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OfflineSpindlymass
wafer thin.
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Registered: 09/25/17
Posts: 957
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Last seen: 3 months, 25 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: J. Jack Flash]
    #25990545 - 05/13/19 06:21 PM (4 years, 8 months ago)

Jack,

In those moments remember, your life has meaning. Maybe not to you at the time, but to us it does. WE would miss you. WE would care. Who would tell us you were gone?

I confess, I never give myself permission to "let go", to "die" when I'm tripping. There is the slight chance something is really the matter and I have stuff I want to do still.


When someone mentions suicide, people get riled up (and for good reason). I suppose though, there is a difference in thinking about suicide in a passing way and in a serious, practical way.


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enough knowledge to get into trouble. Not nearly enough to get out again.


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Offlinebanishedfromtime
void cult adept

Registered: 06/12/18
Posts: 46
Last seen: 16 days, 6 hours
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Grimsweeper]
    #25990614 - 05/13/19 06:49 PM (4 years, 8 months ago)

Quote:

Grimsweeper said:
I see a lot of germination problem posts and I have to say I've never NOT had germination on standard MEA. Not even once have I streaked a plate with a swab and not had germination with dozens of varities/strains.




just bought some malt extract, so we'll see. what ratios are you using?

what would you recommend doing for inoculation? I have some MS syringes and sterile tips. I find that if I try to use the syringe I end up dishing out way too much and it ends up pooling on the agar, so last time squirted some from the syringe into a shot glass wiped with iso alcohol and dipped the tips in there then inoculated. might be too complicated?


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InvisibleYesum
Furry as Fuc
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Registered: 11/05/12
Posts: 13,124
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: banishedfromtime]
    #25990722 - 05/13/19 07:39 PM (4 years, 8 months ago)

Yeah. Just drop some on the plate. And give the plate a little swirl after. You'll have a bunch of inoculation points.


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InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh 😛
I'm a teapot User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 6,067
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25990864 - 05/13/19 08:30 PM (4 years, 8 months ago)

I confess that I get excited to see fellow Shroomery fam getting the TC Tag honor...This last go around was pretty awesome! Hope to see more soon! Mush love to all! :heart:


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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Spindlymass]
    #25991778 - 05/14/19 08:16 AM (4 years, 8 months ago)

Quote:

Spindlymass said:
When someone mentions suicide, people get riled up (and for good reason). I suppose though, there is a difference in thinking about suicide in a passing way and in a serious, practical way.




thanks everyone who reached out. your concern means a lot, and your relating to it helps for some reason. but i'm feeling silly over it, and feel an explanation is in order. tl;dr i'm frustrated, eager for the next life, but have work yet to do in this one.

mush love, my people.

as for confessing my myco sins. i'm using polypropylene petri dishes, so i don't have to throw them away. so much work to make and wash and reuse them, i'm considering saying fuck it, and just using the disposable ones.


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the j stands for jesus.
2020 new years grow along


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InvisibleYesum
Furry as Fuc
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Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: J. Jack Flash]
    #25991801 - 05/14/19 08:30 AM (4 years, 8 months ago)

Thsts probably the worst part about them is having to sterilize them again to be prepped to be poured. Still. You have me curious now. I didnt realize they made reusable plastic ones.

When you bought them how did you receive them. Box of 500, sleeve of 20, etc. And what was the price on them.

Thsnk you in advance


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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25991823 - 05/14/19 08:51 AM (4 years, 8 months ago)

ebay sold by the dozen, individually wrapped. price went up a fair bit recently. i use the 75mm. really, i accepted several disadvantages in exchange for the reuse feature. i just don't like throwing shit away if i can help it. but laziness is a powerful motivator... as ironic as that is.

i run them no pour style. more or less my current process. still refining it.


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the j stands for jesus.
2020 new years grow along


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InvisibleYesum
Furry as Fuc
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Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: J. Jack Flash]
    #25991849 - 05/14/19 09:11 AM (4 years, 8 months ago)

Have you tried sterilizing them blank and pouring them. I feel like a flowhood would be a huge advantage here obviously. 

I think I might grab some just to have around.  Occasionally I run out of plates in between buying boxes. No pours would suffice but I kinda wanna try those


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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25991871 - 05/14/19 09:20 AM (4 years, 8 months ago)

have not tried. i know glass ones are to be oven sterilized, which obviously ain't wise with these. but now that you mention it, i guess they could be stacked empty, pc'd, then poured in the sab. i guess it's worth a shot. there is much to be desired in the consistency with the no pour approach.

love working with them, tho, for transfers and inoculations and such.


--------------------
 
the j stands for jesus.
2020 new years grow along


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OfflineSpindlymass
wafer thin.
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Registered: 09/25/17
Posts: 957
Loc: Flag
Last seen: 3 months, 25 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25991895 - 05/14/19 09:34 AM (4 years, 8 months ago)

Quote:

Yesum said:
Have you tried sterilizing them blank and pouring them. I feel like a flowhood would be a huge advantage here obviously. 

I think I might grab some just to have around.  Occasionally I run out of plates in between buying boxes. No pours would suffice but I kinda wanna try those






I got a bunch at a surplus store. I put them in a 'Muda bottle', upside-down, and pc them. Flip the bottle in the SAB and pour away. I only stack them 5 or 6 high, because I crack them slightly (which places the stack off kilter) so I can pour them like normal dishes and not have to open each one individually (takes 2 hands) while pouring.

