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Offlinetripinballs
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Registered: 09/07/16
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Last seen: 6 years, 9 months
Tissue Viability for biopsy
    #23953329 - 12/24/16 03:41 AM (7 years, 1 month ago)

I've got my fresh mushrooms in the fridge in a paper bag at the moment, one of which I would like to clone. Will the tissue remain viable for biopsy having being stored in the fridge for three or four days?

I've had a nice first flush. Second grow using cakes, one cake kicked out a fat 60 gram monster. One kicked out a long thin 20 gram shroom. I've not weighed the remained from the flush but they're nice, probably another 20-30g.

I actually ate the 20g shroom, the strength seems pretty good - Golden Teacher one of the cakes is virtually blue! The first grow I used Dutch King and wasn't too impressed, had good knotty mycelium but the strength seemed poor - although it could have been my shitty drying out technique.


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Offlinetump
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Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
Re: Tissue Viability for biopsy [Re: tripinballs]
    #23953337 - 12/24/16 03:52 AM (7 years, 1 month ago)

Yes cut it and put it on agar stat. Growth might be weak so take 3 or 4 samples. And have more plates ready for transfers.


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Offlinewtfcrazymofo
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Registered: 07/26/15
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Re: Tissue Viability for biopsy [Re: tump]
    #23953361 - 12/24/16 04:28 AM (7 years, 1 month ago)

It is good to take a clone.  Tear it in half and take a tiny piece less than the size of a grain of rice with a scraping motion from the center of the stem with your post flamed scalpel and drop it on agar in a sab.

The dutch king syringe is probably slightly contaminated giving your ms grow a hard time, but a drop on agar to clean those dirty spores up by transferring clean growth to new plates will probably result in better potency.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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