|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 19 days
|
LC
#23960694 - 12/27/16 02:38 PM (7 years, 2 months ago) |
|
|
I have 2 Liquid cultures and want to inoc some grains soon. They both look thick and healthy but I am using micropore on the lids and need to give them a good shake b4 loading up the syringes.
Whats the best plan of action?
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
|
|
Shake it with your thumb on the filter hole (use a washed and sanitized glove). Suck it in and hope for the best.... Make more than one syringes from this. I would not use this LC jar again if the filter got wet.
It is not good to do this but I don't see much possibilities here.
You could try to tilt the jar and suck it in without shaking.
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 19 days
|
|
If I sprayed the top with iso before shaking and left in the Sab maybe. Or suck up 3 or 4 syringes (60cl) and use the rest by pouring it in some subs.. like a LI
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
|
|
Usually you use pasty plates if I remember it right. Why didn't you made EzLC? nvm.. You could try to spray it with iso and shake it with your gloved sanitized thumb. Why the thumb? It is possible that the tape goes off if you shake the jar hard. You should test the LC before you use it on multiple jars. LC is nice IMO but it is risky and not easy to do it right.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
|
|
Fill a syringe with sterilized water then push the plunger down hard so the jet of water cuts up your mycelium
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 9 months, 22 days
|
|
Good idea. It should be easier if you suck up some of the uncolonized liquid first. There will be less resistance.
|
Munchauzen


Registered: 06/22/11
Posts: 14,343
|
|
Pour them into empty sterile mason jars and shake them. From there, pour them and say fuck the syringes and ships. Or do the syringe/ship thing, either way.
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 19 days
|
|
Quote:
enlightenment said: Usually you use pasty plates if I remember it right. Why didn't you made EzLC? nvm.. You could try to spray it with iso and shake it with your gloved sanitized thumb. Why the thumb? It is possible that the tape goes off if you shake the jar hard. You should test the LC before you use it on multiple jars. LC is nice IMO but it is risky and not easy to do it right.

I tried it twice and failed both times. I must of either contaminated with the injection or made dirty plates. Both looked clean but I was using DFA and Pasty said that can cause problems if not sterilised long enough. I will go back to it but im having contamination problems all round and I think its mt old PC giving up.
I only really want the P. Mex but thought id make a PE LC too.
I will do as above and test them first.
Quote:
Munchauzen said: Pour them into empty sterile mason jars and shake them. From there, pour them and say fuck the syringes and ships. Or do the syringe/ship thing, either way.
Hmm, good idea. I might do both and use 4 smaller jars. 2 per lager LC. Then I can use one for pouring and 1 for filling syringes with only a lid with a Ship and no non appropriate filter.
|
Munchauzen


Registered: 06/22/11
Posts: 14,343
|
|
Might need to add a small amount of watwr in the empty jar to get it sterilized. Steam is the great sterilizer at work in a PC
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 148 topic views. 31 members, 204 guests and 39 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|