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Grimsweeper
don't fear the sweeper


Registered: 01/29/18 
Posts: 4,843
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: rickyswamps]
#26684944 - 05/21/20 06:26 AM (3 years, 8 months ago) |
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-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
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Sockadin



Registered: 01/03/10
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: rickyswamps]
#26689050 - 05/22/20 08:14 PM (3 years, 8 months ago) |
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Quote:
rickyswamps said: I confess that during this virus I started using WAY less alcohol for cultivation and am getting the same results 
I have to say that I am using way more Alcohol but most of it seems to be going I'm my stomach and less on the plates. I still have a theory that people with a blood alcohol level close to .8% are going to be less susceptible to the virus.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: rickyswamps]
#26690107 - 05/23/20 10:31 AM (3 years, 8 months ago) |
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Quote:
rickyswamps said: I confess that during this virus I started using WAY less alcohol for cultivation and am getting the same results 
I've not used alcohol for months: even switched to cold tap water to flame my scalpel... basically just keeps the handle cool... alcohol sanitizes, flame sterilizes: which renders sanitation obsolete... I dont hardly wash my hands: just rub them together briskly front and back to the elbows and go to work... honestly; little to no difference
I confess: I will no longer be using the term FAE (frequent air exchange) and will instead be using, and encouraging others to adopt the term PAE (passive air exchange) instead... I feel frequent air exchange resembles the day and age of fanning and misting cakes in SGFC and performing that archaic and unnecessary dance of balancing conflicting forces... I feel as though passive air exchange better portrays the mechanism behind most modern tek
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A.k.a
Stranger



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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
#26690222 - 05/23/20 11:47 AM (3 years, 8 months ago) |
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I always thought it meant fresh air exchange??
I confess- every place I’ve been is out of ONLY quart jars. I just payed 3.25 each at god damn hobby lobby for four of them to hold me over while I keep searching.
Then on the way home I saw what looked like a huge piece of plastic sheeting probably 20 square feet. I immediately thought oh liner!!!
If it wasn’t in the middle of a main intersection probably would’ve grabbed it.
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LAGM2020     
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Sockadin



Registered: 01/03/10
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
#26690236 - 05/23/20 11:56 AM (3 years, 8 months ago) |
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Yeah I always thought it ment fresh air exchange too, I been on this site for a minuet too.
I like PAE, but who really keeps their tubs or SB closed or slightly latched? I'm opening mine up all the time when I go to fruiting in a shoebox I just remove the lid and lightly drape a piece of plastic wrap over the edges leaving cracks here and there.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Sockadin]
#26690355 - 05/23/20 01:04 PM (3 years, 8 months ago) |
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I'll confess too that imo MS should stand for 'multi strain' and not 'multi spore' i mean it's not even possible to grow mushrooms from just one spore of course it's always gonna be multispore when growing with spores but that doesn't necessarily mean that it's multi strain. When inoculating with spore syringes or multi strain cultures then it's a MS grow. Who's with me?
@caps I totally agree with you and I don't mind changing the terms even if it will become a little confusing short term but in the long term it might turn out to be very practical.
I also have opinions on the calculating of how many grams dried from 1 'qt' of spwan. Someone's qt of spawn contains ~500ml grain and someone's qt of spawn contains ~800ml grain and that's a huge difference and is in no way a scientific way of measuring success so I'm all confused about why we haven't come up with a better method to gauge yields. In my case 1qt of spawn is exactly 2 'qt jars' (my jars are 750ml)
-------------------- Cakes inside Water Tub
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Grimsweeper
don't fear the sweeper


Registered: 01/29/18 
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Sockadin]
#26690357 - 05/23/20 01:05 PM (3 years, 8 months ago) |
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First time I've heard FAE called frequent air exchange
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
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A.k.a
Stranger



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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Grimsweeper]
#26690398 - 05/23/20 01:24 PM (3 years, 8 months ago) |
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Ms is just a good term to mean “not a clone”.
Then it gets into the whole debate over whether or not a clone has multiple strains.
Pretty good point on the quart thing. Seems like most people fill them up to near enough the same amount though. I usually shoot for just below where the neck starts to taper.
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LAGM2020     
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Sockadin



