Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: < First | < Back | 916 | 917 | 918 | 919 | 920 | 921 | 922 | 923 | 924 | 925 | 926 | 927 | 928 | 929 | 930 | 931 | 932 | 933 | 934 | 935 | 936 | Next > | Last >
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: AyePlus]
    #26251710 - 10/14/19 12:11 PM (4 years, 3 months ago)

agreed. I'd have dried them and then did a little one gram (or so) control dose to see how they worked. How long did they sit?


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: verum subsequentis] * 1
    #26251732 - 10/14/19 12:22 PM (4 years, 3 months ago)

I confess, I'm ready to kill my landlord again. We're having a cold snap/wave come through, and my internal temps are dipping into the high 50s at night. It's fucking up everything I'm growing right now... and I can't deal with this again like last year.

So, yeah, I'm house hunting. I'm over landlords.


Extras: Filter Print Post Top
OfflinePsiloPsychIn
PsiloPsychIn
I'm a teapot User Gallery


Registered: 06/17/14
Posts: 8,182
Loc: up north
Last seen: 3 days, 4 hours
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: ShaperDreaming]
    #26251750 - 10/14/19 12:39 PM (4 years, 3 months ago)

Good luck with your house hunting ShaperDreaming. It’s awesome having your own place... except being responsible when anything needs upkeep! πŸ˜‚

I confess, even though it feels wasteful - I like a fresh blade!



Especially when I’m taking clone tissue samples!


--------------------
What are they saying? Listen carefully, it might be something you need to hear...





Extras: Filter Print Post Top
OfflineMH5109
Stranger
 User Gallery

Registered: 09/27/17
Posts: 1,385
Loc: America Flag
Last seen: 2 years, 11 months
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: PsiloPsychIn]
    #26251763 - 10/14/19 12:47 PM (4 years, 3 months ago)

I always use a fresh blade, everytime I get into the sab. When they are clean and sharp stuff doesn't stick to the blade like it does after it gets tarnished and carbon buildup on it. Blades are cheap and recyclable.


--------------------




Extras: Filter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,224
Last seen: 11 hours, 14 minutes
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: MH5109]
    #26255452 - 10/16/19 07:17 AM (4 years, 3 months ago)

I confess
ordered my 1st ape syringe this morning, lookin forward to the PE adventures


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Filter Print Post Top
Invisibleforagedfungus
 User Gallery


Registered: 09/30/13
Posts: 1,849
Loc: out there
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Camera93]
    #26255474 - 10/16/19 07:38 AM (4 years, 3 months ago)

Quote:

foragedfungus said:
Flow hood, big pc, and most of my myco stuff are all sitting in a storage unit for a few months.

Today I set up a space in the basement, and used my old SAB for the first time in years...
Confession: Felt like a shaky handed noob. Couldn't see what I was doing. Hit the top of the box a couple of times. Probably fucked up half my plates.
:noob:




After a week, 7 out of 11 dishes were covered in satellites and bacterial splooge.

I did another round of transfers a few days ago. Paid much more attention to technique and moving slow and smooth.
It's been 3 days and all 10 out of 10 plates look perfect. :cool:


Extras: Filter Print Post Top
OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 9 hours, 23 minutes
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: foragedfungus]
    #26255489 - 10/16/19 07:47 AM (4 years, 3 months ago)

I confess I think I'm gonna get a bottle of antibiotic agar powder to aid in cleaning up cultures, after years of being anti-antibiotic use for agar... Seems like no matter what I do I have bacteria riding along with 2 of my cultures


Extras: Filter Print Post Top
InvisibleAyePlusS
Stony Danza
Male User Gallery


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Leftfield420]
    #26255649 - 10/16/19 09:13 AM (4 years, 3 months ago)

Did you try hot pour or sandwich techniques?

Get an nice thick layer of agar between you and the culture, the myc should be able to more through it without the bacteria.


