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Offlinenoland
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Any mycelium able to transfer? Or too early to tell ??
    #23955083 - 12/24/16 08:39 PM (7 years, 1 month ago)

I had an empty bag of leftover GT cubes that had a bunch of debris/dust in the bottom, and for shits and giggles I decided to transfer some of that to an agar plate to see if any would potentially clone.





These were all dried specimens stored in a bag with a desiccant pack. I did three different distilled water washes and filtered the 'dust' between pours. From what I've read, you need to do a transfer of healthy mycelium early before any contamination takes over. What I'm wondering is if I have any good mycelium to transfer, or is it all contamination? I've done some research to see what I should be looking for, but I don't really have a specific eye in pinpointing this stuff. So I thought I'd ask the community's opinion. Thanks in advance! (Note: These are the same dishes, just different lighting.)


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InvisibleSubnet Mask
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: noland]
    #23955099 - 12/24/16 08:48 PM (7 years, 1 month ago)

How long ago did you make the plates?

Looks like mold spores in the second plate.


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InvisibleMycolorado
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Subnet Mask]
    #23955102 - 12/24/16 08:49 PM (7 years, 1 month ago)

That plate is completely covered in mold.


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InvisiblemushboyMDiscord
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Mycolorado]
    #23955112 - 12/24/16 08:55 PM (7 years, 1 month ago)

Quote:

Mycolorado said:
That plate is completely covered in mold.




yah. thats a fucked up plate.

so you wanted to clone tissue from dried 'mushroom shake'?


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InvisibleMycolorado
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: mushboy]
    #23955119 - 12/24/16 08:59 PM (7 years, 1 month ago)

Just noticed you have that plate open.  Regardless of what you currently have growing there, that's a big no-no, not to mention it looks like it's on the floor on carpet.  Just something to keep in mind for the future.


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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Subnet Mask]
    #23955128 - 12/24/16 09:03 PM (7 years, 1 month ago)

I missed the clone comment.  I figured he was trying to germinate spores.

At least you're using agar.  Get yourself a syringe and hit the ground running.


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Offlinenoland
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Subnet Mask]
    #23955140 - 12/24/16 09:07 PM (7 years, 1 month ago)

Like I said, it was just for fun. I had no hopes for anything that might have grown. I read it was possible to clone dried tissue, and I thought there may have been something left behind. As inane as it sounds, I thought it'd be interesting to see if anything grew. Thanks for the input!


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InvisibleZiran
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: noland]
    #23955205 - 12/24/16 09:54 PM (7 years, 1 month ago)

Quote:

noland said:
I had an empty bag of leftover GT cubes that had a bunch of debris/dust in the bottom, and for shits and giggles I decided to transfer some of that to an agar plate to see if any would potentially clone.





These were all dried specimens stored in a bag with a desiccant pack. I did three different distilled water washes and filtered the 'dust' between pours. From what I've read, you need to do a transfer of healthy mycelium early before any contamination takes over. What I'm wondering is if I have any good mycelium to transfer, or is it all contamination? I've done some research to see what I should be looking for, but I don't really have a specific eye in pinpointing this stuff. So I thought I'd ask the community's opinion. Thanks in advance! (Note: These are the same dishes, just different lighting.)




Those are both molds.


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OfflineLobi
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Ziran]
    #23955238 - 12/24/16 10:06 PM (7 years, 1 month ago)

Doesn't look like their is anything to save in these plates. Transfer as soon as possible and limit the amount of contact to the plate from the inoculant.


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