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noland
Renegade


Registered: 12/08/16
Posts: 66
Last seen: 6 years, 7 months
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Any mycelium able to transfer? Or too early to tell ??
#23955083 - 12/24/16 08:39 PM (7 years, 1 month ago) |
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I had an empty bag of leftover GT cubes that had a bunch of debris/dust in the bottom, and for shits and giggles I decided to transfer some of that to an agar plate to see if any would potentially clone.


These were all dried specimens stored in a bag with a desiccant pack. I did three different distilled water washes and filtered the 'dust' between pours. From what I've read, you need to do a transfer of healthy mycelium early before any contamination takes over. What I'm wondering is if I have any good mycelium to transfer, or is it all contamination? I've done some research to see what I should be looking for, but I don't really have a specific eye in pinpointing this stuff. So I thought I'd ask the community's opinion. Thanks in advance! (Note: These are the same dishes, just different lighting.)
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Subnet Mask


Registered: 05/08/15
Posts: 903
Loc:
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: noland]
#23955099 - 12/24/16 08:48 PM (7 years, 1 month ago) |
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How long ago did you make the plates?
Looks like mold spores in the second plate.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Subnet Mask]
#23955102 - 12/24/16 08:49 PM (7 years, 1 month ago) |
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That plate is completely covered in mold.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Mycolorado]
#23955112 - 12/24/16 08:55 PM (7 years, 1 month ago) |
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Quote:
Mycolorado said: That plate is completely covered in mold.
yah. thats a fucked up plate.
so you wanted to clone tissue from dried 'mushroom shake'?
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: mushboy]
#23955119 - 12/24/16 08:59 PM (7 years, 1 month ago) |
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Just noticed you have that plate open. Regardless of what you currently have growing there, that's a big no-no, not to mention it looks like it's on the floor on carpet. Just something to keep in mind for the future.
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Subnet Mask


Registered: 05/08/15
Posts: 903
Loc:
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Subnet Mask]
#23955128 - 12/24/16 09:03 PM (7 years, 1 month ago) |
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I missed the clone comment. I figured he was trying to germinate spores.
At least you're using agar. Get yourself a syringe and hit the ground running.
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noland
Renegade


Registered: 12/08/16
Posts: 66
Last seen: 6 years, 7 months
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Subnet Mask]
#23955140 - 12/24/16 09:07 PM (7 years, 1 month ago) |
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Like I said, it was just for fun. I had no hopes for anything that might have grown. I read it was possible to clone dried tissue, and I thought there may have been something left behind. As inane as it sounds, I thought it'd be interesting to see if anything grew. Thanks for the input!
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,030
Loc: Temple of Time
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: noland]
#23955205 - 12/24/16 09:54 PM (7 years, 1 month ago) |
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Quote:
noland said: I had an empty bag of leftover GT cubes that had a bunch of debris/dust in the bottom, and for shits and giggles I decided to transfer some of that to an agar plate to see if any would potentially clone.


These were all dried specimens stored in a bag with a desiccant pack. I did three different distilled water washes and filtered the 'dust' between pours. From what I've read, you need to do a transfer of healthy mycelium early before any contamination takes over. What I'm wondering is if I have any good mycelium to transfer, or is it all contamination? I've done some research to see what I should be looking for, but I don't really have a specific eye in pinpointing this stuff. So I thought I'd ask the community's opinion. Thanks in advance! (Note: These are the same dishes, just different lighting.)
Those are both molds.
-------------------- Song Of Healing
Updated Pf Tek Guide Ziran's Teks AMU Q&A Thread The Chinese word for nature is zìrán and it means that of which is of itself.

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Lobi
Bushido



Registered: 02/06/16
Posts: 1,231
Loc: PNW
Last seen: 4 months, 29 days
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Re: Any mycelium able to transfer? Or too early to tell ?? [Re: Ziran]
#23955238 - 12/24/16 10:06 PM (7 years, 1 month ago) |
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Doesn't look like their is anything to save in these plates. Transfer as soon as possible and limit the amount of contact to the plate from the inoculant.
-------------------- The bonds and ties of the life we know break easily. But through eternity one bond remains; the bond of fellowship. The fellowship of atoms, of star dust in its endless flight, of suns and worlds, of gods and men. The clasped hands of comradeship unite in a bond eternal; the fellowship of spirit. - My High Quality Lo-Fi Beats - - MushroomCultivation Compendium - - Doing Bulk w/ No PC - more about my music
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