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: AyePlus]
    #26255747 - 10/16/19 10:08 AM (4 years, 3 months ago)

I confess! I decided to slow down my growing until I finish my house purchase. I'm tired of not having control over my conditions inside my building, and now I'm already dipping into the high 50s at night here and it's fucking up my cultures :hissyfit: I'm going to still grow, but damn! I'm just super annoyed at all this, and I pay way too much money for my apartment to be this fucking complicated.

Quote:

Camera93 said:
I confess
ordered my 1st ape syringe this morning, lookin forward to the PE adventures



APE > PE

But, I found APE to be a huge pain in the ass to get looking good on agar, so be prepared for funky myc and many transfers :heart:

Quote:

AyePlus said:
Did you try hot pour or sandwich techniques?



Or even T-Gel?


Extras: Filter Print Post Top
InvisibleAyePlusS
Stony Danza
Male User Gallery


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: ShaperDreaming] * 1
    #26255774 - 10/16/19 10:21 AM (4 years, 3 months ago)

Same Idea but kinda in the middle of a hot pour/ sandwich; Transferring a wedge to a dish that hasnt fully hardened yet and bury it in the solidifying agar, discovered this one on accident but now I use it regularly for bacterial cleanup.

β€œPanini tek” lol


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: AyePlus]
    #26255784 - 10/16/19 10:24 AM (4 years, 3 months ago)

Oh! I try to do that for my clones because I've always found I get the best results when my clone starts "in" the agar. Good to know I should for transfers too :laugh: Thanks.


Extras: Filter Print Post Top
InvisibleAyePlusS
Stony Danza
Male User Gallery


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: ShaperDreaming]
    #26255885 - 10/16/19 11:21 AM (4 years, 3 months ago)

Only if you’re concerned about bacteria or other hidden contams, I try to place the transfer upside down and push it down to the bottom so the myc is as deep as possible


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 9 hours, 23 minutes
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: AyePlus]
    #26255920 - 10/16/19 11:43 AM (4 years, 3 months ago)

I have tried the agar sandwhich... May try putting a wedge in a empty dish and pour agar over that wedge..


Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Leftfield420]
    #26255923 - 10/16/19 11:44 AM (4 years, 3 months ago)

take spore print on petri, pour agar over top

profit


Extras: Filter Print Post Top
InvisibleTeaforTwo
Flight Attendant
I'm a teapot


Folding@home Statistics
Registered: 09/22/19
Posts: 1,005
Loc: Psychonaut City, Mile Hi!
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Psilosopherr]
    #26255994 - 10/16/19 12:21 PM (4 years, 3 months ago)

Quote:

Psilosopherr said:
take spore print on petri, pour agar over top

profit



:jah: friggin awesome idea.. ima do this!

:sunsalutation:


--------------------
See a mush cult pic you love? add it here: January 2020 PoM nominations thread! (up vote your faves to ensure they are included in final vote!). Community selected winner receives a 6mo Supporter account upgrade and/or other prizes from our awesome admin team!

Looking for something? Try Psilosopherr's Tek Compendium and pixelpopper's essentialzzz ForTheWin!

Be well, friend. Stay Strong and Beautiful, Always, and in All Ways~
Look Forward, Seek Truth, Find Real, Go Further~


Extras: Filter Print Post Top
InvisibleAyePlusS
Stony Danza
Male User Gallery


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Leftfield420]
    #26256111 - 10/16/19 01:02 PM (4 years, 3 months ago)

Quote:

Leftfield420 said:
I have tried the agar sandwhich... May try putting a wedge in a empty dish and pour agar over that wedge..




That would work but I’d just Just pour agar over the dish the culture is already in, no need to use another one.


