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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Using empty petri dishes once they have been taken out of sterile bag
#23954160 - 12/24/16 12:41 PM (7 years, 1 month ago) |
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Hey back with another noob question tried search button didn't find what I was looking for.
My question is this I have 5 remaining empty petri dishes from the bag of 20 that were not used for pouring. I intend to make a new batch this time not using honey so I get faster growth, these plates I intend to be used with any luck for my transfer to grains.
From my understanding once the petri dishes have been taken out of the bag they are now no longer sterile( I'll add that I took them out of the bag in open air and then put them into a freezer bag for later use).
Can I even use these empty plates now if I intend to use them for transfers to get a clean culture? Is there a way to get them sterile again?
Can I simply put them back in the glove box and wipe them down with 70% iso inside and out prior to pouring and they will be fine for transfers? Or is there a process I should be following for unused, non poured petri dishes which were out in the open air.
I'll add that I didn't remove the lids on any of them but that doesn't necessarily mean shit.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: Using empty petri dishes once they have been taken out of sterile bag [Re: Dabrit]
#23954182 - 12/24/16 12:56 PM (7 years, 1 month ago) |
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They should be fine as long as you didn't open them. Pour your agar then let them sit for a week @ room temp to see if anything colonized before using them would be my only suggestion.
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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Re: Using empty petri dishes once they have been taken out of sterile bag [Re: liloldme]
#23954228 - 12/24/16 01:19 PM (7 years, 1 month ago) |
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Do not wipe them with iso. I recommend to pour all 20 dishes at once. (If you use packs of 20) You can store them poured for a few weeks (the medium dries slowly). If you want to store them poured you should 'seal' them with parafilm.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Using empty petri dishes once they have been taken out of sterile bag [Re: enlightenment]
#23954262 - 12/24/16 01:34 PM (7 years, 1 month ago) |
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: Using empty petri dishes once they have been taken out of sterile bag [Re: enlightenment]
#23954263 - 12/24/16 01:34 PM (7 years, 1 month ago) |
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Quote:
liloldme said: They should be fine as long as you didn't open them. Pour your agar then let them sit for a week @ room temp to see if anything colonized before using them would be my only suggestion.
Quote:
enlightenment said:

Do not wipe them with iso. I recommend to pour all 20 dishes at once. (If you use packs of 20) You can store them poured for a few weeks (the medium dries slowly). If you want to store them poured you should 'seal' them with parafilm.
Duly noted thanks guys. For this batch pouring all 20 wouldn't have worked out as I had honey in the last batch which definitely seems to slow growth in the plates so I was always going to need to make another batch of different agar using raw sugar.
But going forward I'll be sure to keep enough packs of petri's around.
And you have to use parafilm? Where to buy that stuff? It's expensive is it not?
Can't I just use saram wrap or electrical tape or something else that's around the house ?
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Using empty petri dishes once they have been taken out of sterile bag [Re: Dabrit]
#23954267 - 12/24/16 01:36 PM (7 years, 1 month ago) |
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Find my agar Tek in my signature
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 6 months, 29 days
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Re: Using empty petri dishes once they have been taken out of sterile bag [Re: bodhisatta]
#23954402 - 12/24/16 02:39 PM (7 years, 1 month ago) |
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Quote:
bodhisatta said: Find my agar Tek in my signature
Cheers man
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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