Registered: 01/03/10
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: A.k.a]
#26690422 - 05/23/20 01:34 PM (3 years, 8 months ago) |
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Is a clone really a clone though?
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Sockadin]
#26690460 - 05/23/20 01:55 PM (3 years, 8 months ago) |
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Well perhaps I misread somewhere... never the less: even fresh air exchange is misleading... unless you're outdoors lol... and mateah; 100% agree with you, always thought it was more accurate terminology as well...
Sockadin: a clone is a clone, but not necessarily a single strain... single fruit will still contain hundreds if not thousands of individual strains... the size of the tissue sample you take will play a role in how many you end up with, as well as the degree of isolation prior to fruiting... I postulate that a true mono-strain culture doesnt exist for very long: I feel like mutation occurs quite easily and likely an evolutionary trait in order to adapt to different conditional stresses
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Sockadin]
#26690473 - 05/23/20 02:00 PM (3 years, 8 months ago) |
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Quote:
A.k.a said: Pretty good point on the quart thing. Seems like most people fill them up to near enough the same amount though. I usually shoot for just below where the neck starts to taper.
But the point is exactly that it varies widely between one's qt of spawn and another ones we can all agree that a 1000ml jar filled 3/4 full is about 750ml of grain but then again someone who puts 5qt jars in a tub might use 4000ml grain and someone who puts 5qtbin a tub might use 2500ml grain and that's a huge difference and is in no way a scientific way to gauge yields. If everyone used the same amount of grain in their 'qt' jars i wouldn't have an opinion on this in that case why don't we say 15-30g/qt of spawn? That would be equally confusing to determine and compare yields imo.
Quote:
Sockadin said: Is a clone really a clone though?
That's a good question as well. I've often wondered when I go to transfer a clone culture onto multiple plates for expansion let's say 15 plates then how would I know that I have the same strains in every plate? Or course I can only judge with my eyes if the fruits and performance will vary hugely of not notecably at all. In most cases a clone will be a culture with drastically isolated strain containing a handful of them and that's what we'd call a 'clone' and the multi strain term will be reserved for cultures containing a huge variety of strains that are fairly easy to notice due to the varieties in growth. In jokers opinions of course
-------------------- Cakes inside Water Tub
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Caps McGee
Grandaddy Smurfshack



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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Mateja]
#26690481 - 05/23/20 02:04 PM (3 years, 8 months ago) |
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You don't have the same genetics on different plates... similar perhaps, but it's kind of my thing to do sector isolations and to clone clones... I've never seen a culture stay monosectoral for more than a few transfers: almost like it unpacks genetic code over time? I'm 26 and 28 transfers out from OG clone cultures and STILL seeing new sectors form each generation... I've seen multiple instances where theres a diamond in the rp8gh so to speak, when a powerhouse culture comes out, dominates for 3 or 4 generations, and then fizzles back to average performance... anyone else experience this? Otherwise healthy and clean culture
I do 3 full quarts by volume with a pitcher... never occured to weigh it but I see commercial farmers weighing bags
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A.k.a
Stranger



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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
#26690506 - 05/23/20 02:13 PM (3 years, 8 months ago) |
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I kinda worry about ruining an awesome culture by narrowing genetics during transfers too.
I started cutting the middle out and putting it on a new plate but idk how long that’ll work before it gets old.
I was thinking that maybe to expand a plate where you want to keep it as is cutting a ring around the center would work.
That way you’d get a piece of every sector on the plate. Of course they’d get progressively larger so you could probably only do a few before the ring you’d transfer would be too big.
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LAGM2020     
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: A.k.a] 1
#26690534 - 05/23/20 02:24 PM (3 years, 8 months ago) |
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Best way to stay close to a culture is to use it as a master: transfer out once (repeatedly] and then expand the resulting plate... it wont be exactly the same, but it's closer to the tree so to speak
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RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
#26690948 - 05/23/20 06:25 PM (3 years, 8 months ago) |
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Isn't that what master plates and slants are all about?
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Doc9151
Mycologist



Registered: 02/23/17
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: RyeHumor]
#26692480 - 05/24/20 11:38 AM (3 years, 8 months ago) |
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Quote:
RyeHumor said: Isn't that what master plates and slants are all about?
yes and no, slants are for long term storage with low space requirements imo and a master is just that.
--------------------
  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Doc9151] 1
#26693236 - 05/24/20 05:39 PM (3 years, 8 months ago) |
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Yeah ... a slant isn't so much for propagation as for long term storage with very little space requirement... the thickness of the agar coupled with the limited surface area allows it to maintain moisture for a long period of time: which is the biggest issue trying to store long term on a plate... they dry out
Point was, you want to preserve as much of the genetic material from the master as possible, so I expand transfers taken from it, not the master itself... eventually, once the plate is worn out, I take several from the master and pick the nicest to begin using as a new master... that said, I've also just continued to transfer and not kept a master with similar results... I'm really starting to lean in the direction that a true monostrain isolate is nearly impossible due to genetic mutations over time
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Sockadin



Registered: 01/03/10
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
#26693320 - 05/24/20 06:08 PM (3 years, 8 months ago) |
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Alright so based on this concept of you are using D3monics punch Tek. You can go for the largest punch in the set for slants after cleaning up a clone and the smallest for culture propagation. Am I following this correctly? I guess what I am asking is if we want to slant a clone culture do we want to take the largest majority of myclieum from the healthiest looking sector, and when we are cleaning a clone do we want the smallest?
I mean if you clone Joe Rogan you are copying his genome exactly, but with mushrooms. If you clone a fruit, you are copying the fruit and all of the fruits genetic material before it. Like instead of an exact copy you get a copy of its family tree so to speak...?
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TedsDead


Registered: 01/03/17
Posts: 4,998
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Sockadin]
#26693369 - 05/24/20 06:29 PM (3 years, 8 months ago) |
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-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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Sockadin



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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: TedsDead]
#26693448 - 05/24/20 07:01 PM (3 years, 8 months ago) |
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Pretty dope Ted. I missed so much info between 2017-2019. Honestly don't even know if that was done in that time.
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