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mullugh
Stranger

Registered: 03/26/18
Posts: 714
Last seen: 1 year, 7 months
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Munchauzen]
#25230536 - 05/26/18 07:40 PM (5 years, 8 months ago) |
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I imagine you'll not stop at psilocybin...Surely there will be more words
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Spindlymass
wafer thin.



Registered: 09/25/17
Posts: 957
Loc:
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: mullugh]
#25233012 - 05/28/18 12:00 AM (5 years, 8 months ago) |
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I confess, I don't know how the hell this happened.

0ne out of twelve mini master slants. All treated the same, PC'd 45 minutes in the same jar that remained unopened until I went to use them...
* Hey theoldclarysage, need a master for your cheese culture?
-------------------- enough knowledge to get into trouble. Not nearly enough to get out again.
Edited by Spindlymass (05/28/18 12:04 AM)
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
Posts: 8,182
Loc: up north
Last seen: 3 days, 4 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Spindlymass]
#25233046 - 05/28/18 12:54 AM (5 years, 8 months ago) |
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I have recently had some contamination problems and I realized it was dirty air was being sucked into my petris via small cracks in my parafilm. The small cracks are not enough. You also need temperature fluxuations.... heating up will displace gas out of the container through small gaps. Temperature drops will cause the gas inside to decrease in volume... sucking air in from outside of the sterile container. If you are unlucky that will include contaminant spores / germs.
I figured this out via the location of contaminants along the periphery of perfectly clean / sterile plates. On close examination I could see small gaps in the parafilm. I also know I get temperature swings... the rest is some rudimentary knowledge of physics and flow dynamics.
I have assumed here that the vial you are showing had some minute means of gas exchange and that it was stored in some place less than sterile.
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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Spindlymass
wafer thin.



Registered: 09/25/17
Posts: 957
Loc:
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: PsiloPsychIn]
#25233073 - 05/28/18 01:17 AM (5 years, 8 months ago) |
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Ah, good call. 
I posted a settle plate pic not too long ago. Lots of shit floating around my room.
I've been thinking. I PC'd them with lids loose for GE in a mason jar, removed from jar (forgot that step in previous post) when cool enough to handle, tightened caps and left at an angle to solidify in a SAB.
Then, once cooled and solidified, back into the jar and lid replaced on jar.
I guess during the cooling period something could have been sucked past the screw cap's seal...
I think next time, I could try to tilt the whole sealed jar of slants. Then, when completely cooled tighten the caps
-------------------- enough knowledge to get into trouble. Not nearly enough to get out again.
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Spotter
Making you all look good

Registered: 05/25/17
Posts: 712
Loc: True North
Last seen: 2 months, 3 days
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Spindlymass]
#25247974 - 06/04/18 12:03 PM (5 years, 7 months ago) |
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I confess: Cordyceps are taking over my myco area. I'm moving edibles outdoors, and I tossed all my ready to use cube spawn in a big diversity tub. Should be a quick, interesting experiment. Too many houseguests this summer to be active with the actives.
-------------------- Its all about the Hericium erinaceus yo. Spotters Resume
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NobodyYouKnow
Someone else



Registered: 08/26/14
Posts: 16,583
Loc: To your south
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Spotter]
#25249181 - 06/04/18 10:45 PM (5 years, 7 months ago) |
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So you'll be inactive with the actives and active with the inactives?
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,070
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: NobodyYouKnow] 1
#25249243 - 06/05/18 12:07 AM (5 years, 7 months ago) |
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Quote:
NobodyYouKnow said: So you'll be inactive with the actives and active with the inactives?