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 9 hours, 23 minutes
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: AyePlus]
    #26256140 - 10/16/19 01:14 PM (4 years, 3 months ago)

Yeah but if you cut into the bottom layer of agar taking the next set of transfers you'd be potentially carrying that contam to the next dish unintentionally

I have put transfers in blank dishes then poured over them before...worked well enough to get fruits...but still want that antibiotic agar mix from fp just to have as a tool to use


Edited by Leftfield420 (10/16/19 01:17 PM)


Extras: Filter Print Post Top
InvisibleAyePlusS
Stony Danza
Male User Gallery


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Leftfield420]
    #26256239 - 10/16/19 01:55 PM (4 years, 3 months ago)

Thats why its important to pour it deep as possible, and don't do it on a fully colonized plate that way you can still go to the leading edge. Thats one of the advantages of transferring into a still liquid dish, acts like a regular cleaning transfer but with the benefit of trapping the bacteria in the agar.

Basically same idea as what you are doing just slightly different approach and as long as it works it works.

Using a brf puck like agar can be helpful for cleaning up bacteria too, it has a harder time spreading than the myc then you can transfer back to agar.

If you insist on going the antibiotic route to save money instead of buying from FP go to your local pet store and buy the gentamicin they sell for fishes, you add it into the almost cooled agar and swirl before pouring, cant be cooked but I’m pretty sure thats what alan rockefeller uses for cloning from wild tissue samples.


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TeaforTwo]
    #26256271 - 10/16/19 02:06 PM (4 years, 3 months ago)

Quote:

TeaforTwo said:
Quote:

Psilosopherr said:
take spore print on petri, pour agar over top

profit



:jah: friggin awesome idea.. ima do this!

:sunsalutation:



let us know how it goes, I've never done it.


Extras: Filter Print Post Top
OfflineLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,023
Last seen: 9 hours, 23 minutes
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: AyePlus]
    #26256382 - 10/16/19 03:04 PM (4 years, 3 months ago)

Quote:

AyePlus said:
Thats why its important to pour it deep as possible, and don't do it on a fully colonized plate that way you can still go to the leading edge. Thats one of the advantages of transferring into a still liquid dish, acts like a regular cleaning transfer but with the benefit of trapping the bacteria in the agar.

Basically same idea as what you are doing just slightly different approach and as long as it works it works.

Using a brf puck like agar can be helpful for cleaning up bacteria too, it has a harder time spreading than the myc then you can transfer back to agar.

If you insist on going the antibiotic route to save money instead of buying from FP go to your local pet store and buy the gentamicin they sell for fishes, you add it into the almost cooled agar and swirl before pouring, cant be cooked but I’m pretty sure thats what alan rockefeller uses for cloning from wild tissue samples.




I believe he uses a mix of antibiotcs.. Gent beibg one of the mix


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 916 | 917 | 918 | 919 | 920 | 921 | 922 | 923 | 924 | 925 | 926 | 927 | 928 | 929 | 930 | 931 | 932 | 933 | 934 | 935 | 936 | Next > | Last >

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* Semi/automated small bulk substrate grow chamber SixTango 2,492 1 02/09/03 08:58 AM
by Dymentia
* Help! Yellow-Orange Semi-Hard Coating on Cakes Hannibal88 1,823 11 07/25/03 02:41 AM
by TxTec
* random innoculation gray1 892 3 05/03/01 04:10 PM
by gray1
* It's true what they say megaman3 1,735 19 04/22/03 08:37 AM
by cheesenoonions
* Where are the PROFESSIONAL MYCOLOGISTS? psilocybong 2,114 6 03/26/04 11:13 AM
by Anonymous
* Does this mean I am now a mycologist?
( 1 2 all )
Killjoy 4,092 22 07/04/17 07:00 PM
by LotKid
* casing semi-colonized cakes... sBUD 2,572 13 06/29/02 12:45 PM
by TheDnB_One
* Question for intermediate mycologists. Northernsoul 1,953 10 02/18/02 02:56 AM
by moxjet20

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
544,016 topic views. 13 members, 143 guests and 40 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.036 seconds spending 0.015 seconds on 15 queries.