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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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14 days after g2g MS one shake... should I worry?
#23960775 - 12/27/16 03:12 PM (7 years, 1 month ago) |
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: 14 days after g2g MS one shake... should I worry? [Re: mushhiehunter]
#23960779 - 12/27/16 03:14 PM (7 years, 1 month ago) |
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Could you post a picture of your lid?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: 14 days after g2g MS one shake... should I worry? [Re: mushhiehunter]
#23960782 - 12/27/16 03:15 PM (7 years, 1 month ago) |
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yes that's toast, after g2g it should take a week or less
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
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Re: 14 days after g2g MS one shake... should I worry? [Re: bodhisatta]
#23960784 - 12/27/16 03:16 PM (7 years, 1 month ago) |
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It might just have no oxygen to grow
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Kenetic
Nam Sayin



Registered: 08/24/14
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Re: 14 days after g2g MS one shake... should I worry? [Re: enlightenment]
#23960816 - 12/27/16 03:29 PM (7 years, 1 month ago) |
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Looks good but two weeks?
Something is going on for sure. Did you use like 2 grains or something like that lol
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
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Re: 14 days after g2g MS one shake... should I worry? [Re: Kenetic] 1
#23960823 - 12/27/16 03:32 PM (7 years, 1 month ago) |
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I want to see the lid because lately I have seen two people without filters in their lids.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
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Re: 14 days after g2g MS one shake... should I worry? [Re: enlightenment]
#23960830 - 12/27/16 03:37 PM (7 years, 1 month ago) |
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Even without a filtered lid a jar will still colonize in under 2 weeks. And if it were to stall because of lack of ge the jar would still get at least 75-80% colonized before that would be an issue
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PinPornProducer
Buy the ticket, take the ride



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Re: 14 days after g2g MS one shake... should I worry? [Re: PinPornProducer]
#23960838 - 12/27/16 03:41 PM (7 years, 1 month ago) |
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Imo he prepped his grain to dry
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
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Re: 14 days after g2g MS one shake... should I worry? [Re: PinPornProducer]
#23960841 - 12/27/16 03:42 PM (7 years, 1 month ago) |
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Could take this long if the temps were very low. I don't see any contams..  Grains look swollen and hydrated to me.
Would also like to see top of jars.
-------------------- ¯\_(ツ)_/¯
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
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Re: 14 days after g2g MS one shake... should I worry? [Re: Tmethyl]
#23960853 - 12/27/16 03:49 PM (7 years, 1 month ago) |
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Also, I don't do g2g very often (prefer blenderless li) but I always shake right at inoculation, then at 30% or so.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
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Re: 14 days after g2g MS one shake... should I worry? [Re: Tmethyl] 1
#23960855 - 12/27/16 03:51 PM (7 years, 1 month ago) |
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Quote:
And if it were to stall because of lack of ge the jar would still get at least 75-80%
You know I like you but...

People had stalled jars because they used .22 syringe filters for their grain jars. And if .22 syringe filter failed for GE, a lid without a filter stalls the growth earlier.
https://www.shroomery.org/forums/showflat.php/Number/18436779
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
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Re: 14 days after g2g MS one shake... should I worry? [Re: enlightenment] 1
#23960872 - 12/27/16 04:03 PM (7 years, 1 month ago) |
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And I like you but...
Even unmodified lids colonize waaay better than what he's got for growth after 2 weeks
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olskoolgrower
Pro Grower

Registered: 12/27/16
Posts: 57
Last seen: 6 years, 8 months
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Re: 14 days after g2g MS one shake... should I worry? [Re: PinPornProducer]
#23960915 - 12/27/16 04:29 PM (7 years, 1 month ago) |
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For over 7 years I grew cubes with lids with out holes for GE. I can never think of a time that I had stalled jars. 2 weeks for a G2G is too long probably some sort of bacterial contam
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PinPornProducer
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Registered: 08/23/14
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Re: 14 days after g2g MS one shake... should I worry? [Re: olskoolgrower] 1
#23960932 - 12/27/16 04:33 PM (7 years, 1 month ago) |
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G2g should take 7-10 days to colonize and maybe another day or 2 with unmodified lids
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cronicr



Registered: 08/07/11
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Loc: Van Isle
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Re: 14 days after g2g MS one shake... should I worry? [Re: PinPornProducer] 1
#23960949 - 12/27/16 04:44 PM (7 years, 1 month ago) |
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How many jars did you g2g...if i made 3 from one it would take a few days...if i made 20 from one it would take longer
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Tmethyl
Smear in the shale


Registered: 07/16/12
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Re: 14 days after g2g MS one shake... should I worry? [Re: cronicr] 1
#23961040 - 12/27/16 05:40 PM (7 years, 1 month ago) |
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Quote:
cronicr said: How many jars did you g2g...if i made 3 from one it would take a few days...if i made 20 from one it would take longer
I remember when I first started doing bulk I g2g'ed one quart jar into 54 quart jars.
And it worked.  But yeah it took 10+ days.
-------------------- ¯\_(ツ)_/¯
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Sirtalis


