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OfflineEdmunter
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LC
    #23960694 - 12/27/16 02:38 PM (7 years, 2 months ago)

I have 2 Liquid cultures and want to inoc some grains soon.  They both look thick and healthy  but I am using micropore on the lids and need to give them a good shake b4 loading up the syringes.

Whats the best plan of action?


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Offlineenlightenment
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Re: LC [Re: Edmunter] * 1
    #23960701 - 12/27/16 02:43 PM (7 years, 2 months ago)

Shake it with your thumb on the filter hole (use a washed and sanitized glove). Suck it in and hope for the best.... Make more than one syringes from this. I would not use this LC jar again if the filter got wet.

It is not good to do this but I don't see much possibilities here.

You could try to tilt the jar and suck it in without shaking.

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Re: LC [Re: enlightenment]
    #23960714 - 12/27/16 02:51 PM (7 years, 2 months ago)

If I sprayed the top with iso before shaking and left in the Sab maybe.    Or suck up 3 or 4 syringes (60cl)  and use the rest by pouring it in some subs.. like a LI

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Offlineenlightenment
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Re: LC [Re: Edmunter]
    #23960725 - 12/27/16 02:56 PM (7 years, 2 months ago)

Usually you use pasty plates if I remember it right. Why didn't you made EzLC? nvm.. You could try to spray it with iso and shake it with your gloved sanitized thumb. Why the thumb? It is possible that the tape goes off if you shake the jar hard. You should test the LC before you use it on multiple jars. LC is nice IMO but it is risky and not easy to do it right.

:goodluck:

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InvisiblebodhisattaMDiscordReddit
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Re: LC [Re: enlightenment] * 1
    #23960753 - 12/27/16 03:04 PM (7 years, 2 months ago)

Fill a syringe with sterilized water then push the plunger down hard so the jet of water cuts up your mycelium

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Offlineenlightenment
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Re: LC [Re: bodhisatta]
    #23960761 - 12/27/16 03:06 PM (7 years, 2 months ago)

Good idea. It should be easier if you suck up some of the uncolonized liquid first. There will be less resistance.

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InvisibleMunchauzen
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Re: LC [Re: enlightenment]
    #23960773 - 12/27/16 03:11 PM (7 years, 2 months ago)

Pour them into empty sterile mason jars and shake them. From there, pour them and say fuck the syringes and ships. Or do the syringe/ship thing, either way.

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Re: LC [Re: Munchauzen]
    #23960809 - 12/27/16 03:24 PM (7 years, 2 months ago)

Quote:

enlightenment said:
Usually you use pasty plates if I remember it right. Why didn't you made EzLC? nvm.. You could try to spray it with iso and shake it with your gloved sanitized thumb. Why the thumb? It is possible that the tape goes off if you shake the jar hard. You should test the LC before you use it on multiple jars. LC is nice IMO but it is risky and not easy to do it right.

:goodluck:





I tried it twice and failed both times.  I must of either contaminated with the injection or made dirty plates.  Both looked clean but I was using DFA and Pasty said that can cause problems if not sterilised long enough.  I will go back to it but im having contamination problems all round and I think its mt old PC giving up.

I only really want the P. Mex but thought id make a PE LC too.

I will do as above and test them first.

Quote:

Munchauzen said:
Pour them into empty sterile mason jars and shake them. From there, pour them and say fuck the syringes and ships. Or do the syringe/ship thing, either way.




Hmm, good idea.  I might do both and use 4 smaller jars.  2 per lager LC.  Then I can use one for pouring and 1 for filling syringes with only a lid with a Ship and no non appropriate filter.

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InvisibleMunchauzen
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Re: LC [Re: Edmunter] * 1
    #23960859 - 12/27/16 03:55 PM (7 years, 2 months ago)

Might need to add a small amount of watwr in the empty jar to get it sterilized. Steam is the great sterilizer at work in a PC

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