How long have you been waiting for the perfect time to drop that?
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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NobodyYouKnow
Someone else



Registered: 08/26/14
Posts: 16,583
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: elasticaltiger] 1
#25249253 - 06/05/18 12:28 AM (5 years, 7 months ago) |
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Tiger drop.
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
Posts: 8,182
Loc: up north
Last seen: 3 days, 4 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: NobodyYouKnow]
#25249610 - 06/05/18 07:41 AM (5 years, 7 months ago) |
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Niceone NobodyYouKnow!
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: PsiloPsychIn]
#25249776 - 06/05/18 09:43 AM (5 years, 7 months ago) |
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my green house arrived today
not sure if thats a confession or not..things are coming up milhouse!
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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: mushboy]
#25249926 - 06/05/18 11:31 AM (5 years, 7 months ago) |
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Nice Mushboy 
I confess I was a bit apprehensive about agar, but after doing it it’s easy as pie. Used my first ever plates yesterday for three clone tissues and three print swipes. Got a cloning kit on the way, and a inoculation loop. Going to get cereal about this stuff
--------------------
TEKs I Like
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: MadHatter333]
#25250506 - 06/05/18 05:24 PM (5 years, 7 months ago) |
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I confess I’m pretty bummed about these KSSS shoeboxes. Maybe I didn’t use enough spawn/sub or didn’t prep with enough water but all 6 shoeboxes are just putting out nice pinsets of really tiny fruits. Even uncased APE mini blobs in shoeboxes weren’t this small for me..
I figure they all may put out some decent second flushers but I almost don’t even want to bother harvesting any of these, not to mention deal with drying them
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Germs]
#25250612 - 06/05/18 06:08 PM (5 years, 7 months ago) |
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Ksss tend to be really short, but that's abort central. Try to bottom water. That's a killer flush to just lose like that
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 days, 2 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: mushboy]
#25250613 - 06/05/18 06:08 PM (5 years, 7 months ago) |
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Quote:
mushboy said: my green house arrived today
not sure if thats a confession or not..things are coming up milhouse!
Dude, I've been setting mine up this week. I think your gonna love it.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 days, 2 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Asura]
#25250620 - 06/05/18 06:11 PM (5 years, 7 months ago) |
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Hey Germs, same think happened to me on my KSSS grow. It was my first time trying shoeboxes, so it was a bit of a disappointment.
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
Posts: 8,182
Loc: up north
Last seen: 3 days, 4 hours
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Asura]
#25251662 - 06/06/18 09:03 AM (5 years, 7 months ago) |
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Quote:
Asura said: Hey Germs, same think happened to me on my KSSS grow. It was my first time trying shoeboxes, so it was a bit of a disappointment.

Those look like the stubby variety. I don't think it is a problem really. Word is they are very potent!
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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NarkedAt90ft
Deep in the Blue Abyss


Registered: 12/04/13
Posts: 149
Loc: Marianas Trench
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: PsiloPsychIn]
#25252084 - 06/06/18 01:02 PM (5 years, 7 months ago) |
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Made agar last night ..late.. and waited for them to solidify... went back for transfers and to wrap up the extras and they were all still liquid... turns out I doubled up on the malt and didn't put any agar in my MEagar 
Tonight I'll try again.
Nice on the green houses mentioned above - would love to see how you guys end up setting up!
-------------------- ni·tro·gen nar·co·sis noun - a drowsy state induced by breathing air under higher than atmospheric pressure, for example, in deep-sea diving. Also known as Rapture of The Deep. Let's Trade!
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NobodyYouKnow
Someone else



Registered: 08/26/14
Posts: 16,583
Loc: To your south
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: NarkedAt90ft] 1
#25252603 - 06/06/18 05:27 PM (5 years, 7 months ago) |
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I confess, I'm a better hunter than a grower... and I love eating. I don't know if you could grow these anyway. 
  
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mullugh
Stranger

Registered: 03/26/18
Posts: 714
Last seen: 1 year, 7 months
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: NobodyYouKnow]
#25253648 - 06/07/18 07:59 AM (5 years, 7 months ago) |
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Tasty. What are they?
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NobodyYouKnow
Someone else



Registered: 08/26/14
Posts: 16,583
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: mullugh]
#25253655 - 06/07/18 08:07 AM (5 years, 7 months ago) |
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Lactarius deliciosus or saffies as we call them.
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