16 08:29 AM (7 years, 2 months ago) |
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Quote:
kenetic said: Who knows. Not a big priority for me anyway besides the novelty aspect.
But t is a way to get a sterile pin with all the genetics you need for fruiting and growing fast.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
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Re: Chicon Nindo and tampanensis [Re: Edmunter]
#23964905 - 12/29/16 08:37 AM (7 years, 2 months ago) |
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I agree that there are quite a few advantages, including less cell division but I have enough going on that I am not pressed for time nor do I have any issues with taking biopsy samples for cloning. I'd rather just fruit a small ms grow and clone from there.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Edmunter
Mr



Registered: 05/01/13
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Re: Chicon Nindo and tampanensis [Re: Kenetic]
#23964908 - 12/29/16 08:39 AM (7 years, 2 months ago) |
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Fair play, I do both. For certain species that are hard to fruit I find this gives you a headstart
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Edmunter
Mr



Registered: 05/01/13
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Re: Chicon Nindo and tampanensis [Re: Edmunter]
#23964912 - 12/29/16 08:40 AM (7 years, 2 months ago) |
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I have taken 2 stones from my original spore plate now and transferred them and placed them in the dark.
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