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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Shiitake LC->agar problems
#23962185 - 12/28/16 08:50 AM (7 years, 1 month ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 01:03 PM)
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RolledUhhp
Amateur Cultivator

Registered: 08/10/16
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Re: Shiitake LC->agar problems [Re: amidogen]
#23962984 - 12/28/16 02:59 PM (7 years, 1 month ago) |
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Perhaps try a different agar recipe, as well as noc'ing up some cakes just to see what happens.
I don't much experience with agar, but for nothing at all to be growing seems very odd.
This might sound far fetched, but as a test perhaps you could make an extra plate this time around, and introduce a sample from your sink/toilet/shower ect.. I'd say to do this away from your work area, since sterility certainly isn't an issue with this plate.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Shiitake LC->agar problems [Re: RolledUhhp]
#23963640 - 12/28/16 07:22 PM (7 years, 1 month ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (06/21/18 11:26 AM)
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Pastywhyte
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Shiitake LC->agar problems [Re: amidogen]
#23963837 - 12/28/16 08:35 PM (7 years, 1 month ago) |
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Putting LC on a plate that grows nothing isn't indicative of contams, the contams would then show up and take over. Instead I would suspect the culture had maybe died.
When ordering cultures I would choose a wedge over LC. LC doesn't store well IME.
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Quadman
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Registered: 04/23/16
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Re: Shiitake LC->agar problems [Re: amidogen]
#23963843 - 12/28/16 08:38 PM (7 years, 1 month ago) |
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this is why I try to avoid LC. Really difficult to tell what you have. It's possible you've got two bad syringes. I grow Shiitake on potato flakes so it should work.
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RolledUhhp
Amateur Cultivator

Registered: 08/10/16
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Re: Shiitake LC->agar problems [Re: Pastywhyte]
#23963980 - 12/28/16 09:35 PM (7 years, 1 month ago) |
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This is along the line of thinking I had. Shouldn't SOMETHING grow? That's why I suggested introducing a contam that should grow rapidly, it seems to me the media isn't supporting life at all, no?
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Pastywhyte
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Registered: 09/15/12
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Re: Shiitake LC->agar problems [Re: RolledUhhp]
#23963999 - 12/28/16 09:43 PM (7 years, 1 month ago) |
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Well give the recipe you gave, that media is more than capable of growing fungi as well as contams.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Shiitake LC->agar problems [Re: Pastywhyte]
#23964519 - 12/29/16 01:53 AM (7 years, 1 month ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (06/21/18 11:26 AM)
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RolledUhhp
Amateur Cultivator

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Re: Shiitake LC->agar problems [Re: Pastywhyte]
#23966092 - 12/29/16 05:08 PM (7 years, 1 month ago) |
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Quote:
Pastywhyte said: Well give the recipe you gave, that media is more than capable of growing fungi as well as contams.
Wouldn't this lead us to believe that for some reason this batch is not offering something(s) needed for some type of life to grow?
I can think of a few scenario's, each as likely or unlikely as the next.
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Quadman
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Re: Shiitake LC->agar problems [Re: RolledUhhp]
#23966137 - 12/29/16 05:27 PM (7 years, 1 month ago) |
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There is nothing you can do to a standard agar recipe to screw up ,short of not following the recipe using some weird other ingredients. Mycelium is most likely dead or none was put on the plate.
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Pastywhyte
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Registered: 09/15/12
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Re: Shiitake LC->agar problems [Re: RolledUhhp]
#23966138 - 12/29/16 05:29 PM (7 years, 1 month ago) |
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Not really. There are plenty of nutes there. Even with starved agar recipes you should see some recovery if the LC was viable.
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RolledUhhp
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Re: Shiitake LC->agar problems [Re: Quadman]
#23966180 - 12/29/16 05:51 PM (7 years, 1 month ago) |
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Quote:
Quadman said: There is nothing you can do to a standard agar recipe to screw up ,short of not following the recipe using some weird other ingredients. Mycelium is most likely dead or none was put on the plate.
I feel like I might be beating a dead horse, and forgive me if I'm missing something obvious, but if it were just a case of the LC being bad wouldn't we still see something beginning to grow?
Again, I don't have much experience with agar, but would something like overcooking, or something silly like alcohol/bleach/soap ect.. not being fully rinsed from a container render the agar "non-nutritious" or something comparable? I just can't wrap my head around NOTHING growing in what is supposed to be lobster to all these microscopic beasties.
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Pastywhyte
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Shiitake LC->agar problems [Re: RolledUhhp]
#23966381 - 12/29/16 07:17 PM (7 years, 1 month ago) |
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If the LC was clean but the culture was dead then nothing would grow at all. LC not growing anything contams or mycelium means nothing in it was alive.
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RolledUhhp
Amateur Cultivator

Registered: 08/10/16
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Re: Shiitake LC->agar problems [Re: Pastywhyte]
#23966398 - 12/29/16 07:26 PM (7 years, 1 month ago) |
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I was thinking of it like spawning to bulk, where you are trying to race the contams to ensure the mushroom will win, but there should be no contams at all in our dishes, which explains why nothing would grow.
Thanks for helping me through it!
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