Registered: 10/05/16
Posts: 409
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Re: 14 days after g2g MS one shake... should I worry? [Re: Tmethyl]
#23961047 - 12/27/16 05:45 PM (7 years, 1 month ago) |
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Could be low temps or the species. Ps. allenii takes me 2 weeks for g2g to finish. House temps are 60-65.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
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Re: 14 days after g2g MS one shake... should I worry? [Re: Sirtalis]
#23961068 - 12/27/16 05:58 PM (7 years, 1 month ago) |
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lid
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: 14 days after g2g MS one shake... should I worry? [Re: Kenetic] 1
#23961071 - 12/27/16 06:00 PM (7 years, 1 month ago) |
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could also be a shitty culture. OP did not include any details?
was the master jar slow as fuck? recover from shake? ...not 100% colonized when xfered???
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mycoshroom1994
scientist



Registered: 12/10/15
Posts: 322
Last seen: 5 years, 8 months
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Re: 14 days after g2g MS one shake... should I worry? [Re: Tmethyl]
#23961106 - 12/27/16 06:23 PM (7 years, 1 month ago) |
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Quote:
Tmethyl said:
Quote:
cronicr said: How many jars did you g2g...if i made 3 from one it would take a few days...if i made 20 from one it would take longer
I remember when I first started doing bulk I g2g'ed one quart jar into 54 quart jars.
And it worked.  But yeah it took 10+ days.
54 quarts. Jeez ur doin it right from the very beginning wernt u hahaha. Here's mine 24 hours later. Today. Sensi star Just beginning to recover. Some strains are much faster keep in mind!
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
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Re: 14 days after g2g MS one shake... should I worry? [Re: mycoshroom1994]
#23961110 - 12/27/16 06:26 PM (7 years, 1 month ago) |
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OP is as slow as his jars.
Edited by Tmethyl (12/27/16 06:57 PM)
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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Re: 14 days after g2g MS one shake... should I worry? [Re: PinPornProducer]
#23965578 - 12/29/16 01:31 PM (7 years, 1 month ago) |
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Quote:
PinPornProducer said: OP is as slow as his jars. 
true... I'm sorry guys for getting so late to you, I have quite large 1/2 inch holes with tyvek as filters so the AE shouldn't be the problem. the original jar was about 1 month old (should have mentioned that, sorry) and it did take about 20 days to colonize. anyway I decided to shake all the jars. I could smell nice fungal smell... will update in a few days. .
Edited by mushhiehunter (12/29/16 01:32 PM)
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
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Re: 14 days after g2g MS one shake... should I worry? [Re: mushhiehunter]
#23965826 - 12/29/16 03:13 PM (7 years, 1 month ago) |
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The real question here is, even if it was really slow growing strain. It beeing a MS from agar wouldn't the fastest growing strain take over anyway?? I'm guessing something is going wrong anyway...
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enlightenment
alchemist



Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: 14 days after g2g MS one shake... should I worry? [Re: mushhiehunter]
#23965838 - 12/29/16 03:18 PM (7 years, 1 month ago) |
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Did you use coffee in your soaking water? How did you prepare your grain jars?
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
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Re: 14 days after g2g MS one shake... should I worry? [Re: enlightenment]
#23965901 - 12/29/16 03:41 PM (7 years, 1 month ago) |
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Quote:
enlightenment said: Did you use coffee in your soaking water? How did you prepare your grain jars?
no coffee used, 6 hours soak 15 minutes simmer dried till felt dry to touch (approx 20 min drying by pouring the grain from strainer to strainer)
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enlightenment
alchemist



Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: 14 days after g2g MS one shake... should I worry? [Re: mushhiehunter]
#23965935 - 12/29/16 03:53 PM (7 years, 1 month ago) |
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Strange... Could you post a pic of your lids please? I know you use tyvek but a picture would be nice.
The only thing that looks suspicious to me is this:
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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Re: 14 days after g2g MS one shake... should I worry? [Re: enlightenment]
#23965965 - 12/29/16 04:01 PM (7 years, 1 month ago) |
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Quote:
enlightenment said: Strange... Could you post a pic of your lids please? I know you use tyvek but a picture would be nice.
The only thing that looks suspicious to me is this:

I'm sorry I'll be ready to post pics in 3 days but the point you pointed out could be due to the recent shake... (unless my newbie eye is missing something) but anyways I shook all the jars just to redistribute the mycelium for the second time, will report in 3 days!
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mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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Re: 14 days after g2g MS one shake... should I worry? [Re: mushhiehunter]
#23975302 - 01/02/17 07:55 AM (7 years, 28 days ago) |
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I guess the second shake was exactly what they needed, (4 days after second shake)..
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danjaone
Entrepreninja


Registered: 03/19/10
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Re: 14 days after g2g MS one shake... should I worry? [Re: mushhiehunter]
#23976486 - 01/02/17 03:15 PM (7 years, 28 days ago) |
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ive found that just one shake it good if g2g. just a good long shake. make several inoc points in the jar by shaking it for an extended period then 7-14 days later your good to go
-------------------- Even chaos has a melody.... act accordningly
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