No parafilm etc. needed, they have their own seal. Never had a problem with cultures stalling either.
Nice for mailing too. +'s and -'s of course, like anything.

* when you close them, push all around the perimiter. If you press in the centre, it creates a vacuum.


--------------------
enough knowledge to get into trouble. Not nearly enough to get out again.


Edited by Spindlymass (05/14/19 09:36 AM)


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OfflineGrimsweeper
don't fear the sweeper
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Registered: 01/29/18 Happy 6th Shroomiversary!
Posts: 4,843
Loc: broom closet
Last seen: 7 days, 13 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Spindlymass]
    #25991913 - 05/14/19 09:46 AM (4 years, 8 months ago)

I really dislike wrapping dishes over and over and cutting new 1" segments. What can I search to find the ones that seal?


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When you clean a vacuum cleaner you are a vacuum cleaner
Build yourself a Flow Hood in these 99 simple steps


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OfflineShaperDreaming
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Registered: 10/30/18
Posts: 3,429
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Last seen: 2 years, 3 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Grimsweeper]
    #25991933 - 05/14/19 09:57 AM (4 years, 8 months ago)

Quote:

Grimsweeper said:
I really dislike wrapping dishes over and over and cutting new 1" segments. What can I search to find the ones that seal?



:whathesaid:
:takingnotes:


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OfflineSpindlymass
wafer thin.
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Registered: 09/25/17
Posts: 957
Loc: Flag
Last seen: 3 months, 25 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #25991948 - 05/14/19 10:08 AM (4 years, 8 months ago)

Quote:

ShaperDreaming said:
Quote:

Grimsweeper said:
I really dislike wrapping dishes over and over and cutting new 1" segments. What can I search to find the ones that seal?



:whathesaid:
:takingnotes:





Here is a link to a thread I contributed too. My post has a link to the ones I have. The whole thread has more info.
:cheers:


--------------------
enough knowledge to get into trouble. Not nearly enough to get out again.


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InvisibleYesum
Furry as Fuc
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Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Spindlymass]
    #25991949 - 05/14/19 10:09 AM (4 years, 8 months ago)

Quote:

Spindlymass said:
Quote:

Yesum said:
Have you tried sterilizing them blank and pouring them. I feel like a flowhood would be a huge advantage here obviously. 

I think I might grab some just to have around.  Occasionally I run out of plates in between buying boxes. No pours would suffice but I kinda wanna try those






I got a bunch at a surplus store. I put them in a 'Muda bottle', upside-down, and pc them. Flip the bottle in the SAB and pour away. I only stack them 5 or 6 high, because I crack them slightly (which places the stack off kilter) so I can pour them like normal dishes and not have to open each one individually (takes 2 hands) while pouring.

No parafilm etc. needed, they have their own seal. Never had a problem with cultures stalling either.
Nice for mailing too. +'s and -'s of course, like anything.

* when you close them, push all around the perimiter. If you press in the centre, it creates a vacuum.




Where did these cone from. Those are different then Jack's right?!


Oh cool. Thanks.


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OfflineShaperDreaming
Weirdo
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Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25991960 - 05/14/19 10:15 AM (4 years, 8 months ago)

:woooaaahhh:
This is the reaction my wallet had.


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OfflineSpindlymass
wafer thin.
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Registered: 09/25/17
Posts: 957
Loc: Flag
Last seen: 3 months, 25 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #25991977 - 05/14/19 10:25 AM (4 years, 8 months ago)

Quote:

ShaperDreaming said:
:woooaaahhh:
This is the reaction my wallet had.




:ducklol:
Yep, got lucky at the surplus store! I couldn't believe I found pp5 petris there. I may have jumped around a bit... :smile:


--------------------
enough knowledge to get into trouble. Not nearly enough to get out again.


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OfflineGrimsweeper
don't fear the sweeper
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Registered: 01/29/18 Happy 6th Shroomiversary!
Posts: 4,843
Loc: broom closet
Last seen: 7 days, 13 hours
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #25991986 - 05/14/19 10:31 AM (4 years, 8 months ago)

Quote:

ShaperDreaming said:
:woooaaahhh:
This is the reaction my wallet had.



:whatshesaid: but yeah those are a game changer


--------------------
When you clean a vacuum cleaner you are a vacuum cleaner
Build yourself a Flow Hood in these 99 simple steps


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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Grimsweeper]
    #25992032 - 05/14/19 10:59 AM (4 years, 8 months ago)

those are pretty rad. people on the boards ever do group buys? nine people, 100 plates each for under fifty bucks plus some leg work. or is this in violation of the sponsors agreement? if so, sponsors, we'd like a distributor for these.

i confess, i feel silly for derailing this thread again. it's time to get to work.


--------------------
 
the j stands for jesus.
2020 new years grow along


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InvisibleYesum
Furry as Fuc
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Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: J. Jack Flash]
    #25992073 - 05/14/19 11:22 AM (4 years, 8 months ago)

Right on. I found it.

Dsmn fam those are expensive


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OfflineSpindlymass
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Registered: 09/25/17
Posts: 957
Loc: Flag
Last seen: 3 months, 25 days
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Yesum]
    #25992124 - 05/14/19 11:59 AM (4 years, 8 months ago)

Saw this further down their website ;


Lacons containers in smaller quantities.
esdplasticcontainers.com now has a huge selection of lacons containers with no minimums.


--------------------
enough knowledge to get into trouble. Not nearly enough to get out again.


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