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Offlinestripe208
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First time agar work, need help
    #23958077 - 12/26/16 10:59 AM (7 years, 1 month ago)

I successfully transferred live spawn to 2 brf agar substitute platest, I see lots of different places I think I should cut to start sectoring but to be honesty I'm not too sure what exactly I'm looking for... if someone can help out here's a pic


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InvisibleCultiV8
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Re: First time agar work, need help [Re: stripe208]
    #23958328 - 12/26/16 01:10 PM (7 years, 1 month ago)

Anywhere where you see nice, even radial growth can be transferred to another plate for sectoring. The image below is where I'd cut from. Be sure to label as you go, it's easy to forget which cut came from which sector!



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Offlinestripe208
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Re: First time agar work, need help [Re: CultiV8]
    #23958339 - 12/26/16 01:18 PM (7 years, 1 month ago)

Thank you so much, I've been waiting all morning for a reply before I did the transfer.


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OfflineThekhatinthehat
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Re: First time agar work, need help [Re: stripe208]
    #23959061 - 12/26/16 07:18 PM (7 years, 1 month ago)

Looks good


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Offlinekeifnnugs
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Re: First time agar work, need help [Re: Thekhatinthehat]
    #23959097 - 12/26/16 07:35 PM (7 years, 1 month ago)

I would take a piece from each of the points CultiV8 pointed out and pick the ones with the best growth.. Or after each of them almost fill a plate tiger drop them into qt jars and make those your masters


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Offlinestripe208
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Re: First time agar work, need help [Re: keifnnugs]
    #23962178 - 12/28/16 08:45 AM (7 years, 1 month ago)

Lol I know this is probably the noobiest question I can ask but I really don't know, what are grain masters generally used for exactly?


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Offlineenlightenment
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Re: First time agar work, need help [Re: stripe208]
    #23962191 - 12/28/16 08:53 AM (7 years, 1 month ago)

They are used for grain to grain (G2G) transfers.
The grain master jar should be healthy and fully colonized.
You can use one master jar to inoculate multiple grain jars.


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OfflineTheblingbling
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Re: First time agar work, need help [Re: enlightenment]
    #23962351 - 12/28/16 10:08 AM (7 years, 1 month ago)

looks good


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Offlinestripe208
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Re: First time agar work, need help [Re: Theblingbling]
    #23971267 - 12/31/16 04:21 PM (7 years, 30 days ago)

Time for my next set of cuts, I understand what I'm looking for now but I'd still like to hear from experienced agar workers where they'd cut from...

Also what should I do with the older playes? I don't have time to make spawn right now...


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Offlinestripe208
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Re: First time agar work, need help [Re: stripe208]
    #23972654 - 01/01/17 10:09 AM (7 years, 29 days ago)

Bump, I guess no one saw this for new years lol


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OfflineCrispykoot
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Re: First time agar work, need help [Re: stripe208]
    #23972661 - 01/01/17 10:13 AM (7 years, 29 days ago)

Plates can be stored in an insulated lunchbox with a gel pack in the fridge until you can use them. Long term storage requires the use of slants.


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Offlineenlightenment
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Re: First time agar work, need help [Re: stripe208]
    #23972662 - 01/01/17 10:13 AM (7 years, 29 days ago)

I upload pics in a minute.

Quote:

Also what should I do with the older playes? I don't have time to make spawn right now...




You can use them later when you have time.


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OfflineTheDuder
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Re: First time agar work, need help [Re: enlightenment]
    #23972665 - 01/01/17 10:18 AM (7 years, 29 days ago)

Quote:

CultiV8 said:
Anywhere where you see nice, even radial growth can be transferred to another plate for sectoring. The image below is where I'd cut from. Be sure to label as you go, it's easy to forget which cut came from which sector!







--------------------

|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|


|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|


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Offlineenlightenment
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Re: First time agar work, need help [Re: enlightenment]
    #23972675 - 01/01/17 10:25 AM (7 years, 29 days ago)

Your plates are looking good. The sectors I marked are definitely not the only candidates. I just want to show you what you should look for.







Rhizomorphic mycelium is no guarantee for a good culture. Anyway it is easy to work with rhizomorphic mycelium because sectors are much easier to see.

I like to select fast growing and rhizomorphic sectors.
If my goal is a isolate I cut out very small pieces.


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InvisiblemushboyMDiscord
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Re: First time agar work, need help [Re: enlightenment]
    #23972681 - 01/01/17 10:27 AM (7 years, 29 days ago)

damn my cuts are like twice that size. good lookin out enlightenment:thumbup:


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OfflineKenetic
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Re: First time agar work, need help [Re: CultiV8]
    #23972953 - 01/01/17 12:51 PM (7 years, 29 days ago)

Youve got mold at 6 o'clock


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